Abstract: Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and/or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein/polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.

Abstract: Devices, methods and kits for rapid and simple determination of target molecules, including small molecules, polypeptides, proteins, cells and infectious disease agents in liquid samples that are capable of real-time measurement of these entities in fluid samples that are highly selective, highly sensitive, simple to operate, low cost, and portable. The devices, methods and kits also provide, in at least some embodiments, the use of MIPs in a flow through or lateral flow device.

Abstract: Devices, methods and kits for rapid and simple determination of target molecules, including small molecules, polypeptides, proteins, cells and infectious disease agents in liquid samples that are capable of real-time measurement of these entities in fluid samples that are highly selective, highly sensitive, simple to operate, low cost, and portable. The devices, methods and kits also provide, in at least some embodiments, the use of MIPs in a flow through or lateral flow device.

Abstract: Liquid discharge apparatus, particularly useful as a portable inoculation gun for anti-virus inoculation of plants, includes a housing having a handle at one end configured for convenient grasping by a user, a nozzle at the opposite end for discharging liquid from a discharge opening therein, and a maniputable member manually actuatable to produce a discharge of liquid from the nozzle. The nozzle includes a gas passageway communicating with the discharge opening, and a liquid passageway communicating with the gas passageway. A gas delivery system is actuatable by the maniputable member to discharge gas at high-velocity through the discharge opening via the gas passageway. A liquid source communicates with the liquid passageway in the nozzle such that each high-velocity discharge of gas from the nozzle via the gas passageway draws a quantity of liquid from the liquid source into the gas passageway and discharges it at high-velocity with the gas through the discharge opening.

Abstract: Liquid discharge apparatus, particularly useful as a portable inoculation gun for anti-virus inoculation of plants, includes a housing having a handle at one end configured for convenient grasping by a user, a nozzle at the opposite end for discharging liquid from a discharge opening therein, and a maniputable member manually actuatable to produce a discharge of liquid from the nozzle. The nozzle includes a gas passageway communicating with the discharge opening, and a liquid passageway communicating with the gas passageway. A gas delivery system is actuatable by the maniputable member to discharge gas at high-velocity through the discharge opening via the gas passageway. A liquid source communicates with the liquid passageway in the nozzle such that each high-velocity discharge of gas from the nozzle via the gas passageway draws a quantity of liquid from the liquid source into the gas passageway and discharges it at high-velocity with the gas through the discharge opening.