Abstract: A transgenic method of obtaining blue flowers by catalyzing glutamine to synthesize indigo. The steps include: 1) respectively cloning a Sfp gene encoding phosphopantetheinyl transferase and a bpsA gene encoding indigo synthase downstream of a plant promoter in a plant-promoter-containing plasmid; 2) amplifying the obtained plasmid in E. coli and then transferring the same to Agrobacterium tumefaciens; and 3) transferring DNA containing Sfp and bpsA into a plant. The blue flowers produced by the present invention have various characteristics of natural flowers, being fresh, flower-scented, non-color-fading, and non-toxic. The transgene-encoded enzyme and the produced indigo are not in the vacuole and are not affected by the low pH of the plant vacuole, thereby resulting in a pure blue color. The precursor of the blue matter, i.e., the substrate of the enzyme, is glutamine, which is abundant in plants.

Abstract: A transgenic method of obtaining blue flowers by catalyzing glutamine to synthesize indigo is provided. The steps thereof comprise: 1) respectively cloning a Sfp gene encoding phosphopantetheinyl transferase and a bpsA gene encoding indigo synthase downstream of a plant promoter in a plant-promoter-containing plasmid; 2) amplifying the obtained plasmid in E. coli and then transferring the same to Agrobacterium tumefaciens; and 3) transferring DNA containing Sfp and bpsA into a plant. The blue flowers produced by the present invention have various characteristics of natural flowers, being fresh, flower-scented, non-color-fading, and non-toxic. The transgene-encoded enzyme and the produced indigo are not in the vacuole and are not affected by the low pH of the plant vacuole, thereby resulting in a pure blue color. The precursor of the blue matter, i.e., the substrate of the enzyme, is glutamine, which is abundant in plants.

Abstract: Provided are a xanthine amide hydrolase and the use thereof, particularly the use thereof for treating gout.

Abstract: Provided are a 4-ureido-5-carboxyl-imidazole-amide hydrolase and use thereof, particularly use in the treatment of gout.