Abstract: A nucleic acid fragment including a polymerase binding sequence and a transposase binding sequence, in which the polymerase binding sequence or the transposase binding sequence contains a caged nucleotide residue; a specific binding substance; a method for binding a polymerase or a transposase to the nucleic acid fragment; a method for inserting the nucleic acid fragment in a vicinity of a binding region of a DNA-binding protein bound to a DNA molecule; and a method for gene-amplifying the DNA molecule into which the nucleic acid fragment is inserted are provided.

Abstract: A biomolecule structure detection probe, wherein a specific binding substance having a specific binding activity to a biomolecule structure is linked to a labeling substance via a linker including a disulfide bond. In addition, a biomolecule structure detection kit including the biomolecule detection probe and a reagent for cleaving the disulfide bond. In addition, a biomolecule structure detection kit including a linker for linking a specific binding substance having a specific binding activity to a biomolecule structure with a labeling substance, the linker including a disulfide bond; and a labeling substance capable of being bonded to the linker; and a reagent for cleaving the disulfide bond. In addition, a method for detecting a biomolecule structure using the biomolecule structure detection probe.

Abstract: A method is provided for integrating a DNA fragment of a desired base sequence into a site located adjacent to a binding region of a DNA-binding protein bound to a DNA molecule, the method including bringing the DNA fragment having a base sequence including a transposase-binding sequence and the desired base sequence close to the binding region using a specific binding substance to the DNA-binding protein, binding transposase to the transposase-binding sequence, and activating the transposase such that the DNA fragment of the desired base sequence is integrated into the site located adjacent to the binding region.

Abstract: A method is provided for integrating a DNA fragment of a desired base sequence into a site located adjacent to a binding region of a DNA-binding protein bound to a DNA molecule, the method including bringing the DNA fragment having a base sequence including a transposase-binding sequence and the desired base sequence close to the binding region using a specific binding substance to the DNA-binding protein, binding transposase to the transposase-binding sequence, and activating the transposase such that the DNA fragment of the desired base sequence is integrated into the site located adjacent to the binding region.

Abstract: A method for determining a nucleic acid sequence of a target gene expressed in a subject cell, the method including: comprehensively determining mRNA nucleic acid sequences in the subject cell, and identifying a nucleic acid sequence having a nucleic acid sequence of a portion of the target gene, from among the determined mRNA nucleic acid sequences, in which the identified nucleic acid sequence is a nucleic acid sequence of the target gene.