Abstract: A CRISPR electrochemical biosensing system (E-CRISPR) for detection of analytes includes a disposable, micro-fabricated three-electrode sensor that includes a working electrode, a counter electrode, a reference electrode, and a nonspecific ssDNA reporter with an electrochemical tag for signal transduction tethered to a surface of the working electrode; and a Cas12a-crRNA duplex that is designed to specifically recognize and cleave target nucleic acid strand based on the protospacer adjacent motif (PAM) sequence of the target and crRNA sequence, wherein the PAM recognition depends on specific 5? TTTN nucleic acid sequence located at an opposite strand of a recognition strand, and wherein only upon the recognition of the PAM sequence by the Cas protein, the Cas protein, acting as a DNA helicase, unwinds the target DNA.

Abstract: Provided is gRNA specifically targeting ?4GalNT2 gene. The gRNA specifically binds to the nucleotide sequence shown in any one of SEQ ID NOs. 1 and 2. Also provided are an animal model constructed using the gRNA, and an application thereof in the field of biomedicine.

Abstract: A sensor for the detection of lead includes a substrate, a working electrode formed on a surface of the substrate, a counter electrode formed on the surface of the substrate, a dielectric layer covering a portion of the working electrode and counter electrode and defining an aperture exposing other portions of the working electrode and counter electrode. The working electrode includes a metalized film with working surface and a monolayer (or bilayer) of bismuth deposited on the working surface by underpotential deposition.

Abstract: Provided is an SgRNA combination, comprising an SgRNA specifically targeting the GGTA1 gene, an SgRNA specifically targeting the CMAH gene and an SgRNA specifically targeting the ?4GalNT2 gene. Also provided is a CRISPR/Cas9 vector combination, comprising a GGTA1-CRISPR/Cas9 vector, a CMAH-CRISPR/Cas9 vector and a ?4GalNT2-CRISPR/Cas9 vector. Also provided is an applicaton of the CRISPR/Cas9 vector combination in knocking out the GGTA1 gene, the CMAH gene and the ?4GalNT2 gene. The knockout rates of the three genes with the specifically targeted SgRNA sequences are respectively 56%, 63%, and 41%. A three genes knockoutpig can be obtained, wherein the three genes related to immune rejectionare knocked out, and heart valves of said pig can be acquired.

Abstract: A sensor for the detection of analyte includes a substrate, a working electrode and counter electrode formed on a surface of the substrate, and an antibody bioconjugated to a surface of an exposed portion of the working electrode.

Abstract: A blood product may be derived from a gene knockout pig and used in, e.g., medical applications. The binding of the blood product to immunoglobulin in human serum is reduced, and the blood product can have an effect on overcoming hyperacute immune rejection. The blood product may be derived from a gene knockout pig, wherein a GGTA1 gene, a CMAH gene, and/or a ?4GalNT2 gene of the gene knockout pig are knocked out, wherein one or more nucleotides in the ?4GalNT2 gene encoding one or more amino acids in exon 8 are deleted such that the ?4GalNT2 gene is knocked out.

Abstract: A CRISPR electrochemical biosensing system (E-CRISPR) for detection of analytes includes a disposable, micro-fabricated three-electrode sensor that includes a working electrode, a counter electrode, a reference electrode, and a nonspecific ssDNA reporter with an electrochemical tag for signal transduction tethered to a surface of the working electrode; and a Cas12a-crRNA duplex that is designed to specifically recognize and cleave target nucleic acid strand based on the protospacer adjacent motif (PAM) sequence of the target and crRNA sequence, wherein the PAM recognition depends on specific 5? TTTN nucleic acid sequence located at an opposite strand of a recognition strand, and wherein only upon the recognition of the PAM sequence by the Cas protein, the Cas protein, acting as a DNA helicase, unwinds the target DNA,

Abstract: A sensor for the detection of AMACR and/or PSA includes a substrate, a working electrode and counter electrode formed on a surface of the substrate, and an anti-AMACR antibody and/or anti-PSA antibody bioconjugated to a surface of an exposed portion of the working electrode.

Abstract: A sensor for the detection of 17?-estradiol in a sample includes a substrate, a working electrode and counter electrode formed on a surface of the substrate, and an anti-estrogen receptor functionalized or chemically functionalized to a surface of an exposed portion of the working electrode.

Abstract: Provided is an SgRNA combination, comprising an SgRNA specifically targeting the GGTA1 gene, an SgRNA specifically targeting the CMAH gene and an SgRNA specifically targeting the ?4GalNT2 gene. Also provided is a CRISPR/Cas9 vector combination, comprising a GGTA1-CRISPR/Cas9 vector, a CMAH-CRISPR/Cas9 vector and a ?4GalNT2-CRISPR/Cas9 vector. Also provided is an applicaton of the CRISPR/Cas9 vector combination in knocking out the GGTA1 gene, the CMAH gene and the ?4GalNT2 gene. The knockout rates of the three genes with the specifically targeted SgRNA sequences are respectively 56%, 63%, and 41%. A three genes knockoutpig can be obtained, wherein the three genes related to immune rejectionare knocked out, and heart valves of said pig can be acquired.

Abstract: A sensor for the detection of lead includes a substrate, a working electrode formed on a surface of the substrate, a counter electrode formed on the surface of the substrate, a dielectric layer covering a portion of the working electrode and counter electrode and defining an aperture exposing other portions of the working electrode and counter electrode. The working electrode includes a metalized film with working surface and a monolayer (or bilayer) of bismuth deposited on the working surface by underpotential deposition.

Abstract: A sensor for the detection of analyte includes a substrate, a working electrode and counter electrode formed on a surface of the substrate, and an antibody bioconjugated to a surface of an exposed portion of the working electrode.

Abstract: A non-enzymatic sensor for the detection of glucose includes a substrate, a working electrode formed on a surface of the substrate; a counter electrode formed on the surface of the substrate; a dielectric layer covering a portion of the working electrode and counter electrode and defining an aperture exposing other portions of the working electrode and counter electrode; and a cuprous oxide (Cu2O) film electrodeposited on the working electrode.

Abstract: A sensor for the detection of GPC-1 in a sample includes a substrate, a working electrode and counter electrode formed on a surface of the substrate, and an anti-GPC-1 antibody functionalized or chemically functionalized to a surface of an exposed portion of the working electrode.

Abstract: A sensor for the detection of ?-amyloid 42, T-Tau, and/or P-Tau in a sample includes a substrate, a working electrode and counter electrode formed on a surface of the substrate, and an anti-?-amyloid 42 antibody, anti-T-Tau antibody, and/or anti-P-Tau antibody functionalized or chemically functionalized to a surface of an exposed portion of the working electrode.

Abstract: In a computer network comprising a stack device and a plurality of network devices, the plurality of network devices are each connected to the stack device via a respective aggregation link comprising a plurality of member links and the stack device comprises a first stack member and a second stack member connected to the first stack member via a stack link. A correspondence is formed between a first route and the first stack member. The first route is used by the first stack member for forwarding traffic from a first network device to a second network device of the plurality, and comprises a member link via which the traffic is forwarded from the first stack member.

Abstract: In a computer network comprising a stack device and a plurality of network devices, the plurality of network devices are each connected to the stack device via a respective aggregation link comprising a plurality of member links and the stack device comprises a first stack member and a second stack member connected to the first stack member via a stack link. A correspondence is formed between a first route and the first stack member. The first route is used by the first stack member for forwarding traffic from a first network device to a second network device of the plurality, and comprises a member link via which the traffic is forwarded from the first stack member.

Abstract: A variable near-null compensator for measuring aspheric surfaces by subaperture stitching includes a pair of counter-rotating CGH phase plates, each of the phase plates having a phase function including two terms Z5 and Z7 of Zernike polynomials. The phase plates are mounted on a pair of precision rotary center-through tables, wherein rotational axes of the pair of precision rotary center-through tables coincide with the optical axes of the phase plates. A figure metrology apparatus includes a wavefront interferometer, the test mirror mount, the near-null compensator and the mechanical adjustment components therefor. The optical axis of the near-null compensator coincides with the optical axis of the interferometer. A method for measuring aspheric surfaces by subaperture stitching includes the steps of mounting the test mirror, measuring the subapertures with the figure metrology apparatus, and finally processing the data by stitching.

Abstract: A variable near-null compensator for measuring aspheric surfaces by subaperture stitching includes a pair of counter-rotating CGH phase plates, each of the phase plates having a phase function including two terms Z5 and Z7 of Zernike polynomials. The phase plates are mounted on a pair of precision rotary center-through tables, wherein rotational axes of the pair of precision rotary center-through tables coincide with the optical axes of the phase plates. A figure metrology apparatus includes a wavefront interferometer, the test mirror mount, the near-null compensator and the mechanical adjustment components therefor. The optical axis of the near-null compensator coincides with the optical axis of the interferometer. A method for measuring aspheric surfaces by subaperture stitching includes the steps of mounting the test mirror, measuring the subapertures with the figure metrology apparatus, and finally processing the data by stitching.

Abstract: The production of genetically modified animals, in which the genetic modifications are engineered in somatic cells cultured in vitro by gene targeting, is described. Genetically modified cells may then used as nuclear donors to produce, inter alia, live animals. The methods described can also be used to validate loci in animal chromosomes which are suitable sites for transgene addition to cells.