Abstract: Disclosed are the methods for the detection and/or quantification of an analyte in liquid sample. The same principle can be applied in the case of detection and/or quantification of more than one analyte present in the sample. Devices of the invention include a sample application zone, a mobilization zone containing a mobile or mobilizable first binder that is specific for the analyte, a absorption zone containing immobilized analyte or analyte analog, a mobilization zone containing a mobile or mobilizable labeled second binder capable of binding to the first binder, and a capture zone or test zone containing an immobilized third binder capable of binding to the complex of the analyte, the first binder and the second binder. The color development in the capture zone or the test zone is indicative of presence or the concentration of the analyte in the test sample.