Abstract: A method for detecting the zeta potentials of nanopores and nanoparticles mainly uses an electrokinetic mechanism with a force balance exerted on particles in a nanopore and a current sensing technology to measure the zeta potential of the nanopore accurately, and then uses the measured zeta potential of the pore to further measure the zeta potential of the electrically charged nanoparticle passing through the pore. This method does not need to analyze the detailed spectrum of the current blockage signals and purchase expensive standard item particles, so that this method has high accuracy and less limitation than the conventional method and achieves the effects of simplifying the measurement process and lowering the measurement cost significantly. For soft nanoparticles, this method can sense the zeta potential of particles more accurately to improve the value of the method of this invention.