Abstract: Provided are an interaction method and apparatus in a live streaming room, a device, and a storage medium. The method comprises: in response to a trigger operation for a preset drawing entry on a live streaming room page, jumping to a graphic drawing page from the live streaming room page, a drawing trajectory set for a preset object being displayed on the graphical drawing page; when a drawing stroke on the graphic drawing page is received, matching the drawing stroke with the drawing trajectory; and if it is determined that the drawing stroke is successfully matched with the drawing trajectory, displaying prompt information about successful participation in a preset activity, the preset activity and the preset drawing entry having a correspondence.

Abstract: A molecular sieve of MFI structure has a ratio of n(SiO2)/n(Al2O3) of more than 15 and less than 70. It has a content of phosphorus of 1-15 wt %, calculated as P2O5 and based on the dry weight of the molecular sieve and a content of the supported metal in the molecular sieve 1-10 wt % based on the oxide of the supported metal and the dry weight of the molecular sieve. The supported metal is one or two selected from lanthanum and cerium. The volume of mesopores in the molecular sieve represents 40-70% by volume of the total pore volume of the molecular sieve by volume, measured by a nitrogen adsorption BET specific surface area method, and the volume of mesopores means the pore volume of the pores having a diameter of more than 2 nm and less than 100 nm.

Abstract: A rare earth- and phosphorus-containing molecular sieve of MFI structure rich in mesopores has a ratio of n(SiO2)/n(Al2O3) of more than 15 and less than 70. The molecular sieve has a content of phosphorus of 1-15 wt %, calculated as P2O5 and based on the dry weight of the molecular sieve. The content of the supported metal in the molecular sieve is 1-10 wt % supported metal M1 and 0.1-5 wt % supported metal M2 based on the oxide of the supported metal and the dry weight of the molecular sieve. The supported metal M1 is one or two selected from lanthanum and cerium, and the supported metal M2 is one selected from iron, cobalt, nickel, copper, manganese, zinc, tin, bismuth and gallium; the volume of mesopores in the molecular sieve represents 40-70% by volume of the total pore volume of the molecular sieve by volume.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: A lighting apparatus includes a solar plate, a battery, a driver module, a light source, a lens tube, a main housing and a plugging bar. The solar plate converts a sunlight to an electricity. The battery stores the electricity. The driver module converts the electricity stored in the battery to a driving current. The solar plate is placed on a top side of the driver module and the light source is placed on a bottom side of the driver module. The lens tube has a top opening and a bottom opening. The top opening has a larger size than the bottom opening. The top opening is closer to the light source than the bottom opening. A light of the light source is emitted via a lateral lens surface of the lens tube between the top opening and the bottom opening.

Abstract: Among other things, the present disclosure provides compositions and methods for analysis of cytosine modifications in nucleic acid sequences. In some embodiments, the present disclosure provides subtraction-free methods for detection of cytosine modifications in one or more nucleic acid sequences.

Abstract: A method for suppressing magnetizing inrush current of the transformer with flux linkage control includes connecting a small-capacity direct current/alternating current (DC/AC) converter with the secondary winding or auxiliary winding of transformer, detecting the primary side phase voltage before closing load, inducing the core flux linkage reference according to the relationship between the winding voltage and core flux linkage. The core flux linkage closed-loop PI control system is constructed to control the converter voltage in the synchronous coordinate, then the core flux linkage can track its reference with no static error, thus the sinusoidal flux linkage with 90-degree difference from the grid voltage can be pre-established in the core before no-load closing. By these methods, no matter when the main transformer closes, the core flux linkage is always in the steady state, and the magnetizing inrush current can be eliminated completely.

Abstract: The present disclosure provides a bisulfite-free, long-read, base-resolution method named long-read TAPS (lrTAPS) for detecting 5-Methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in a nucleic acid sequence. lrTAPS comprises mild enzymatic and chemical reactions to detect 5mC and 5hmC, the two major epigenetic marks found in the mammalian genome, quantitatively at base-resolution without affecting unmodified cytosine.

Abstract: A magnetic integrated hybrid distribution transformer includes a main transformer, a series isolation transformer and a converter, wherein: an iron core includes an iron beam unit, an iron yoke unit and a leakage magnetic core unit. The main transformer includes secondary windings, primary windings and control windings all of which are layer-windings and wound around main transformer iron beams. The series isolation transformer includes converter side windings and grid side windings all of which are pancake-windings and wound around isolation transformer iron beams. The converter side windings and the control windings are respectively connected with the converter by the star connection with neutral point. Leakage magnetic cores are respectively inserted between the primary windings and the control windings or between the converter side windings and the grid side windings, so as to achieve magnetic integration design of the transformer and output connection inductor of the converter.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: This disclosure provides methods for cost-effective bisulfite-free identification in DNA, including whole genomic DNA, of the locations of one or more of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine. The methods described herein are based on the conversion of modified cytosine (5mC, 5hmC, 5fC, 5caC) to dihydrouracil (DHU), for example by TET-assisted pyridine borane treatment, followed by endonuclease cleavage of the DHU, and identification of the cleavage site, which corresponds to the location of the modified cytosine.

Abstract: A method for suppressing magnetizing inrush current of the transformer with flux linkage control includes connecting a small-capacity direct current/alternating current (DC/AC) converter with the secondary winding or auxiliary winding of transformer, detecting the primary side phase voltage before closing load, inducing the core flux linkage reference according to the relationship between the winding voltage and core flux linkage. The core flux linkage closed-loop PI control system is constructed to control the converter voltage in the synchronous coordinate, then the core flux linkage can track its reference with no static error, thus the sinusoidal flux linkage with 90-degree difference from the grid voltage can be pre-established in the core before no-load closing. By these methods, no matter when the main transformer closes, the core flux linkage is always in the steady state, and the magnetizing inrush current can be eliminated completely.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: An attenuated strain of cucumber green mottle mosaic virus (CGMMV) is useful to protect cucumber plants from infection with the wild-type infectious CGMMV strain. The genome of the attenuated virus contains at least one mutation or group of mutations selected from c.4969G>A, c.3334C>T, and a group of at least six of the mutations c.315G>A; c.1498A>G; c.1660C>T; c.3430C>T; c.3528A>G; c.4144C>T; c.4248C>T; and c.6228C>T. These mutated genomes encode one or more mutations selected from R163711 in the 186 kDa readthrough replication protein, A1092V in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and at least six mutations selected from G86S, E480G, S534F, A1124V, N1157D, P1362L, P1397S in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and the A156V mutation in the coat protein.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: A method for preparing titanium-containing molecular sieves include the following steps: irradiating a mixed solution containing a silicon source, a template and a titanium source by a light source containing ultraviolet light before crystallization, and then subjecting the mixed solution to crystallization and post-treatment to obtain the titanium-containing molecular sieve. In the method for preparing titanium-containing molecular sieve of the present invention, the mixed solution containing the silicon source, the template and the titanium source is treated with the ultraviolet light, and free radicals generated by the ultraviolet light can effectively dissociate titanium oligomers and accelerate the hydrolysis of the silicon source, and thus match the hydrolysis rate of a silicon-titanium precursor, thereby preventing a titanium monomer from self-polymerization to form non-framework titanium.

Abstract: An attenuated strain of cucumber green mottle mosaic virus (CGMMV) is useful to protect cucumber plants from infection with the wild-type infectious CGMMV strain. The genome of the attenuated virus contains at least one mutation or group of mutations selected from c.4969G>A, c.3334C>T, and a group of at least six of the mutations c.315G>A; c.1498A>G; c.1660C>T; c.3430C>T; c.3528A>G; c.4144C>T; c.4248C>T; and c.6228C>T. These mutated genomes encode one or more mutations selected from R163711 in the 186 kDa readthrough replication protein, A1092V in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and at least six mutations selected from G86S, E480G, S534F, A1124V, N1157D, P1362L, P1397S in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and the A156V mutation in the coat protein.

Abstract: This disclosure provides methods for bisulfite-free identification in a nucleic acid sequence of the locations of 5-methyl-cytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine.

Abstract: An attenuated strain of cucumber green mottle mosaic virus (CGMMV) is useful to protect cucumber plants from infection with the wild-type infectious CGMMV strain. The genome of the attenuated virus contains at least one mutation or group of mutations selected from c.4969G>A, c.3334C>T, and a group of at least six of the mutations c.315G>A; c.1498A>G; c.1660C>T; c.3430C>T; c.3528A>G; c.4144C>T; c.4248C>T; and c.6228C>T. These mutated genomes encode one or more mutations selected from R1637H in the 186 kDa readthrough replication protein, A1092V in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and at least six mutations selected from G86S, E480G, S534F, A1124V, N1157D, P1362L, P1397S in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and the A156V mutation in the coat protein.