Abstract: The present invention provides a nitrilase mutant protein with increased thermal stability and its application in the synthesis of an anti-epileptic drug intermediate, wherein the mutant is obtained by mutating one or two of the amino acids at position 151, 223 and 250 of the amino acid sequence shown in SEQ ID No. 2. the thermal stability of the nitrilase mutant AcN-T151V/C223A/C250G was increased by up to 1.73 folds. The yield of the final product was up to 95% using the recombinant Escherichia coli containing the nitrilase mutant to hydrolyze 1M 1-cyanocyclohexylacetonitrile to produce 1-cyanocyclohexyl acetic acid at 35° C. And the yield of the final product was up to 97% when hydrolyzing 1.2M 1-cyanocyclohexylacetonitrile at 35° C. The final yield was up to 80% when using the nitrilase mutants obtained by the present invention to synthesize gabapentin.

Abstract: A charging apparatus, a charging pile, and a charging system. The charging apparatus includes a positive bus, a negative bus, a DC/DC conversion circuit, and a soft-start circuit. The soft-start circuit is connected to an input end of the DC/DC conversion circuit through the positive bus and the negative bus, and the input end of the DC/DC conversion circuit is connected to a photovoltaic apparatus or an energy storage apparatus through the positive bus and the negative bus. Therefore, when a first voltage between the positive bus and the negative bus is greater than or equal to a first threshold, the DC/DC conversion circuit may perform power conversion on a second voltage received from the photovoltaic apparatus or the energy storage apparatus, and output the second voltage obtained after power conversion.

Abstract: A method and an apparatus for compensating for wavelength drift are disclosed. The method includes: generating, by a burst control circuit, a burst bias current; sending, by the burst control circuit, the burst bias current to a light emitting part and a trigger; converting, by the trigger, the received burst bias current into burst DA data; sending, by the trigger, the burst DA data to a synthesizer circuit; receiving, by the synthesizer circuit, the burst DA data sent by the trigger and the calibrated DA data sent by the MCU respectively; synthesizing, by the synthesizer circuit, the burst DA data and the calibrated DA data to obtain a synthesized signal; and sending, by the synthesizer circuit, the synthesized signal to a temperature control part.

Abstract: A method and an apparatus for compensating for wavelength drift are disclosed. The method includes: generating, by a burst control circuit, a burst bias current; sending, by the burst control circuit, the burst bias current to a light emitting part and a trigger; converting, by the trigger, the received burst bias current into burst DA data; sending, by the trigger, the burst DA data to a synthesizer circuit; receiving, by the synthesizer circuit, the burst DA data sent by the trigger and the calibrated DA data sent by the MCU respectively; synthesizing, by the synthesizer circuit, the burst DA data and the calibrated DA data to obtain a synthesized signal; and sending, by the synthesizer circuit, the synthesized signal to a temperature control part.