Abstract: A pump unit includes a pump and a metal ion trap. The pump includes a metal member that comes into contact with a mobile phase. The pump pumps a mobile phase through a flow path. The metal ion trap includes a filter element. The filter element includes a metal ion retention structure for retaining metal ions by interacting with the metal ions included in a mobile phase. The filter element is provided in the flow path for a mobile phase pumped by the pump. The pump unit may be provided in a chromatograph.

Abstract: In order to appropriately set MSm analysis conditions, an MSm-1 analysis executer (51) makes a mass spectrometer (20) perform an MSm-1 analysis (where m is an integer from 2 to n) to acquire three-dimensional data showing an intensity for each of the N m/z values and each of the M retention times (where N and M are natural numbers). Based on the three-dimensional data, a data matrix creator (41) creates data matrix X in which intensity data are arranged in N rows which differ from each other in m/z value and M columns which differ from each other in retention-time value. A matrix factorization executer (42) determines an N×K spectrum matrix S and K×M profile matrix P (where K is a natural number) by matrix factorization based on data matrix X so that this matrix X is approximated by product SP of the matrices S and P. An m/z detector (43) detects m/z of a precursor ion originating from a sample component from the values of the matrix elements in each column of matrix S.

Abstract: Provided is a method for determining a first N×K matrix S and second K×M matrix P, using factor number K, so that their product SP approximates to an N×M data matrix X obtained by analyzing a sample containing an unknown number of components. Multiple candidates of regularization parameter ?r and one sparsity-inducing regularization function R(S,P) are prepared. For each regularization-parameter candidate ?r, a candidate Sr of matrix S and candidate Pr of matrix P which minimize a loss function L(S,P)=D(X|SP)+?rR(S,P) are determined, where D(X|SP) is a distance function expressing the degree of difference between X and SP. For each combination of matrix element Xnm in X and corresponding matrix element (SrPr)nm in SrPr, a transformed value ynm=Fnm(Xnm(SrPr)nm) is determined using function Fnm which performs variable transform from probability distribution Pnm corresponding to D(Xnm|(SP)nm) into common probability distribution Pcommon, and goodness of fit between ynm and Pcommon is calculated.

Abstract: Provided is a method for identifying proteins capable of increasing the number of identified proteins contained in a target sample derived from blood. A protein contained in the target sample derived from blood is fragmented, and a protein contained in an having less bias in a quantity ratio of proteins than the target sample is fragmented, and the fragmented proteins are mixed (Steps S101 to S103). In this manner, the mixed sample in which the bias in a quantity ratio of proteins is less than that of the target sample is generated. By performing MS/MS measurement using the generated mixed sample (Step S107), an MS/MS spectrum of a peak derived from a protein contained in a small amount in the target sample can be prevented from being missed. Accordingly, the number of identified proteins contained in the target sample derived from blood can be increased.

Abstract: A inner-wall-coated capillary in which a zwitterionic polymer including a phosphate group including, for example, a phosphorylcholine group, is fixed on a wall surface including, for example, silanol by ionic interaction, is obtained by flowing a polymer solution including a phospholipid polymer combining, for example, MPC and BMA or MPC and SMA, through a capillary that includes silanol on the wall surface, for example. Consequently, adsorption of phosphorylated compounds can be prevented simply and highly durably.

Abstract: The present inventor has established protein abundance index (PAI, ?) to determine the protein contents in a protein mixture solution using nanoLC-MSMS data. Digested peptides were analyzed by nanoLC-MS/MS and the obtained results were applied to a Mascot protein identification algorism based on tandem mass spectra. For absolute quantitation, PAI was converted to exponentially modified PAI (EMPAI, m ?), which is proportional to protein contents in the protein mixture. EMPAI was successfully applied to comprehensive protein expression analysis and performed a comparison study between gene and protein expression in an HCT116 human cancer cells. Accordingly, the present invention provides a method and a computer program for quantifying the protein contents based on the protein abundance index.

Abstract: Problem: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex. Solution: By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.

Abstract: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex. By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.

Abstract: According to the present invention, phosphorylated peptides and/or phosphorylated proteins are specifically separated. A sample containing a phosphorylated peptide and/or a phosphorylated protein is supplied to a separation unit filled with a metal oxide in the presence of an aliphatic hydroxycarboxylic acid. Upon separation of a phosphorylated peptide and/or a phosphorylated peptide with the use of a separation unit filled with a metal oxide, adsorption of carboxylic acid to an acidic peptide can be prevented in the presence of aliphatic hydroxycarboxylic acid. In addition, aliphatic hydroxycarboxylic acid does not inhibit adsorption of a phosphorylated peptide and a phosphoric acid group in the phosphorylated peptide to a metal oxide.

Abstract: It is an object of the present invention, when determining and identifying an amino acid sequence of a peptide using MS, to obtain additional information from the MS for evaluating validity of an amino acid sequence in a candidate list outputted from an identifying engine. The present invention provides a method of testing an amino acid sequence inferred by searching a peptide-related database based on peptide mass information and/or peptide modification information obtained through mass spectrometry on a peptide, the method comprising the steps: (1) calculating a theoretical value of an isotopic ratio for the peptide from the inferred amino acid sequence and/or the peptide modification information; (2) measuring a measured value of the isotopic ratio for the peptide from the peptide mass information; and (3) comparing the theoretical value and the measured value, and evaluating validity of the inferred amino acid sequence from differences between the theoretical value and the measured value.

Abstract: The present inventor has established protein abundance index (PAI, ?) to determine the protein contents in a protein mixture solution using nanoLC-MSMS data. Digested peptides were analyzed by nanoLC-MS/MS and the obtained results were applied to a Mascot protein identification algorism based on tandem mass spectra. PAI is defined as the number of observed peptides divided by the number of observable peptides per protein. PAI from different concentrations of serum albumin showed linear relationship to the logarithm of the protein concentration. This was also valid for 47 proteins in a mouse whole cell lysate analyzed by single run of nanoLC-MS/MS. On the other hand, Mascot protein scores as well as the number of identified peptides per protein were less correlated to the protein abundance. For absolute quantitation, PAI was converted to exponentially modified PAI (EMPAI, m?), which is proportional to protein contents in the protein mixture.

Abstract: Problem: An object of the present invention is to quantitate with good accuracy, furthermore, quantitate absolutely, one or a plurality of biological molecules in a sample such as a tissue, a biological fluid, a cell, a cell organ or protein complex. Solution: By adding a metabolically isotope labeled biological molecule as an internal standard substance and measuring with a mass spectrometer, quantitating with good accuracy one or a plurality of target molecules in a sample has become possible. In addition, by performing waveform separation processing during mass analysis, a highly accurate quantitative analysis method of mass analysis is provided.

Abstract: A filter filling apparatus serving as a system for manufacturing a tip device according to the present invention comprises, for example, on a base inside a casing, a sheet holder with a sheet-like filter carried thereon and a tip rack where a plurality of tips are held in a matrix-like configuration. Furthermore, a small-diameter filter punching-discharging mechanism having a plurality of small-diameter pipes disposed in a row and movable in the vertical direction and a filter push-in mechanism having a plurality of small-diameter rods disposed in a row and movable in the vertical direction are provided above the sheet holder and the tip rack. A cylinder connected to a nitrogen gas supply unit is connected via piping to the small-diameter pipes.

Abstract: The present invention provides a packing for chromatography. Specifically, it provides a packing for chromatography in which a hydrophilic polymeric material is bound to the surface of a porous carrier, and a carbon chain is chemically bound inside the pore. By the use of the packing according to the present invention for analysis of low molecular weight compounds in a biological sample, a pretreatment such as removal of proteins etc. becomes unnecessary, and the biological sample can be directly analyzed.

Abstract: It is to provide a capillary having adsorbed a polymer. A capillary having absorbed on an inner wall of the capillary, an ionic polymer having positive electric charge and an ionic polymer having negative electric charge alternately, and a process for producing the same.

Abstract: The present invention provides a device capable of simply formulating an arbitrary gradient pattern in a low flow gradient high performance liquid chromatography. That is, the present invention provides a split tubing apparatus for a gradient high performance liquid chromatography, which comprises a solvent inlet part, a split tubing part split into two or more plural tubes, and a solvent outlet part.

Abstract: The present invention provides a packing for chromatography. Specifically, it provides a packing for chromatography in which a hydrophilic polymeric material is bound to the surface of a porous carrier, and a carbon chain is chemically bound inside the pore. By the use of the packing according to the present invention for analysis of low molecular weight compounds in a biological sample, a pretreatment such as removal of proteins etc. becomes unnecessary, and the biological sample can be directly analyzed.

Abstract: A photohardenable resin composition comprising (A) a urethanated acrylic compound represented by formula (I): (A)a—C—(R)4−a wherein R is H or alkyl; a is 3 or 4; and A represents a group of formula (II): wherein D represents —CH2CH2O—, —CH2CH(CH3)O—, —CH2CH2COO— or —(CH2)6COO—; E represents —CH2CH2O—, —CH2CH(CH3)O—, —COCH2CH2O— or —CO(CH2)6O—; G represents a diol or triol residual group; J represents a divalent or trivalent and substituted or unsubstituted hydrocarbon group; L represents —(CH2CH2O)h— (h: integer of 1 to 4), —(CH2CH(CH3)O)j— (j: integer of 1 to 4), or —(CH2CH2O)k—(CH2CH(CH3)O)m— (k, m: integer of 1 to 3 (k+m=2, 3 or 4); b, c, and d are each 0 or 1 (b+c+d=1, 2 or 3); e is 0, 1 or 2; f is 0 or 1 (e+f=1 or 2); and g is 1 or 2, and (B) a radical polymerizable compound at a (A) : (B) r

Abstract: Donepezil hydrochloride, 1-benzyl-4-[(5,6-dimethoxy-1-indanon)-2-yl]methylpiperidine hydrochloride, is provided here in the form of four polymorphs which are stable against heat and humidity in the pharmaceutical use. They can be industrially produced. They are specified by peaks in X-ray powder diffraction pattern and absorption peaks in infrared absorption spectra in potassium bromide.

Abstract: A photocurable resin composition comprising (A) at least one urethane di- or triacrylate, which is a novel compound, (B) a radical polymeriable compound, and (C) a photopolymerization initiator, the weight ratio of the urethane acrylate (A) to the radical polymerizable compound (B) being 65/35 to 25/75; a method of producing a photo-cured object by photo-curing the above composition; a vacuum casting mold obtained by the above method; and a vacuum casting method using the above mold.