Abstract: The present invention provides a 2-OST mutant exhibiting a high activity.

Abstract: The present invention provides a method for producing a heparosan compound having an isomerized hexuronic acid residue and a method for producing a heparan sulfate with improved C5-epimerization efficiency.

Abstract: The present invention provides a 2-OST mutant exhibiting a high activity.

Abstract: Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.

Abstract: Ligase mutants of the following (1), (2), or (3): (1) a ligase mutant comprising an amino acid sequence showing 95% or more identity to the amino acid sequence of SEQ ID NO: 1, and having a nucleic acid-linking activity; (2) a ligase mutant comprising an amino acid sequence showing 90% or more identity to the amino acid sequence of SEQ ID NO: 2, and having a nucleic acid-linking activity; or (3) a ligase mutant comprising an amino acid sequence showing 97% or more identity to the amino acid sequence of SEQ ID NO: 3, and having a nucleic acid-linking activity, have excellent properties.

Abstract: Ligase mutants of the following (1), (2), or (3): (1) a ligase mutant comprising an amino acid sequence showing 95% or more identity to the amino acid sequence of SEQ ID NO: 1, and having a nucleic acid-linking activity; (2) a ligase mutant comprising an amino acid sequence showing 90% or more identity to the amino acid sequence of SEQ ID NO: 2, and having a nucleic acid-linking activity; or (3) a ligase mutant comprising an amino acid sequence showing 97% or more identity to the amino acid sequence of SEQ ID NO: 3, and having a nucleic acid-linking activity, have excellent properties.

Abstract: Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.

Abstract: The present invention provides a novel sulfated polysaccharide having anticoagulant activity. Specifically, the present invention provides a polysaccharide that includes a repetitive structure of a disaccharide unit composed of a hexuronic acid (HexA) residue and a ?-D-glucosamine (GlcN) residue and having 13% or higher of a 3-O-sulfation rate in GlcN residues.

Abstract: Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.

Abstract: The present invention provides a 2-OST mutant exhibiting a high activity.

Abstract: The present invention provides a method for producing a heparosan compound having an isomerized hexuronic acid residue and a method for producing a heparan sulfate with improved C5-epimerization efficiency.

Abstract: The present invention provides methods for producing 1,5-pentamethylenediamine (“1,5-PD”) efficiently in a manner suitable for an actual production. Specifically, the present invention provides a method of producing 1,5-pentamethylenediamine including allowing a lysine decarboxylase mutant to act on L-lysine and/or a salt thereof, wherein said lysine decarboxylase mutant has an amino acid sequence selected from the group consisting of: (a) the amino acid sequence of SEQ ID NO:1, (b) an amino acid sequence of SEQ ID NO: 1, but having one or several amino acid residue substitutions, deletions, insertions or additions, and (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:1, and having a lysine decarboxylation activity, and wherein said lysine decarboxylase has improved thermal stability.

Abstract: Hemicellulase that degrades corn non-starch polysaccharides (“NSP”), DNA encoding the same, and a method of using the hemicellulase and its DNA are provided. Proteins having hemicellulase activity such as Xyn5A, Xyn10B, Xyn11A, Xyn30A, and Xyn43A are described.

Abstract: The present invention provides a novel sulfated polysaccharide having anticoagulant activity. Specifically, the present invention provides a polysaccharide that includes a repetitive structure of a disaccharide unit composed of a hexuronic acid (HexA) residue and a ?-D-glucosamine (GlcN) residue and having 13% or higher of a 3-O-sulfation rate in GlcN residues.

Abstract: The present invention provides methods for producing 1,5-pentamethylenediamine (“1,5-PD”) efficiently in a manner suitable for an actual production. Specifically, the present invention provides a method of producing 1,5-pentamethylenediamine including allowing a lysine decarboxylase mutant to act on L-lysine and/or a salt thereof, wherein said lysine decarboxylase mutant has an amino acid sequence selected from the group consisting of: (a) the amino acid sequence of SEQ ID NO:1, (b) an amino acid sequence of SEQ ID NO: 1, but having one or several amino acid residue substitutions, deletions, insertions or additions, and (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:1, and having a lysine decarboxylation activity, and wherein said lysine decarboxylase has improved thermal stability.

Abstract: The present invention provides a method for producing the peptides comprising: cultivating in medium the microorganisms wherein at least one gene selected from the group consisting of a gene encoding; aminoacylhistidine peptidase; a gene encoding leucylaminopeptidase; and a gene encoding isoaspartyldipeptidase, respectively; has been disrupted on the chromosome and wherein preferably transformed with the recombinant DNA, comprising polynucleotide encoding the proteins having peptide-synthesizing activity, mixing at least one of the cultivated microorganisms and the disrupted cells of the microorganisms with the carboxy and amine components for the peptide synthesis.

Abstract: There is provided a mutant nucleoside-5?-phosphate producing enzyme with improved nucleoside-5?-phosphate producing ability of a nucleoside-5?-phosphate producing enzyme that has transphosphorylation activity and/or phosphatase activity and has one Lys residue, two Arg residues and two His residues with distances between their C?'s within a particular ranges and a space around them allowing a binding of a nucleoside, of which mutation is designed by using the crystal structure of EB-AP.

Abstract: A highly expressible recombinant polynucleotide is constructed. The recombinant polynucleotide contains a nucleotide sequence encoding a signal peptide derived from the N-terminal region of acid phosphatase present in bacterium belonging to Enterobacteriaceae. In addition the recombinant polynucleotide may contain a nucleotide sequence encoding a protein integrated downstream of the nucleotide sequence encoding the signal peptide. A transformant is constructed by introducing the recombinant polynucleotide to produce the protein.

Abstract: The present invention provides a method for producing the peptides comprising: cultivating in medium the microorganisms wherein at least one gene selected from the group consisting of a gene encoding; aminoacylhistidine peptidase; a gene encoding leucylaminopeptidase; and a gene encoding isoaspartyldipeptidase, respectively; has been disrupted on the chromosome and wherein preferably transformed with the recombinant DNA, comprising polynucleotide encoding the proteins having peptide-synthesizing activity, mixing at least one of the cultivated microorganisms and the disrupted cells of the microorganisms with the carboxy and amine components for the peptide synthesis.

Abstract: A protein which has a GMP synthetase activity, a DNA which codes for the protein and a transformant which is introduced with the DNA in a form that allows expression of the protein encoded by the DNA.