Patents by Inventor Yevgeniy Belousov

Yevgeniy Belousov has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Droplet digital PCR with short minor groove probes
    Patent number: 9328384
    Abstract: Methods for the detection of ddPCR assay-generated amplicons by short minor groove binder-fluororophore-oligonucleotide-quencher (MGB-Fl-oligo-Q) probes. The short MGB-Fl-oligo-Q probes not only reduce background, but also show improved mismatch discrimination when compared to the same length non-MGB probes for detecting the ddPCR generated targets at room temperature.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: May 3, 2016
    Assignee: ELITECHGROUP B.V.
    Inventor: Yevgeniy Belousov
  • DROPLET DIGITAL PCR WITH SHORT MINOR GROOVE PROBES
    Publication number: 20140335515
    Abstract: Methods for the detection of ddPCR assay-generated amplicons by short minor groove binder-fluororophore-oligonucleotide-quencher (MGB-Fl-oligo-Q) probes. The short MGB-Fl-oligo-Q probes not only reduce background, but also show improved mismatch discrimination when compared to the same length non-MGB probes for detecting the ddPCR generated targets at room temperature.
    Type: Application
    Filed: May 2, 2014
    Publication date: November 13, 2014
    Applicant: Elitech Holding B.V.
    Inventor: Yevgeniy Belousov
  • HYPERTHERMOSTABLE ENDONUCLEASE IV SUBSTRATE PROBE
    Publication number: 20130022976
    Abstract: The present invention relates to a hyperthermostable endonuclease IV substrate probe to be used in nucleic acid assay methods which can be carried out using hyperthermostable enzymes, including detection of target nucleic acids, and detection of nucleic acid polymorphism.
    Type: Application
    Filed: September 12, 2012
    Publication date: January 24, 2013
    Inventors: Yevgeniy Belousov, Eugene Lukhtanov
  • HYPERTHEROMOSTABLE ENDONUCLEASE IV SUBSTRATE PROBE
    Publication number: 20110151457
    Abstract: The present invention relates to a hyperthermostable endonuclease IV substrate probe to be used in nucleic acid assay methods which can be carried out using hyperthermostable enzymes, including detection of target nucleic acids, and detection of nucleic acid polymorphism.
    Type: Application
    Filed: December 16, 2010
    Publication date: June 23, 2011
    Inventors: Yevgeniy Belousov, Eugeny Lukhtanov
  • Single nucleotide polymorphism analysis of highly polymorphic target sequences
    Patent number: 7718374
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: May 18, 2010
    Assignee: Elitech Holding B.V.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • METHOD FOR GENERATING TARGET NUCLEIC ACID SEQUENCES
    Publication number: 20090092967
    Abstract: The present invention provides methods of generating target nucleic acids for amplification using nicking enzymes and methods for amplifying the generated target nucleic acids.
    Type: Application
    Filed: June 26, 2007
    Publication date: April 9, 2009
    Applicant: Epoch Biosciences, Inc.
    Inventors: Zuxu Yao, Graham Lidgard, Yevgeniy Belousov
  • SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS OF HIGHLY POLYMORPHIC TARGET SEQUENCES
    Publication number: 20090087922
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Application
    Filed: January 28, 2008
    Publication date: April 2, 2009
    Applicant: Epoch Biosciences, Inc.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • Single nucleotide polymorphism analysis of highly polymorphic target sequences
    Patent number: 7348146
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Grant
    Filed: September 29, 2004
    Date of Patent: March 25, 2008
    Assignee: Epoch Biosciences, Inc.
    Inventors: Yevgeniy Belousov, Robert O. Dempcy, Sergey G. Lokhov, Alexei Vorobiev
  • Process for the synthesis of pyrazolopyrimidines
    Publication number: 20070060744
    Abstract: The present invention provides a nucleoside comprising a pyrazolopyrimidine base and a process for producing the same. In particular, the processes of the present invention comprises using a halogenated pyrazolopyrimidine base and removing the halogen after the base is coupled to a sugar moiety. The presence of the halogen on the nucleoside base allows facile and economical production of a large quantity of nucleosides.
    Type: Application
    Filed: June 6, 2006
    Publication date: March 15, 2007
    Applicant: Epoch Biosciences, Inc.
    Inventors: Robert Dempcy, A. Adams, Michael Reed, Yevgeniy Belousov
  • Real-time linear detection probes: sensitive 5'-minor groove binder-containing probes for PCR analysis
    Publication number: 20070003957
    Abstract: Oligonucleotide probes/conjugates are provided along with method for their use in assays to monitor amplification wherein the signal produced does not rely on 5? nuclease digestion.
    Type: Application
    Filed: June 19, 2006
    Publication date: January 4, 2007
    Applicant: Epoch Biosciences, Inc.
    Inventors: Irina Afonina, Yevgeniy Belousov, Robert Dempcy, Igor Kutyavin, Sergey Lokhov, Eugeny Lukhtanov
  • Process for the synthesis of pyrazolopyrimidines
    Publication number: 20060014943
    Abstract: The present invention provides a nucleoside comprising a pyrazolopyrimidine base and a process for producing the same. In particular, the processes of the present invention comprises using a halogenated pyrazolopyrimidine base and removing the halogen after the base is coupled to a sugar moiety. The presence of the halogen on the nucleoside base allows facile and economical production of a large quantity of nucleosides.
    Type: Application
    Filed: September 19, 2005
    Publication date: January 19, 2006
    Applicant: Epoch Biosciences, Inc.
    Inventors: Robert Dempcy, A. Adams, Michael Reed, Yevgeniy Belousov
  • Single nucleotide polymorphism analysis of highly polymorphic target sequences
    Publication number: 20050118623
    Abstract: Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked.
    Type: Application
    Filed: September 29, 2004
    Publication date: June 2, 2005
    Applicant: Epoch Biosciences, Inc.
    Inventors: Yevgeniy Belousov, Robert Dempcy, Sergey Lokhov, Alexei Vorobiev