Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: The disclosure discloses a magnetic bias active suppression method and system of a hybrid distribution transformer, whether the HDT is closed or not is judged based on the grid-side current, and accordingly smooth switching of the two sets of control strategies is achieved; excitation currents of an HDT main transformer and an isolation transformer are calculated based on a magnetic potential balance principle, and nonlinear feedback is performed, and the excitation currents are superposed after an original control instruction of an HDT converter, so that magnetic bias suppression is realized on the basis of ensuring an HDT grid-side current and load voltage control function. According to the system, a complex and expensive iron core magnetic flux sensor does not need to be additionally arranged, and the DC magnetic bias of the HDT under any working condition can be eliminated while the basic control function of the HDT is not affected.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: Among other things, the present disclosure provides compositions and methods for analysis of cytosine modifications in nucleic acid sequences. In some embodiments, the present disclosure provides subtraction-free methods for detection of cytosine modifications in one or more nucleic acid sequences.

Abstract: A method for suppressing magnetizing inrush current of the transformer with flux linkage control includes connecting a small-capacity direct current/alternating current (DC/AC) converter with the secondary winding or auxiliary winding of transformer, detecting the primary side phase voltage before closing load, inducing the core flux linkage reference according to the relationship between the winding voltage and core flux linkage. The core flux linkage closed-loop PI control system is constructed to control the converter voltage in the synchronous coordinate, then the core flux linkage can track its reference with no static error, thus the sinusoidal flux linkage with 90-degree difference from the grid voltage can be pre-established in the core before no-load closing. By these methods, no matter when the main transformer closes, the core flux linkage is always in the steady state, and the magnetizing inrush current can be eliminated completely.

Abstract: The present disclosure provides a bisulfite-free, long-read, base-resolution method named long-read TAPS (lrTAPS) for detecting 5-Methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in a nucleic acid sequence. lrTAPS comprises mild enzymatic and chemical reactions to detect 5mC and 5hmC, the two major epigenetic marks found in the mammalian genome, quantitatively at base-resolution without affecting unmodified cytosine.

Abstract: A magnetic integrated hybrid distribution transformer includes a main transformer, a series isolation transformer and a converter, wherein: an iron core includes an iron beam unit, an iron yoke unit and a leakage magnetic core unit. The main transformer includes secondary windings, primary windings and control windings all of which are layer-windings and wound around main transformer iron beams. The series isolation transformer includes converter side windings and grid side windings all of which are pancake-windings and wound around isolation transformer iron beams. The converter side windings and the control windings are respectively connected with the converter by the star connection with neutral point. Leakage magnetic cores are respectively inserted between the primary windings and the control windings or between the converter side windings and the grid side windings, so as to achieve magnetic integration design of the transformer and output connection inductor of the converter.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: This disclosure provides methods for cost-effective bisulfite-free identification in DNA, including whole genomic DNA, of the locations of one or more of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine. The methods described herein are based on the conversion of modified cytosine (5mC, 5hmC, 5fC, 5caC) to dihydrouracil (DHU), for example by TET-assisted pyridine borane treatment, followed by endonuclease cleavage of the DHU, and identification of the cleavage site, which corresponds to the location of the modified cytosine.

Abstract: A method for suppressing magnetizing inrush current of the transformer with flux linkage control includes connecting a small-capacity direct current/alternating current (DC/AC) converter with the secondary winding or auxiliary winding of transformer, detecting the primary side phase voltage before closing load, inducing the core flux linkage reference according to the relationship between the winding voltage and core flux linkage. The core flux linkage closed-loop PI control system is constructed to control the converter voltage in the synchronous coordinate, then the core flux linkage can track its reference with no static error, thus the sinusoidal flux linkage with 90-degree difference from the grid voltage can be pre-established in the core before no-load closing. By these methods, no matter when the main transformer closes, the core flux linkage is always in the steady state, and the magnetizing inrush current can be eliminated completely.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: An attenuated strain of cucumber green mottle mosaic virus (CGMMV) is useful to protect cucumber plants from infection with the wild-type infectious CGMMV strain. The genome of the attenuated virus contains at least one mutation or group of mutations selected from c.4969G>A, c.3334C>T, and a group of at least six of the mutations c.315G>A; c.1498A>G; c.1660C>T; c.3430C>T; c.3528A>G; c.4144C>T; c.4248C>T; and c.6228C>T. These mutated genomes encode one or more mutations selected from R163711 in the 186 kDa readthrough replication protein, A1092V in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and at least six mutations selected from G86S, E480G, S534F, A1124V, N1157D, P1362L, P1397S in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and the A156V mutation in the coat protein.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: A method for preparing titanium-containing molecular sieves include the following steps: irradiating a mixed solution containing a silicon source, a template and a titanium source by a light source containing ultraviolet light before crystallization, and then subjecting the mixed solution to crystallization and post-treatment to obtain the titanium-containing molecular sieve. In the method for preparing titanium-containing molecular sieve of the present invention, the mixed solution containing the silicon source, the template and the titanium source is treated with the ultraviolet light, and free radicals generated by the ultraviolet light can effectively dissociate titanium oligomers and accelerate the hydrolysis of the silicon source, and thus match the hydrolysis rate of a silicon-titanium precursor, thereby preventing a titanium monomer from self-polymerization to form non-framework titanium.

Abstract: An attenuated strain of cucumber green mottle mosaic virus (CGMMV) is useful to protect cucumber plants from infection with the wild-type infectious CGMMV strain. The genome of the attenuated virus contains at least one mutation or group of mutations selected from c.4969G>A, c.3334C>T, and a group of at least six of the mutations c.315G>A; c.1498A>G; c.1660C>T; c.3430C>T; c.3528A>G; c.4144C>T; c.4248C>T; and c.6228C>T. These mutated genomes encode one or more mutations selected from R163711 in the 186 kDa readthrough replication protein, A1092V in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and at least six mutations selected from G86S, E480G, S534F, A1124V, N1157D, P1362L, P1397S in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and the A156V mutation in the coat protein.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methyl-cytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: An attenuated strain of cucumber green mottle mosaic virus (CGMMV) is useful to protect cucumber plants from infection with the wild-type infectious CGMMV strain. The genome of the attenuated virus contains at least one mutation or group of mutations selected from c.4969G>A, c.3334C>T, and a group of at least six of the mutations c.315G>A; c.1498A>G; c.1660C>T; c.3430C>T; c.3528A>G; c.4144C>T; c.4248C>T; and c.6228C>T. These mutated genomes encode one or more mutations selected from R1637H in the 186 kDa readthrough replication protein, A1092V in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and at least six mutations selected from G86S, E480G, S534F, A1124V, N1157D, P1362L, P1397S in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and the A156V mutation in the coat protein.