Abstract: Provided is a method for diagnosing and monitoring progression of cancer or effectiveness of a therapeutic treatment. The method includes detecting a methylation level of at least one gene in a biological sample containing circulating free DNA. Also provided are primer pairs and probes for diagnosis or prognosis of cancer in a subject in need thereof.

Abstract: A method and system for processing online/offline multimedia data are disclosed. The system comprises a remote mainframe, a local host and a portable apparatus. When the portable apparatus is electrically connected with the remote mainframe and the local host, the portable apparatus can immediately exchange multimedia data exchange with the remote mainframe and the local host. When the portable apparatus is not electrically connected with the remote mainframe and the local host, the portable apparatus can independently process the multimedia data previously received from the remote mainframe and the local host; also the portable apparatus can update the multimedia data with the remote mainframe and the local host when the portable apparatus is electrically reconnected with the remote mainframe and the local host. With the system configuration, the present invention features online/offline multimedia-data processing capabilities.