Abstract: A calibration method of a mixed mode simulation calibrates standard delay times in a standard delay format and includes obtaining a digital output circuit from a digital circuit, obtaining an analog output circuit from an analog circuit, performing a simulation on the digital output circuit connected to the analog output circuit to obtain an ideal output, obtaining a first delay time according to the standard delay times of the digital output circuit, performing a calibrative analog-to-digital mixed mode simulation using the first delay time to obtain an analog-to-digital mixed output, comparing the ideal output and the analog-to-digital mixed output to calibrate the first delay time, and calibrating the standard delay times of the digital output circuit according to the calibrated first delay time.