Abstract: Disclosed is a device of automatically detecting nucleic acid, including: a fixing frame; a cartridge positioning assembly having a cartridge loading position configured to position a cartridge; a lifting assembly including a lifting base configured to be controllably moved toward or away from the cartridge positioning assembly in a first direction; a rotating assembly including a rotating member capable of controllably rotating relative to the lifting base around a rotation axis parallel to the first direction; and a liquid transferring assembly including a connecting portion configured to connect to a tip. The liquid transferring assembly is capable of rotating with the rotating member to a position where the connecting portion is aligned with any liquid storing tank of the cartridge in the first direction, the liquid transferring assembly is capable of moving along with the lifting base to an inserting position or a disengaging position along the first direction.

Abstract: The present invention provides a nitrilase mutant protein with increased thermal stability and its application in the synthesis of an anti-epileptic drug intermediate, wherein the mutant is obtained by mutating one or two of the amino acids at position 151, 223 and 250 of the amino acid sequence shown in SEQ ID No. 2. the thermal stability of the nitrilase mutant AcN-T151V/C223A/C250G was increased by up to 1.73 folds. The yield of the final product was up to 95% using the recombinant Escherichia coli containing the nitrilase mutant to hydrolyze 1M 1-cyanocyclohexylacetonitrile to produce 1-cyanocyclohexyl acetic acid at 35° C. And the yield of the final product was up to 97% when hydrolyzing 1.2M 1-cyanocyclohexylacetonitrile at 35° C. The final yield was up to 80% when using the nitrilase mutants obtained by the present invention to synthesize gabapentin.

Abstract: Disclosed are a gene mining method combining functional sequence and structure simulation, an NADH-preferring phosphinothricin dehydrogenase mutant and an application thereof. The gene mining method comprises the following steps: (1) analyzing a characteristic sequence which an NADH-type glutamate dehydrogenase should have; (2) searching a gene library based on the characteristic sequence; (3) performing clustering analysis and protein structure simulation on genes obtained by the searching; (4) selecting genes that feature high gene aggregation and a protein structure similar to that of the known phosphinothricin dehydrogenase as candidate genes. A wild-type phosphinothricin dehydrogenase with an amino acid sequence as set forth in SEQ ID No.

Abstract: The present invention provides an embedded-outer rotor motor direct driven mower driving wheel and a mower. The embedded-outer rotor motor direct driven mower driving wheel comprises a driving wheel main body formed by the assembling of a wheel side cover I, a wheel side cover II, and a wheel body sandwiched between the wheel side cover I and the wheel side cover II, the wheel side cover I, the wheel side cover II and the wheel body are enclosed to define a cavity, an outer rotor motor is mounted in the cavity, an outer rotor portion of the outer rotor motor is fixedly arranged relative to the driving wheel main body, an internal stator portion of the outer rotor motor is provided with a fixed shaft penetrating through the wheel side cover I and extending out of the driving wheel main body, and the wheel side cover I is provided with a shaft hole I allowing the fixed shaft to penetrate through.

Abstract: The present invention provides an embedded-outer rotor motor direct driven mower driving wheel and a mower. The embedded-outer rotor motor direct driven mower driving wheel comprises a driving wheel main body formed by the assembling of a wheel side cover I, a wheel side cover II, and a wheel body sandwiched between the wheel side cover I and the wheel side cover II, the wheel side cover I, the wheel side cover II and the wheel body are enclosed to define a cavity, an outer rotor motor is mounted in the cavity, an outer rotor portion of the outer rotor motor is fixedly arranged relative to the driving wheel main body, an internal stator portion of the outer rotor motor is provided with a fixed shaft penetrating through the wheel side cover I and extending out of the driving wheel main body, and the wheel side cover I is provided with a shaft hole I allowing the fixed shaft to penetrate through.