Patents by Inventor Yayoi KAMEDA

Yayoi KAMEDA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element
    Patent number: 11781146
    Abstract: The present invention provides mammalian cell expression vectors that impart to mammalian host cells an ability to produce high levels of foreign gene-derived proteins. A ubiquitously acting chromatin opening element (UCOE) is introduced into an expression vector that has a plasmid DNA integrated into a transcriptional hot spot on the chromosome of a dihydrofolate reductase gene-deficient host cell so that it allows for selection of strains that grow in hypoxanthine-thymidine (hereinafter denoted as HT)-free medium, whereby transformants will produce a protein of interest in increased amounts.
    Type: Grant
    Filed: May 13, 2019
    Date of Patent: October 10, 2023
    Assignees: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY, FUSO PHARMACEUTICAL INDUSTRIES, LTD.
    Inventors: Yasuhiko Suzuki, Miki Nakagawa, Yayoi Kameda, Satoru Konnai, Tomohiro Okagawa, Naoya Maekawa, Shinya Goto, Yamato Sajiki, Kazuhiko Ohashi, Shiro Murata, Yuzuru Kitahara, Keiichi Yamamoto
  • NOVEL VECTOR AND USE THEREOF
    Publication number: 20210254079
    Abstract: The present invention provides mammalian cell expression vectors that impart to mammalian host cells an ability to produce high levels of foreign gene-derived proteins. A ubiquitously acting chromatin opening element (UCOE) is introduced into an expression vector that has a plasmid DNA integrated into a transcriptional hot spot on the chromosome of a dihydrofolate reductase gene-deficient host cell so that it allows for selection of strains that grow in hypoxanthine-thymidine (hereinafter denoted as HT)-free medium, whereby transformants will produce a protein of interest in increased amounts.
    Type: Application
    Filed: May 12, 2019
    Publication date: August 19, 2021
    Applicants: National University Corporation Hokkaido University, Fuso Pharmaceutical Industries, Ltd.
    Inventors: Yasuhiko SUZUKI, Miki NAKAGAWA, Yayoi KAMEDA, Satoru KONNAI, Tomohiro OKAGAWA, Naoya MAEKAWA, Shinya GOTO, Yamato SAJIKI, Kazuhiko OHASHI, Shiro MURATA, Yuzuru KITAHARA, Keiichi YAMAMOTO