Abstract: [Problems] To provide a novel use of multipotential stem cells originating in a fat tissue. [Means for Solving Problems] It is intended to provide a cell preparation which contains multipotential stem cells originating in a fat tissue and is usable for an ischemic disease, impairment of renal function, wound, urinary incontinence or osteoporosis. As the multipotential stem cells originating in a fat tissue, use is made of cells which proliferate in the case of centrifuging cells separated from a fat tissue and culturing the thus sedimented cells (an SVF fraction) under low-serum conditions. In an embodiment, a cell preparation containing the SVF fraction is provided.

Abstract: It is intended to provide a pluripotent cell having the following properties: (1) being contained in a mixed-cell type population obtained by enzymatic treatment of the tissue collected from an animal; (2) being contained in a sedimented cell population obtained by centrifugating the mixed-cell type population of the property (1); (3) selectively proliferating by culturing in a medium containing 2% (v/v) or lower serum and 1 to 100 ng/ml of fibroblast growth factor-2; and (4) being differentiated into cells having the characteristics of adipocytes, osteoblasts, chondrocytes, tendon cells, myocardial cells, myoblasts, neurocytes or vascular endothelial cells by adjusting the culture condition; cells having differentiated from the above cell; and a method of conveniently obtaining the pluripotent cell in a large amount.