Abstract: A method for manufacturing a measuring kit is disclosed. The method for manufacturing a measuring kit includes steps of providing a carrier including a substrate having a surface; coating a silane onto the surface of the substrate; providing an organic molecule; adding the organic molecule onto the carrier; outputting a microwave energy by a coaxial cable; and emitting the microwave energy through a radiator to microwave the carrier and the organic molecule uniformly to coat the organic molecule onto the carrier carrying the silane, wherein the measuring kit is used to apply an enzyme-linked immunosorbent assay.

Abstract: A sensing method is disclosed. The sensing method includes steps of providing a carrier including a hole having a bottom, wherein a plurality of spaced apart first nanoparticles are disposed on the bottom; coating a sensing molecule in the hole; providing a testing solution having a testing parameter to the hole, wherein the testing solution has a complex including a testing molecule and a second nanoparticle, and a specific binding occurs between the testing molecule and the sensing molecule; centrifuging the carrier to subside the complex; washing the hole; and measuring a synthetic spectral signal change of the first nanoparticle and the second nanoparticle according to a degree of the specific binding between the testing molecule and the sensing molecule to determine the testing parameter of the testing solution.

Abstract: A method for manufacturing a measuring kit is disclosed. The method for manufacturing a measuring kit includes steps of providing a carrier including a substrate having a surface; coating a silane onto the surface of the substrate; providing an organic molecule; adding the organic molecule onto the carrier; outputting a microwave energy by a coaxial cable; and emitting the microwave energy through a radiator to microwave the carrier and the organic molecule uniformly to coat the organic molecule onto the carrier carrying the silane, wherein the measuring kit is used to apply an enzyme-linked immunosorbent assay.

Abstract: A sensing chip for sensing a first organic molecule in a sample is disclosed. The sensing chip includes a substrate, wherein there are a plurality of separated nanometers on the substrate and a plurality of local substrate surfaces among the plurality of separated nanometers; a silane fixed on the plurality of local substrate surfaces; and a second organic molecule fixed on surfaces of the separated nanometers, wherein there is specificity between the second organic molecule and the first organic molecule.

Abstract: A sensing method, comprising steps of causing a first molecule to be adjacent to one of a plurality of first nanoparticles spacedly disposed on a detachable chip; adding a target object to contact the first molecule; and measuring a spectral signal, wherein a variation of the spectral signal of the plurality of first nanoparticles occurs when the target object demonstrates a first specific binding with the first molecule.

Abstract: A detecting method is herein disclosed. The method includes steps of providing an eukaryotic cell, having a cell nucleus and a cell membrane, wherein the cell nucleus endogenetically translates a first receptor and a second receptor, and wherein the first receptor and the second receptor pass through the cell nucleus and translocate to the cell membrane; coupling the first receptor to a first bioactive ligand with a quantum dot; washing the cell coupled with the first bioactive ligand and the quantum dot by centrifugation; coupling the second receptor to a second bioactive ligand with a magnetic bead; washing the cell coupled with the first bioactive ligand and the second bioactive ligand by magnetic separation; irradiating a exciting energy for the quantum dot to emit a fluorescence, wherein the quantum dot coupled with the cell is excited in a pH range from pH 9 to pH 14; and detecting the fluorescence.

Abstract: A cell detecting system is herein disclosed, wherein a complex is formed with a first bioactive ligand coupling to a quantum dot and recognizing and coupling to a first receptor of a cell and a second bioactive ligand coupling to a magnetic bead and recognizing and coupling to a second receptor of the cell. The magnet is configured for attracting the complex. A quantum dot measuring system configured for measuring the fluorescence of the complex includes an excitation light source, an optical system, a detecting sensor and a data capturing unit, wherein the detecting sensor includes a photomultiplier tube measuring florescence of the quantum dot excited by the excitation light source. The present invention achieves the goal of specific cell detection with high sensitivity without performing cell incubation. A quantum dot measuring system is also herein disclosed.