Abstract: Disclosed are constructs and methods to accelerate maturation of human pluripotent stem cell derived cardiomyocytes by maintaining them on a Cardiac Mimetic Matrix (CMM) substrate.

Abstract: A computer-implemented method and corresponding system provide orthodontic treatment options for use in orthodontic diagnosis or treatment planning. The method performs a rules-based expert system analysis on a given feature variable to produce expert system treatment options. The given feature variable represents an orthodontic feature of a patient. The method applies a computer-implemented multi-component model to a given set of feature variables to produce multi-component model-based treatment options that include primary and secondary model-based treatment options. The method compares the expert system treatment options to the multi-component model-based treatment options to determine disagreement or agreement between each other, enabling a suitable treatment decision to be arrived at which can be valuable to clinicians for verifying treatment plans, minimizing human error, training orthodontists, and improving reliability.

Abstract: The present invention relates to the unexpected discovery of methods and devices that can be used for high-throughput precise quantification, detection and/or temporal profiling of cellular secretions. In various embodiments, the methods of the invention allow for high-throughput absolute detection of secretions of cells, identification of the nature of the secreted molecules, and/or the nature of the secreting cells. Further, the present invention includes a device combining microfluidics and antibody printing, wherein the device can be used to detect protein secretion signature of cells in a high-throughput manner. Further, the present invention includes compositions comprising molecules that can be used to reduce cell death and to implement cell-less therapies. Further, the present invention includes a method for training an algorithm to predict temporal profile of cellular secretion.