Abstract: A press-based fluidic transportation and diagnosis device includes a top plate, a first reagent storage chamber, a second reagent storage chamber, a sample inflow chamber, a detection chamber, a through hole and a gas outlet hole. On end of the second reagent storage chamber is communicated with the first reagent storage chamber by a first flow path. The sample inflow chamber is communicated with the other end of the second reagent storage chamber by a second flow path. On end of the detection chamber is communicated with the sample inflow chamber by a third flow path. The through hole is used for accommodating a press-based driving unit, and is communicated with the other end of the detection chamber by a fourth flow path. The gas outlet hole is communicated with the through hole by a fifth flow path.