Abstract: A method for asymmetrically preparing L-phosphinothricin by oxidation-reduction reaction through biological multienzyme coupling, where D,L-phosphinothricin as a raw material is catalyzed by an enzyme catalysis system to obtain L-phosphinothricin, wherein the enzyme catalysis system comprises a D-amino acid oxidase mutant for catalyzing D-phosphinothricin in D,L-phosphinothricin into 2-carbonyl-4-[hydroxy(methyl)phosphono] butyric acid and a transaminase for catalytic reduction of the 2-carbonyl-4-[hydroxy(methyl)phosphono] butyric acid into L-phosphinothricin; the D-amino acid oxidase mutant is obtained by mutation of D-amino acid oxidase in wild strain Rhodotorula taiwanensis at one of the following sites: (1) M213S-N54V-F58E; (2) M213S-N54V-F58E-D207A; (3) M213S-N54V-F58E-D207A-S60T.

Abstract: A method for chemical-biological cascade synthesis of L-phosphinothricin is carried out as follows: 3-(methylethoxyphosphinyl) ethyl propionate is synthesized by addition reaction from diethoxymethylphosphine and acrylic acid, then a condensation reaction is carried out with 3-(methylethoxyphosphinyl) ethyl propionate and sodium ethoxide as reactants, then the product is subjected to a hydrolysis reaction with diethyl oxalate to synthesize 4-(hydroxymethylphosphinyl)-2-oxobutyric acid, and finally, L-phosphinothricin is catalytically synthesized by taking 4-(hydroxymethylphosphinyl)-2-oxobutyric acid as a raw material, and using highly active and stable wet cells co-expressing phsophinothricin dehydrogenase and alcohol dehydrogenase or co-expressing a phsophinothricin dehydrogenase mutant and alcohol dehydrogenase as a biocatalyst, thereby solving the problems of existing L-phosphinothricin synthesis being tedious, low asymmetric amination reduction activity and poor stability.

Abstract: The present invention provides an embedded-outer rotor motor direct driven mower driving wheel and a mower. The embedded-outer rotor motor direct driven mower driving wheel comprises a driving wheel main body formed by the assembling of a wheel side cover I, a wheel side cover II, and a wheel body sandwiched between the wheel side cover I and the wheel side cover II, the wheel side cover I, the wheel side cover II and the wheel body are enclosed to define a cavity, an outer rotor motor is mounted in the cavity, an outer rotor portion of the outer rotor motor is fixedly arranged relative to the driving wheel main body, an internal stator portion of the outer rotor motor is provided with a fixed shaft penetrating through the wheel side cover I and extending out of the driving wheel main body, and the wheel side cover I is provided with a shaft hole I allowing the fixed shaft to penetrate through.

Abstract: The present invention provides an embedded-outer rotor motor direct driven mower driving wheel and a mower. The embedded-outer rotor motor direct driven mower driving wheel comprises a driving wheel main body formed by the assembling of a wheel side cover I, a wheel side cover II, and a wheel body sandwiched between the wheel side cover I and the wheel side cover II, the wheel side cover I, the wheel side cover II and the wheel body are enclosed to define a cavity, an outer rotor motor is mounted in the cavity, an outer rotor portion of the outer rotor motor is fixedly arranged relative to the driving wheel main body, an internal stator portion of the outer rotor motor is provided with a fixed shaft penetrating through the wheel side cover I and extending out of the driving wheel main body, and the wheel side cover I is provided with a shaft hole I allowing the fixed shaft to penetrate through.