Abstract: The disclosure is in part directed to crystalline forms of (R)-1?-(3-(3,4-dihydro-1,5-naphthyridin-1(2H)-yl)-1H-pyrazolo[3,4-b]pyrazin-6-yl)-3H-spiro[benzofuran-2,4?-piperidin]-3-amine, its salt, and variants thereof.

Abstract: The present disclosure provides a chassis assembly and a vehicle. The chassis component includes: a chassis provided with a plurality of mounting positions and configured to install a battery module; a quick-release device through which the battery module is installed on the mounting positions, wherein the quick-release device is controlled by a control system; and a voltage electrical connecting assembly electrically connected to the battery module, wherein the voltage electrical connecting assembly is controlled by the control system. The vehicle includes the chassis assembly.

Abstract: A method, system, and motor vehicle for controlling a direct-current fast-charging process of a battery system are provided that significantly improves performance of the direct-current fast-charging process and minimizes condensation issues by utilizing lower coolant temperature for a short duration when a rechargeable energy storage system cell temperature is greater than a specific temperature threshold. The rechargeable energy storage system cell temperature, a rechargeable energy storage system state of charge a rechargeable energy storage system voltage, and a rechargeable energy storage system direct-current fast-charging current are utilized to determine when to utilize the lower coolant temperature when the rechargeable energy storage system cell temperature is greater than a specific temperature threshold instead of using a lower coolant temperature from the beginning of the direct-current fast-charging process.

Abstract: The disclosure is in part directed to crystalline forms of (R)-1?-(3-(3,4-dihydro-1,5-naphthyridin-1(2H)-yl)-1H-pyrazolo[3,4-b]pyrazin-6-yl)-3H-spiro[benzofuran-2,4?-piperidin]-3-amine, its salt, and variants thereof.

Abstract: A method and a system for optoelectronic matching are disclosed. The method comprises the steps of: S1, enabling an electrical port of a first optoelectronic device to auto-negotiate with a first electrical port to obtain the highest supported speed of the first electrical port, and enabling an electrical port of a second optoelectronic device to auto-negotiate with a second electrical port to obtain the highest supported speed of the second electrical port; S2, encapsulating, by an optical port of an optoelectronic device, a current speed and the negotiated highest supported speed of an opposite end in a transmission protocol, and sending the same to an optical port of another optoelectronic device; S3, obtaining a target speed based on the highest supported speed of the first electrical port and the highest supported speed of the second electrical port; S4, determining whether the current speed is equal to the target speed, respectively.

Abstract: A gang drilling machine for drilling a circuit card includes a pair of n and p master drills that are configured to be aligned in registry with respective n and p test vias of the card; pluralities of n and p minion drills that are configured to be aligned in registry with pluralities of n and p live vias of the card; and a controller that is electrically connected to control the n and p master drills and minion drills, and to send and receive electrical signals to and from the card. The controller is configured to: send a query signal to the card; monitor a response signal from the card; determine drilling depth of at least one of the master drills, in response to comparing the response signal to the query signal; and adjust operation of the machine, in response to the determined drilling depth.

Abstract: The present application relates to one or more fusion proteins, comprising a structural domain of SARS-CoV-2 S(Spike) protein. The present application also relates to an immunogenic composition comprising the fusion protein and an application thereof.

Abstract: A battery cell includes an anode, a cathode, a liquid electrolyte, and a battery cell case. The battery cell case is configured to house the anode, the cathode, and the liquid electrolyte and includes a case interior wall arranged proximate one of the anode and the cathode, a battery case ceiling, and a battery case floor catching and collecting the liquid electrolyte due to force of gravity. The case interior wall defines a pattern of surface tension varying between hydrophobic and hydrophilic along the case interior wall between the battery case floor and the battery cell ceiling. The pattern of surface tension thereby facilitates self-propulsion of the liquid electrolyte in opposition to the force of gravity and a predetermined distribution of the liquid electrolyte along the battery cell wall.

Abstract: The invention relates to a method for preparing a solution which has a savory flavor, the method comprising the following steps: (a) providing a mycelium of a basidiomycete, (b) cultivating the mycelium in an aqueous culture liquid for a cultivation duration of 5 to 108 hours, wherein the culture liquid has at least one part of a plant of the genus Allium L.

Abstract: Provided is a PCR primer pair, comprising: a first primer and a second primer, wherein the first primer comprises a first specific sequence and a first random sequence; the first specific sequence is located on 3? end of the first primer, and the first random sequence is located on 5? end of the first primer; the second primer comprises a second specific sequence and a second random sequence, the second specific sequence is located on 3? end of the second primer, and the second random sequence is located on 5? end of the second primer; moreover, the first specific sequence and the second specific sequence are an upstream primer and a downstream primer directed to a target sequence respectively, and the first random sequence and the second random sequence are inverse complementary.

Abstract: Provided are a PCR primer pair and an application thereof.

Abstract: A method and a system for optoelectronic matching are disclosed. The method comprises the steps of: S1, enabling an electrical port of a first optoelectronic device to auto-negotiate with a first electrical port to obtain the highest supported speed of the first electrical port, and enabling an electrical port of a second optoelectronic device to auto-negotiate with a second electrical port to obtain the highest supported speed of the second electrical port; S2, encapsulating, by an optical port of an optoelectronic device, a current speed and the negotiated highest supported speed of an opposite end in a transmission protocol, and sending the same to an optical port of another optoelectronic device; S3, obtaining a target speed based on the highest supported speed of the first electrical port and the highest supported speed of the second electrical port; S4, determining whether the current speed is equal to the target speed, respectively.

Abstract: Embodiments are directed to a method of manufacturing the printed circuit board. The PCB is a multi-layer component, including a dielectric material and an intermediate or second layer adjacently positioned with respect to the dielectric material. The intermediate layer or second layer includes a conductor and fiberglass strands, with the fiberglass strands having an associated orientation. When assembled, the fiberglass and the conductor have a matching orientation and separation distance from a source to a destination.

Abstract: Disclosed are an intermediate drug having synergistic anticancer activity and a polyethylene glycol-coupled synergistic anticancer drug, and a method preparing therefor and use thereof. The intermediate drug has the general structural formula of (I), and the polyethylene glycol-coupled synergistic anticancer drug has the general structural formula of (II). The drugs achieve the combined medication of various anticancer drugs and avoid the toxic reaction caused by the interaction among the drugs or by the pharmacokinetics of the drugs when taking various anticancer drugs, facilitate overcoming the multidrug resistance of cancers, have a synergistic effect, and can be used for preparing anticancer medicaments and for treating cancers.

Abstract: A method and kit for preparing a high-throughput sequencing library based on nested multiplex PCR are provided. The method includes: amplifying a targeted region using a forward primer and a reverse primer, where the reverse primer includes a reverse specific sequence at a 3?-end and a first universal sequencing sequence at a 5?-end; purifying; and amplifying the purified product using a nested primer located downstream of the forward primer, a first tag primer, and a second universal primer, where the nested primer includes a second universal sequencing sequence at a 5?-end and a specific sequence at a 3?-end, a 3?-end sequence of the first tag primer is partially or completely the same as the first universal sequencing sequence, the first tag primer further includes a first tag sequence, and a 3?-end sequence of the second universal primer is partially or completely the same as the second universal sequencing sequence.

Abstract: Provided are a method for constructing a sequencing library based on a DNA sample and use. The method includes: digesting the DNA sample with endonuclease to obtain a DNA sample with single-strand nicks; polymerizing the DNA sample with the single-strand nicks by using polymerase, dATP, dTTP, dGTP, and methylated dCTP to obtain a hybrid DNA, the hybrid DNA including two reversely complementary strands, where a 5?-end of each strand is an original sequence of the DNA sample, a 3?-end of each strand is a synthetic sequence, and all bases C in the 3?-end of each strand are methylated; subjecting the hybrid DNA to bisulfite treatment or other treatment to obtain converted hybrid DNA; and amplifying the converted hybrid DNA to obtain the sequencing library. Thus, the method can be used for whole genome bisulfite sequencing or multiplex PCR targeted sequencing and probe capture sequencing.

Abstract: A method and system for constructing sequencing library, includes: obtaining a transformed DNA sample with a universal sequence; performing amplification using a first specific primer located upstream of the target region and a first universal primer at least partially matching or overlapping the universal sequence; and performing amplification using a second specific primer, a second universal primer and a tagged primer. The second specific primer is located downstream of the first specific primer and upstream of the target region, the second universal primer overlaps at least a partial sequence of the second specific primer, and the tagged primer overlaps a partial sequence of the first universal primer. Alternatively, the second specific primer is located downstream of the target region, the second universal primer overlaps at least a partial sequence of the first specific primer, and the tagged primer overlaps a partial sequence of the second specific primer.

Abstract: Neuropeptide S receptor agonists are provided. The NPS agonists include trimeric, tetrameric, pentameric or hexameric peptidomimetic analogs exhibiting affinity for and activity at the neuropeptide S receptor. The peptidomimetic molecules may be useful in the treatment of disorders, syndromes and conditions mediated by modulation of the neuropeptide S receptor such as substance abuse, narcolepsy, insomnia, obesity, cognitive decline, dementia, Alzheimer's disease, panic disorder, generalized anxiety, PTSD, phobias, schizophrenia and as supportive medication during any kind of cessation program in cognitive behavioral therapy, such as drug addiction, eating disorders and gambling.

Abstract: An electronic package including modulated mesh planes can reduce crosstalk between adjacent signal wires. Modulated mesh planes above and below a wiring plane include sets of adjacent wires arranged parallel to signal wires within the wiring plane, and sets of adjacent wires arranged perpendicular to the signal wires. The wires in each of the mesh planes are electrically interconnected and insulated from the signal wires by a dielectric layer. The electronic package also includes a region of the mesh planes having the adjacent wires that are perpendicular to the signal wires separated by a first distance, and another region of the mesh planes having adjacent wires perpendicular to the signal wires separated by a distance greater than the first distance. A set of rectangular mesh areas of the mesh planes can be populated with supplemental wires and via interconnect structures which can further reduce crosstalk.