Abstract: An object of the present invention it to improve the secretion productivity of a heterologous protein in a methanol-assimilating yeast. The present invention provides a method for producing a heterologous protein, characterized by comprising using a methanol-assimilating yeast with disruption of the VPS gene as a host for secretory production of the heterologous protein.

Abstract: An object of the present invention it to improve the secretion productivity of a heterologous protein in a methanol-assimilating yeast. The present invention provides a method for producing a heterologous protein, characterized by comprising using a methanol-assimilating yeast with disruption of the VPS gene as a host for secretory production of the heterologous protein.

Abstract: According to this invention, by introducing genes encoding hexulose-6-phosphate synthase and 6-phosphohexulose isomerase into a plant to have the genes expressed in the chloroplast of the plant, a transgenic plant having a pathway to assimilate formaldehyde through the Calvin cycle is provided. The transgenic plant according to this invention has resistance to formaldehyde and is capable of reducing the level of environmental formaldehyde significantly. Therefore, it is assumed that the transgenic plant according to this invention can be used to purify environmental condition, by placing it in a residence or in an office.

Abstract: This invention relates to a promoter for dihydroxyacetone synthase gene from Candida boidinii; an expression cassette or vector comprising the promoter and a heterologous gene; a transformant transformed with this vector; and a method for producing an expression product of the heterologous gene which comprises culturing the transformant to express the heterologous gene.

Abstract: The present invention discloses a method for culturing microorganisms having a methanol metabolic pathway in which an expression unit is introduced that comprises a target gene linked downstream from a promoter that can be induced by methanol; wherein, during the culturing period, and including the period during which methanol is continuously or periodically added, the rate of addition is adjusted to a rate equal to or less than the maximum methanol consumption rate of said microorganisms.

Abstract: A method for producing phytase using a Candida boidinii transformant, by which phytase is secreted to liquid medium without substantially increasing the number of the transformant cells is disclosed. In this method, cells of a Candida boidinii transformant which is transformed with a recombinant vector comprising a promoter inducible with methanol and a gene located downstream of said promoter and operably linked to said promoter, which gene encodes a polypeptide having phytase activity, are cultured in a liquid culture medium containing phosphoric acid or a phosphate compound in an amount effective of restricting proliferation of said cells of Candida boidinii transformant such that the amount of said cells of Candida boidinii transformant is not substantially increased, while adding methanol to the culture medium, so as to make said cells secrete said phytase.

Abstract: The present invention relates to a fructosyl amino acid oxidase that is obtainable from a culture of a strain of the genus Aspergillus. This strain grows in a selective medium containing at least either of fructosyl lysine and fructosyl-N.sup..alpha. -Z-lysine and is capable of producing a fructosyl amino acid oxidase.

Abstract: A promoter for a formate dehydrogenase gene from Candida boidinii, substantially comprising a 190 bp or more continuous nucleotide sequence selected from the nucleotide sequence of SEQ ID NO:1; a promoter for a formate dehydrogenase gene from Candida boidinii, substantially comprising the nucleotide sequence of SEQ ID NO:1, 48, 49 or 50; a terminator for a formate dehydrogenase gene from Candida boidinii, substantially comprising the nucleotide sequence of SEQ ID NO:2; a gene expression cassette comprising said promoter, a heterologous gene and said terminator; a recombinant expression vector comprising said gene expression cassette; a transformant transformed with said recombinant expression vector; a process for producing an expression product of a heterologous gene, which comprises culturing said transformant and recovering an expression product of a heterologous gene from the culture.

Abstract: A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.

Abstract: A protein derived from a strain of methylotrophic yeast which has a protein disulfide isomerase activity having the amino acid sequence as set forth in SEQ ID No. 1, or protein in which the amino acid sequence has been modified by deletion or addition of one or a few amino acids, or substitution with other amino acid(s) and which has a protein disulfide isomerase activity; and a process for production thereof.

Abstract: The present invention provides a recombinant protein which shows fructosyl amino acid oxidase activity, a DNA encoding the same, an expression vector containing the DNA, a transformant transformed by the expression vector, and the method of preparing recombinant fructosyl amino acid oxidase by culturing the resultant transformant, and the recombinant fructosyl amino acid oxidase thus obtained.

Abstract: A novel fructosyl amino acid oxidase derived from genus Penicillium, which is active on both of fructosyl lysine and fructosyl valine, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme is provided.

Abstract: A novel fructosyl amino acid oxidase derived from genus Fusarium or Gibberella, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme, a process for producing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine as a substrate of a fructosyl amino acid oxidase, which is useful for screening and/or culturing a microorganism capable of producing the enzyme, and a medium containing fructosyl lysine and/or fructosyl N.sup..alpha. -Z-lysine for screening and/or culturing a microorganism capable of producing a fructosyl amino acid oxidase is provided.

Abstract: The present invention relates to an expression vector which is induced by methanol and/or glycerol utilizing an alcohol oxidase gene of a methylotrophic yeast; a recombinant methylotrophic yeast containing said vector and capable of accumulating a significantly high amount of an expression product of a heterologous gene; and a method for producing useful products utilizing said recombinant.The expression vector is constructed by utilizing the promoter and terminator of an alcohol oxidase gene of methylotrophic yeast. Further, a significantly high amount of adenylate kinase, cytochrome C552 or peroxidase is produced by using said expression vector.

Abstract: A novel fructosyl amino acid oxidase derived from genus Fusarium, which is active on both of fructosyl lysine and fructosyl valine, a process for producing the enzyme, an assay of an amadori compound using the enzyme, a reagent or a kit containing the enzyme is provided.