Abstract: A microneedle patch for enhancing accumulation of PpIX in a solid tumor and a preparation method therefor. The method includes steps of: dissolving albumin into Dubos medium, taking the albumin as a template, adding CuCl2 and CaCl2 for mineralization to obtain CCP nanoparticles; loading CAT and 5-ALA onto a surface of the CCP nanoparticles by a one-step carbodiimide coupling method and an electrostatic-adsorption method, respectively, to obtain CCPCA nanoparticles; loading the CCPCA nanoparticles and microneedle-matrix solution into a microneedle mold, after vacuum drying and demolding, obtaining the microneedle patch for enhancing accumulation of PpIX in a solid tumor.

Abstract: The present disclosure provides a preparation method of a yak hide-derived oligopeptide ferrous chelate with a high antioxidant activity. The preparation method includes preparing a yak hide-derived oligomeric collagen peptide and subjecting the yak hide-derived oligomeric collagen peptide as a protein source to chelation with an iron source in water. A pretreated yak hide is subjected to enzymatic hydrolysis under a pH value of 7 at 50° C. for 4 h with an amount of an enzyme added at 2% to obtain a yak skin-derived collagen with a molecular weight of less than 2 kDa; the chelation is conducted in a peptide-to-iron mass ratio of 1:1 to 5:1 with a peptide concentration of 1% to 5% at 30° C. to 70° C. for 20 min to 60 min under a pH value of 3 to 8; and an iron chelating capacity is 42.72 mg/g under optimal preparation conditions.