Abstract: A method is derived for identifying high affinity monoclonal antibody heavy and light chain pairs from high throughput screens of antibody producing cell libraries such as B-cell and hybridoma libraries. Specifically, the method relates to application of reversed immunocapture and high resolution tandem mass spectrometry for the identification of heavy and light chain pairs of binding antibodies obtained from high throughput screens of antibody producing cell libraries.

Abstract: A fiber amplifier is provided, including a pump laser (202), a pump and signal combiner (203), and a few-mode doped fiber (204). The pump laser (202) is configured to output pump light. The pump and signal combiner (203) is configured to couple input few-mode signal light and the pump light into the few-mode doped fiber (204). Refractive indexes of a fiber core of the few-mode doped fiber (204) are distributed to be gradient along a radial direction of a cross section, the fiber core is etched with periodic gratings along an axial direction, and periods of the gratings satisfy a phase matching condition. The fiber amplifier achieves strong coupling and co-amplification between optical signal modes, thereby reducing a differential gain between mode groups.

Abstract: A power module includes N groups of interleaved parallel PFC circuits and a controller. Each group of the interleaved parallel PFC circuits includes a PFC inductor, an autotransformer, a first rectifier bridge arm, and a second rectifier bridge arm. The autotransformer includes a first winding and a second winding that are coupled to each other. The controller is configured to: in response to that an absolute value of a difference between a current of the first winding and a current of the second winding is greater than or equal to a preset value, adjust a duty cycle of a drive signal of a switching transistor of the first rectifier bridge arm or the second rectifier bridge arm in a next switching cycle.

Abstract: A hardware resource mapping system and method; the system includes a host with a user space and external devices connected to the host, containing multiple hardware resources divided into M clusters; M is a positive integer; the user space includes n virtual functions and N virtual resources, with a first mapping relationship existing between them, where n and N are positive integers; a second mapping relationship exists between the N virtual resources and the M clusters, ensuring the number of virtual resources mapped to each cluster is greater than or equal to n, and the virtual resources mapped to each cluster correspond to the n virtual functions; for each cluster, a third mapping relationship exists between the virtual resources mapped to the cluster and the hardware resources included in the cluster, ensuring that each virtual resource is mapped to all hardware resources included in the cluster.

Abstract: Embodiments of an optical amplification apparatus are disclosed which include a first optical amplifier, a second optical amplifier, and a mode exchanger. The first optical amplifier is connected to an input port of the mode exchanger, and the second optical amplifier is connected to an output port of the mode exchanger. The first optical amplifier is configured to amplify optical signals carried in a plurality of transmission modes of a few-mode fiber, the plurality of transmission modes of the few-mode fiber may be grouped into N groups, each group includes two transmission modes, and N is a positive integer greater than or equal to 1. The mode exchanger is configured to exchange the two transmission modes carrying optical signals in each group. The second optical amplifier is configured to amplify the optical signals that are carried in the two transmission modes in each group and whose modes are exchanged.

Abstract: A system and method for identifying genomic regions with higher fragmentation rates than the local and global backgrounds as part of diagnosing early stage cancer is provided. The method includes steps of: de-novo characterizing genome-wide cell-free DNA fragmentation regions with higher fragmentation rates than the local and global backgrounds from whole-genome sequencing by weighing the fragment coverages in each region by a ratio of average fragment sizes in the region versus that in the whole chromosome to generate a score; and identifying DNA fragmentation regions of interest based upon comparing the score with a threshold. The system and method can utilize identified DNA fragmentation hotspots for the detection and localization of multiple early-stage cancers (or certain other non-malignant disease).

Abstract: The present invention provides a system and methods for detecting long-range cis-regulatory element (CRE) activities in a nucleic acid molecule obtained from a cell sample, the system comprising: one or more components for measuring multi-omics from a single nucleic acid molecule obtained from the cell sample, wherein the multi-omics measurements comprise one or more of: three-dimensional chromosomal conformation, CpG methylation, GpC accessibility, single nucleotide polymorphisms (SNPs); or one or more combinations thereof, and one or more components for measuring a transcriptome of the cell sample; wherein the system is capable of profiling a plurality of long-range CREs within the nucleic acid molecule obtained from the cell sample.

Abstract: An AC/DC converter and a charging apparatus. The AC/DC converter includes a circuit of at least one phase. In a circuit of each phase, a first end of an inductor is coupled to an alternating current power supply, and the second end of the inductor is coupled to a first end of a first winding and a first end of a second winding; the second end of the first winding is coupled to a winding connection end of a first switching unit, and the second end of the second winding is coupled to a winding connection end of a second switching unit; a bus connection end of the first switching unit is coupled to a bus of the AC/DC converter through at least two first rectifier units.

Abstract: A charging apparatus, a charging pile, and a charging system. The charging apparatus includes a positive bus, a negative bus, a DC/DC conversion circuit, and a soft-start circuit. The soft-start circuit is connected to an input end of the DC/DC conversion circuit through the positive bus and the negative bus, and the input end of the DC/DC conversion circuit is connected to a photovoltaic apparatus or an energy storage apparatus through the positive bus and the negative bus. Therefore, when a first voltage between the positive bus and the negative bus is greater than or equal to a first threshold, the DC/DC conversion circuit may perform power conversion on a second voltage received from the photovoltaic apparatus or the energy storage apparatus, and output the second voltage obtained after power conversion.

Abstract: As described below, disclosed herein are methods of analyzing DNA methylation in cell-free DNA (cfDNA) and genomic DNA (gDNA) from sequencing data.

Abstract: A detection circuit includes a detection power supply and a digital unit that are connected in series. A first end and a second end of the detection circuit are respectively connected to an input end and an output end of an anti-backflow circuit. The detection power supply may make an electric potential at the second end of the detection circuit higher than that at the first end of the detection circuit. When transmitting a detection current, the digital unit may output a first level signal indicating that the anti-backflow circuit fails. The detection current is a current transmitted when a loop is formed between the first end and the second end of the detection circuit.

Abstract: As described below, disclosed herein are methods of analyzing DNA methylation in cell-free DNA (cfDNA) and genomic DNA (gDNA) from sequencing data.

Abstract: Embodiments of an optical amplification apparatus are disclosed which include a first optical amplifier, a second optical amplifier, and a mode exchanger. The first optical amplifier is connected to an input port of the mode exchanger, and the second optical amplifier is connected to an output port of the mode exchanger. The first optical amplifier is configured to amplify optical signals carried in a plurality of transmission modes of a few-mode fiber, the plurality of transmission modes of the few-mode fiber may be grouped into N groups, each group includes two transmission modes, and N is a positive integer greater than or equal to 1. The mode exchanger is configured to exchange the two transmission modes carrying optical signals in each group. The second optical amplifier is configured to amplify the optical signals that are carried in the two transmission modes in each group and whose modes are exchanged.

Abstract: The present invention belongs to the field of plant biotechnology engineering, and particularly relates to a method for improving flavonoid phenylpropanoid compounds in a Saussurea involucrata cell culture. In a medium used in the method, a phosphorus concentration is 0.5-3 mmol/L, a NO3? ion concentration is 20-35 mmol/L, a NH4+ ion concentration is 0-30 mmol/L, a Ca2+ ion concentration is 0.5-3 mmol/L, a Mg2+ ion concentration is 0.2-1.5 mmol/L, and a boron ion concentration is 0.02-0.1 mmol/L; an inducer or precursor or bypass metabolism inhibitor may be added to the medium. Finally, the content of total flavonoids and the contents of rutin, chlorogenic acid, syringin and 1,5-dicaffeoylquinic acid in the Saussurea involucrata cell culture are significantly improved.

Abstract: There is provided an air pressure system for controlling an air compressor in real time in accordance with the actual usage of compressed air by a plurality of terminals. Furthermore, in case pressure losses change abruptly, unwanted electric power is prevented from being consumed by a stable operation free of response delays on the basis of a predicted model that assesses time lags of volume responses.

Abstract: A method for certificate transaction validation of a blockchain-based resource public key infrastructure aims to avoid security threats caused by conflicts or illegal transactions during transactions and improve security. A technical solution is as follows: constructing a resource public key infrastructure RPKIB system composed of a resource issuer, a resource transaction application client, a resource receiver, a blockchain network and a validation node; designing operations of a resource certificate and route origin authorization ROA as transactions, submitting operations of various resource transactions initiated by a resource issuer as transactions to the blockchain network, running, by the validation node, a smart contract to verify the transactions, and distinguishing whether the operations are malicious behaviors or normal operations of an authority.

Abstract: A fiber amplifier is provided, including a pump laser (202), a pump and signal combiner (203), and a few-mode doped fiber (204). The pump laser (202) is configured to output pump light. The pump and signal combiner (203) is configured to couple input few-mode signal light and the pump light into the few-mode doped fiber (204). Refractive indexes of a fiber core of the few-mode doped fiber (204) are distributed to be gradient along a radial direction of a cross section, the fiber core is etched with periodic gratings along an axial direction, and periods of the gratings satisfy a phase matching condition. The fiber amplifier achieves strong coupling and co-amplification between optical signal modes, thereby reducing a differential gain between mode groups.

Abstract: The disclosure discloses a method for bidirectional authorization of a blockchain-based resource public key infrastructure, aiming at solving security threat problems that a legal BGP route is illegal and a legal IP address is blocked caused by malicious operations.

Abstract: The present disclosure provides a method for increasing lycopene content in a tomato fruit, including knocking out the gene LIE1 of SEQ ID No 1. The disclosure also provides a method for knocking out gene LIE1 in tomato. The method of the disclosure is effective for increasing the content of lycopene in tomato fruits. Finally, the disclosure provides a transgenic tomato plant with knockout of gene LIE1.

Abstract: A method is described for identifying high affinity monoclonal antibody heavy and light chain pairs from high throughput screens of antibody producing cell libraries such as B-cell and hybridoma libraries. Specifically, the method relates to application of reversed immunocapture and high resolution tandem mass spectrometry for the identification of heavy and light chain pairs of binding antibodies obtained from high throughput screens of antibody producing cell libraries.