Abstract: The present disclosure provides an N protein epitope mutation marker for preparing an epitope deletion-marked vaccine strain of type II porcine reproductive and respiratory syndrome virus (PRRSV) and use thereof, belonging to the technical field of biological products. In the mutation marker, one or more amino acids are mutated based on an epitope sequence at positions 92 to 103 of a C-terminal of an N protein of the type II PRRSV; and the epitope mutation marker has an amino acid sequence shown in SEQ ID NO: 1, where X1 is selected from the group consisting of T, P, and A; and X2 is selected from the group consisting of V and A.

Abstract: The present invention discloses an ecological seawall water close side embankment slope drainage structure and a construction method thereof. The ecological seawall water close side embankment slope drainage structure includes a seawall, and further includes a wave dissipation ridge and water collecting wells, wherein the wave dissipation ridge is disposed at an upper side of the seawall, a drainage blind ditch is formed in an upper surface of the seawall, and the drainage blind ditch is matched with the wave dissipation ridge; the water collecting wells are distributed at a lower side of the drainage blind ditch at equal intervals, and lower end portions of the water collecting wells communicate with drainage concealed pipes; and pipe seats are disposed at a lower end portion of an inclined plane of the seawall, lower end portions of the drainage concealed pipes are fixedly connected onto the pipe seats, and flap valves are rotationally connected to lower end surfaces of the drainage concealed pipes.

Abstract: The present invention discloses a water intake pipeline structure passing through a soft foundation embankment below a flood level and a construction method. The water intake pipeline structure comprises: pipelines, a gate valve well, a concrete pipe bed, a high-pressure jet grouting pile continuous wall, cement deep mixing piles, geogrids, backfilling clay, a clay bound macadam and concrete key walls. After the embankment is broken and excavated to reach a pipeline embankment-passing design elevation, the cement deep mixing piles are fully disposed in a plum blossom type, and the high-pressure jet grouting pile continuous wall is disposed under the gate valve well. A plain concrete cushion layer, the gate valve well, the concrete key walls and the concrete pipe bed are poured, the water intake pipelines are installed, and the pipelines behind the gate valve well are wrapped with concrete. The geogrids are placed during clay backfilling, and layered soil filling is performed.

Abstract: The present invention discloses an ecological seawall water close side embankment slope drainage structure and a construction method thereof. The ecological seawall water close side embankment slope drainage structure includes a seawall, and further includes a wave dissipation ridge and water collecting wells, wherein the wave dissipation ridge is disposed at an upper side of the seawall, a drainage blind ditch is formed in an upper surface of the seawall, and the drainage blind ditch is matched with the wave dissipation ridge; the water collecting wells are distributed at a lower side of the drainage blind ditch at equal intervals, and lower end portions of the water collecting wells communicate with drainage concealed pipes; and pipe seats are disposed at a lower end portion of an inclined plane of the seawall, lower end portions of the drainage concealed pipes are fixedly connected onto the pipe seats, and flap valves are rotationally connected to lower end surfaces of the drainage concealed pipes.