Patents by Inventor Yii Leng Chua

Yii Leng Chua has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10544465
    Abstract: The present invention relates to methods and tool for amplification and detection of different NRAS isotypes.
    Type: Grant
    Filed: October 9, 2014
    Date of Patent: January 28, 2020
    Assignee: VELA OPERATIONS SINGAPORE PTE. LTD.
    Inventors: Louis Welebob, Yii Leng Chua, Tatiana Ivanova, Sameer Phalke
  • Publication number: 20180257002
    Abstract: A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilise capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.
    Type: Application
    Filed: October 20, 2017
    Publication date: September 13, 2018
    Inventor: Yii Leng Chua
  • Patent number: 9821248
    Abstract: A method for obtaining a liquid from a porous solid phase is described. The method comprises forming a liquid seal at a first end of a porous solid phase to which a liquid is bound, wherein liquid of the liquid seal is immiscible with the liquid bound to the solid phase, and applying a pressure differential across the porous solid phase to cause the immiscible liquid to move through the porous solid phase towards a second end of the porous solid phase, thereby displacing the liquid bound to the porous solid phase towards the second end and releasing this liquid from the second end. Recovery of liquid from the solid phase using such methods is increased compared with corresponding methods in which no liquid seal is formed. In preferred embodiments, the liquid used to form the liquid seal is a mineral oil. The methods have particular application in nucleic acid extractions which utilize capture of nucleic acid to a solid phase. Kits and apparatus for performing the methods are also described.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: November 21, 2017
    Assignee: Cambridge Enterprise Limited
    Inventor: Yii Leng Chua
  • Publication number: 20170037394
    Abstract: A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.
    Type: Application
    Filed: July 11, 2016
    Publication date: February 9, 2017
    Inventors: Yii Leng CHUA, Allyson Victoria RITCHIE
  • Patent number: 9422543
    Abstract: A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.
    Type: Grant
    Filed: August 7, 2009
    Date of Patent: August 23, 2016
    Assignee: CAMBRIDGE ENTERPRISE LIMITED
    Inventors: Yii Leng Chua, Allyson Victoria Ritchie
  • Publication number: 20110257386
    Abstract: A method for isolating a nucleic acid comprises: binding the nucleic acid to a solid phase at a first pH in the presence of a binding buffer, washing the bound nucleic acid with a wash solution, and eluting the nucleic acid from the solid phase at a second pH which is higher than the first pH. The wash solution comprises a buffer with a buffering range that encompasses a pH that is higher than the first pH, and the wash solution is at a pH that is within a buffering range of the binding buffer but lower than the buffering range of the buffer of the wash solution. Solutions, compositions, and kits for use in the methods are described.
    Type: Application
    Filed: August 7, 2009
    Publication date: October 20, 2011
    Inventors: Yii Leng Chua, Allyson Victoria Ritchie