Abstract: The present invention provides a protein having a binding capacity to a plasticizer, which has been conferred with useful properties for measuring, quantifying, or concentrating a plasticizer, such as high sensitivity, low cross-reactivity, high tolerance for interferents, and high tolerance for solvents. Specifically, the present invention provides a modified protein having various properties, such as affinity for antigenic plasticizers, antigen binding capacity, cross-reactivity, tolerance for antigen-antibody reaction interferents, tolerance for enzymatic color developing reaction interferents, and tolerance for solvents, improved by gene recombination technology.

Abstract: The present invention provides for a method of selecting an antibody against a target substance to be measured, which comprises selecting the antibody against the target substance by antigen-antibody reaction in the presence of a substance interfering with the antigen-antibody reaction. That is, an antigen and a labeled antigen are reacted with the antibody in the presence of an interfering substance, such as an environment pollutant, and on the basis of the degree of reaction thereof, the antibody against the target substance, which is highly resistant to the interfering substance, is selected. Thereby, even if a test sample is contaminated with a substance interfering with antigen-antibody reaction, the antibody, which is highly resistant to the substance interfering with antigen-antibody reaction, is not influenced by the interfering substance and gives a correct value in the quantification.

Abstract: The present invention relates to an isolation material for an endocrine disrupter, which is prepared by immobilizing an anti-endocrine disrupter antibody to a graft-chain-containing carrier made of a polymeric material via covalent binding. According to the present invention, an isolation material for an endocrine disrupter, which is capable of specific and efficient isolation of an endocrine disruptor, and an endocrine disrupter concentration and a clean-up method capable of specific and efficient concentration or clean-up of an endocrine disruptor at a high rate can be afforded.

Abstract: The present invention provides a protein capable of binding to a female sex hormone, which protein is supplemented with useful properties for measuring, quantifying and concentrating female sex hormones, such as high sensitivity, low cross-reactivity, unlikelihood of being influenced by interfering substances, and unlikelihood of being influenced by solvents. Specifically, the present invention provides a recombinant protein prepared by obtaining various genes for various antibodies against female sex hormones, and modifying, by gene recombination technology, various properties of the original antibody, such as affinity, avidity, cross-reactivity, resistance for interfering substance on antigen-antibody reaction, resistance for interfering substance on enzymatic color developing reaction, resistance for solvent and the like.

Abstract: On a surface of a well previously disposed on a plate, a primary antibody that recognizes vitellogenin is solid-phased, in the well a sample obtained from a test body exposed to an environment is injected to react, followed by injecting a secondary antibody that is labeled with an enzyme and recognizes the vitellogenin, further followed by injecting a chromogenic reagent to cause a coloring reaction and by measuring the stained amount, still further followed by calculating an amount of vitellogenin from the stained amount to evaluate an environment based on the amount of vitellogenin.

Abstract: The present invention provides a protein capable of binding to an environmental hormone, which protein is supplemented with useful properties for measuring, quantifying and concentrating environmental hormones, such as high sensitivity, low cross-reactivity, unlikelihood of being influenced by interfering substances, and unlikelihood of being influenced by solvents. Specifically, the present invention provides a modified protein prepared by obtaining various genes for various antibodies with environmental hormones, and modifying, by gene recombination technology, various properties of the original antibody, such as affinity, avidity, cross-reactivity, resistance for interfering substance on antigen-antibody reaction, resistance for interfering substance on enzymatic color developing reaction, resistance for solvent and the like.

Abstract: A hybridoma which produces a monoclonal antibody against an endocrine disruptor or its degradation product obtained by fusing spleen cells or lymphoid cells of an animal having been immunized with a complex of the endocrine disruptor or a compound similar thereto with a protein with myeloma cells; the monoclonal antibody produced thereby; and a method for immunologically detecting the endocrine disruptor or its degradation product and a method for immunologically concentrating the same each by using the above antibody.

Abstract: The present invention provides a method of selecting an antibody against a target substance to be measured which comprises selecting the antibody against the target substance by antigen-antibody reaction in the presence of a substance interfering with the antigen-antibody reaction.

Abstract: A hybridoma obtainable by fusing a spleen cell or lymphocyte of an animal immunized by a complex of a surfactant compound for synthetic detergent and protein with a myeloma cell, which produces a monoclonal antibody against the compound or its degradation product. A monoclonal antibody against a surfactant compound for synthetic detergent or its degradation product which is produced by the hybridoma, a kit for immunoassay of detergent, its degradation product or a mixture thereof containing as an essential constitutional component the monoclonal antibody, and an immunoassay method, in particular, ELISA of detergent, its degradation product or a mixture thereof in a specimen by reacting the specimen with the monoclonal antibody supported on a carrier and the detergent, its degradation product or the mixture thereof labeled with a labeling agent are also disclosed.

Abstract: A microorganism fertilizer which causes in the soil a phenomenal multiplication of fluorescent Pseudomonas that have a superior effect in preventing the common scab of potato. The microorganism fertilizer is obtained such that medium-temperature and high-temperature Actinomycetes are used as seed bacteria and cultured while being spread/agitated in an organic substance such as livestock manure, etc. in an isolated propagation bed. The thus obtained microorganism fertilizer can be used as a countermeasure against environmental pollution.