Abstract: Highly efficient methods are provided for preparing key intermediates in the synthesis of Compound (I), which are broadly applicable and can provide selected components having a variety of substituents groups.

Abstract: A method of determining the likelihood that a subject with cancer will respond to an immunotherapy comprising a checkpoint inhibitor. The method comprises detecting the level of histamine in a biological sample obtained from the subject. Subjects determined to have a low likelihood of responding to the immunotherapy can be administered an alternative therapy or administered an antihistamine in conjunction with the immunotherapy. Methods of treating cancer patients are also provided, as are kits and systems for histamine detection, recordation, and responder status determination.

Abstract: Provided are an enzyme immobilization carrier and a preparation method therefor, and an immobilized enzyme and a preparation method therefor. The enzyme immobilization carrier includes a resin ball matrix, an —N(CH2OOH)2 group linked on the resin ball matrix by means of a chemical bond, and a metal ion adsorbed on the —N(CH2OOH)2 group in a chelating manner through coordination. The resin ball matrix is an amino-type methacrylic resin and/or an epoxy-type methacrylic resin, and the resin ball matrix has a particle size of 200-700 ?m. Therefore, the problems in the related art that an enzyme immobilization carrier is relatively poor in enzyme compatibility and poor in immobilization effect, and an immobilized enzyme is relatively poor in activity, and relatively poor in stability during repeated use are solved.

Abstract: A calculation method for fractal dimensions of shale pores includes steps as follows. S1: multiple shale samples from a target stratum of a study area are obtained, parameter values of geological parameters of each of the multiple shale samples are obtained, followed by dividing the multiple shale samples into target shale samples and experimental shale samples. S2: PCA is performed on the parameter values to obtain principal components representing a variation of the geological parameters. fractal dimensions are calculated based on an existing fractal dimension calculation method. S3: the principal components are used as independent variables and the fractal dimensions are used as dependent variables, followed by performing regression analysis to obtain a quantitative calculation model. S4: the fractal dimensions of the target shale sample are calculated according to the parameter values and the quantitative calculation model for the fractal dimension based on the parameter values.

Abstract: Aspects of the present disclosure include system, methods, and software for buffer insertions. In one example, a method includes receiving a clock signal network layout, the clock signal network layout comprising a clock source electrically coupled to a plurality of clock sinks via a plurality of net segments, a first hierarchy that bounds a first region of the clock signal network layout, and a second hierarchy that bounds a second region of the clock signal network layout. The method additionally includes creating a graph representative of the clock signal network layout, the graph comprising a plurality of logical edges, and identifying a to-be-inserted buffer location comprising a location on a net segment of the plurality of net segments to insert a buffer. The method further includes selecting a selected logical edge to insert the buffer based on the to-be-inserted buffer location, the first hierarchy, the second hierarchy, or a combination thereof.

Abstract: Provided are a catalyst loaded with a TEMPO-type compound, and a preparation method and use thereof. The catalyst loaded with the TEMPO-type compound uses a polymer resin as a carrier, and the polymer resin is a polystyrene resin containing a chloromethyl or bromomethyl, herein the content of the chloromethyl or bromomethyl in the polymer resin is 0.5-5.0 mmol g?1.

Abstract: This invention introduces a kinetic energy recovery wind-wave integrated system for offshore wind power generation. The system consists of a semi-submersible platform equipped with a fan and an internal wave energy device. The device includes a shell housing a Power Take-Off (PTO) system, featuring a permanent magnet synchronous linear motor and an active controller. The motor's stator is fixed inside the shell, while its mover is connected to a counterweight block outside the stator, linked to the shell's top via a spring. Limiters are installed at both ends of the shell to restrict the counterweight block's movement. This system utilizes the wave energy device to absorb kinetic energy, which otherwise affects wind turbine stability, and converts it into usable electrical energy via the PTO system. This enhances the stability and safety of offshore wind turbine power generation.

Abstract: The present disclosure provides compositions and methods related to opioid detection. In particular, the present disclosure provides aptamer-based colorimetric assays for the visualization and identification of specific opioids (natural, semi-synthetic, synthetic and the derivatives and analogs thereof) in a manner that is rapid, specific, and sensitive.

Abstract: Provided are an esterase mutant and the use thereof. The esterase mutant obtained by means of rational design and several rounds of evolution screening with enzymes on the basis of an amino acid sequence as shown in SEQ ID NO: 1 is changed in terms of protein structure and function compared with a wild-type esterase; in practical use, the catalytic activity and/or stereoselectivity of the esterase mutant is greatly improved; and when a system contains some organic cosolvents, the esterase mutant still has relatively stable catalytic activity and/or stereoselectivity. In addition, the improvement of the catalytic activity and/or stereoselectivity of the esterase mutant reduces the use amount of the enzyme to a certain extent and reduces the difficulty of post-treatment, and therefore the esterase mutant is suitable for industrial production.

Abstract: Provided are an immobilized enzyme and an application thereof in continuous production. The immobilized enzyme is a Polyethyleneimine (PEI)-modified immobilized enzyme, and includes: an enzyme, which includes Amine Dehydrogenase (AmDH) and/or Formate Dehydrogenase (FDH); and a carrier, which is a cyanuric chloride-activated amino carrier. The problem in the prior art of poor performance of an immobilized enzyme is solved, the catalytic activity and reusability of the immobilized enzyme are improved, and the immobilized enzyme is suitable for the field of enzyme immobilization.

Abstract: A method for continuously synthesizing N-Boc-2,5-dihydropyrrole is provided. The method includes the following steps: cis-1,4 dichloro-2 butene is dissolved in an organic solvent, to prepare solution A; urotropine is dissolved in an organic solvent, to prepare solution B; the solution A and solution B are fed into a first continuous reaction device; a product overflow system is transferred to a second continuous reaction device, and concentrated hydrochloric acid is fed into the same; a product overflow system of the second continuous reaction device is transferred to a third continuous reaction device, and potassium carbonate is fed into the same; a product overflow system of the third continuous reaction device is transferred to a fourth continuous reaction device, and Boc acid anhydride solution is fed into the same; and a product of the fourth continuous reaction device is subjected to continuous solid-liquid separation, concentration and distillation, to obtain N-Boc-2,5-dihydropyrrole.

Abstract: The present invention is directed to a compound represented by Structural Formula (A): or a pharmaceutically acceptable salt thereof. The variables for Structural Formula (A) are defined herein. Also described is a pharmaceutical composition comprising the compound of Structural Formula (A) and its therapeutic use.

Abstract: Disclosed are a passive triggered-power electronic tap-changer device and a contact device. The power electronic tap-changer device comprises: two taps, a main contact, two auxiliary contacts, two trigger contacts, an output terminal, two thyristors, and four voltage divider resistors, which can achieve the passive triggering of a power electronic switch by means of controlling the auxiliary contacts and the trigger contacts. Provided in the present invention is a contact device for the passive triggered-power electronic tap-changer, comprising: at least two stationary contacts, a main contact, two thyristor trigger contacts, two thyristor auxiliary contacts, and a drive shaft, wherein the main contact, the thyristor trigger contacts, and the thyristor auxiliary contacts are fixed to the drive shaft, the contact device can switch the power electronic switch to different stationary contacts by means of controlling the rotation of the drive shaft, and maintains the switching time sequence of passive triggering.

Abstract: Methods of activating immune response for treating cancer, infections, or other immune-deficient diseases in patients are provided. Also provided are compositions, formulations, and kits for use in such methods, which comprise dendritic cells (DCs), or extracellular vesicles derived therefrom (DC-EVs), in which src homologous region 2 domain-containing phosphatase-1 (SHIP1) expression and/or activity is inhibited and which have been loaded with disease-specific or disease-associated antigens or peptides. Such SHIP1-modified DC-EVs or DCs, or formulations thereof, may be administered to a patient as monotherapy, and/or as a component of combinational therapies with other therapies in the prevention and treatment of cancer and for boosting antigen-specific immune function in the treatment of other diseases. Also provided are methods of making such SHIP1-modified DC-EVs or DCs and formulations thereof.

Abstract: Provided is a ketoreductase mutant and a method for producing chiral alcohol using the same. The ketoreductase mutant has a sequence with amino acid mutations in the sequence shown in SEQ ID NO:1. The mutation sites include at least one of the following positions: 6th position, 21st position, 42nd position, 58th position, 61st position, 76th position, 87th position, 94th position, 96th position, 108th position, 113th position, 117th position, 144th position, 146th position, 147th position, 149th position, 151st position, 152nd, 156th position, 165th position, 177th position, and 198th position.

Abstract: Provided are a transaminase mutant and an application thereof. The transaminase mutant has an amino acid mutation based on a sequence shown in SEQ ID NO: 1, the amino acid mutation being a single position mutation from among W60Y, Y168A, V379W, V379L, V379M, C418Q and C418W or a combination thereof. The activity, stability, and tolerance to temperature, pH and organic solvents of such transaminase mutants are improved. The present application solves the problem of poor tolerance of extreme environments by transaminase in the prior art, and is suitable for the field of enzyme engineering.

Abstract: A dispensing system includes a liquid storage box having a liquid storage cavity and a liquid outlet in communication with the liquid storage cavity, and a liquid mixing box having a liquid mixing cavity and a liquid inlet and a mixed liquid outlet that are in communication with the liquid mixing cavity. The liquid mixing box includes a visible region through which the liquid mixing cavity is observable. The dispensing system further includes a dispensing device connected to the liquid outlet and the liquid inlet. The dispensing device is configured to deliver a detergent in the liquid storage cavity to the liquid mixing cavity.

Abstract: The present disclosure relates to a fastening elastic card bag, a fastening method and application. A card bag body comprises a left shell and a right shell. The left shell and the right shell are detachably connected through an inner shell and a shell. The left shell and the right shell are connected through a shell connector. After the left shell and the right shell are connected through the shell connector, an opening is formed, and articles in an accommodating cavity are put into or taken out of the card bag through the opening. The shell connector is an elastic connector.

Abstract: Disclosed are a modified epoxy resin immobilized enzyme, a preparation method therefor and an application thereof. Herein, the preparation method includes the following steps: modifying an epoxy resin, adding a polyethyleneimine to a modified epoxy resin for further modification, and then adding an enzyme to be immobilized and a glutaraldehyde for immobilization, to obtain the modified epoxy resin immobilized enzyme. The epoxy resin is modified, the polyethyleneimine is added to the modified epoxy resin for the further modification, and an aldehyde group in the resin and an amino group in the polyethyleneimine are covalently bound to each enzyme, then it is activated by the bifunctional reagent glutaraldehyde.