Abstract: Disclosed are a gene mining method combining functional sequence and structure simulation, an NADH-preferring phosphinothricin dehydrogenase mutant and an application thereof. The gene mining method comprises the following steps: (1) analyzing a characteristic sequence which an NADH-type glutamate dehydrogenase should have; (2) searching a gene library based on the characteristic sequence; (3) performing clustering analysis and protein structure simulation on genes obtained by the searching; (4) selecting genes that feature high gene aggregation and a protein structure similar to that of the known phosphinothricin dehydrogenase as candidate genes. A wild-type phosphinothricin dehydrogenase with an amino acid sequence as set forth in SEQ ID No.

Abstract: The present disclosure provides a fault location method, a fault location system, and a video management system, and relates to the technical field of network communication. The fault location method includes: determining, in response to a case where it is determined that a target video from a video acquisition system has a fault, a network identifier of a target camera corresponding to the target video; extracting target alarm data corresponding to the network identifier from current alarm data of the video acquisition system according to the network identifier of the target camera; and determining a fault cause of the video acquisition system according to a preset corresponding relationship between fault events and alarm information, and the target alarm data. The present disclosure can be used for centralized management and operation and maintenance of devices and systems related to video services.