Abstract: A neural network parameter quantization method includes obtaining a parameter of each neuron in a to-be-quantized model to obtain a parameter set, clustering parameters in the parameter set to obtain types of classified data, and quantizing each type of classified data in the types of classified data to obtain at least one type of quantization parameter, where the at least one type of quantization parameter is used to obtain a compression model, and precision of the at least one type of quantization parameter is lower than precision of a parameter in the to-be-quantized model.

Abstract: A trailed straw biomass granulator includes a first drive device and a forming device. The forming device includes a driving gear and a forming gear. The driving gear is evenly distributed with involute drive teeth along a circumference. A storage groove is formed between every two adjacent drive teeth. The driving gear includes an output hole in an axial direction, and an auger is arranged inside the output hole. A forming hole is defined between two adjacent drive teeth, and the forming hole penetrates into the output hole and the storage groove. The forming gear includes a base body, multiple involute sliding teeth and multiple reset springs, and the sliding teeth are evenly distributed on the peripheral side of the base body in a circumferential direction. The base body includes multiple sliding grooves in one-to-one correspondence with the sliding teeth, which are integrally formed with a slide block.

Abstract: A trailed straw biomass granulator includes a first drive device and a forming device. The forming device includes a driving gear and a forming gear. The driving gear is evenly distributed with involute drive teeth along a circumference. A storage groove is formed between every two adjacent drive teeth. The driving gear includes an output hole in an axial direction, and an auger is arranged inside the output hole. A forming hole is defined between two adjacent drive teeth, and the forming hole penetrates into the output hole and the storage groove. The forming gear includes a base body, multiple involute sliding teeth and multiple reset springs, and the sliding teeth are evenly distributed on the peripheral side of the base body in a circumferential direction. The base body includes multiple sliding grooves in one-to-one correspondence with the sliding teeth, which are integrally formed with a slide block.

Abstract: The field of the invention is cellular and molecular biology and stem cells. Specifically, the disclosure is directed to a formulation for harvesting and passaging single cell human pluripotent stem cells comprising: (i) 1 mM to about 30 mM sodium citrate; (ii) a salt comprising 10 mM to 170 mM KCl or NaCl; and (iii) Ca2+/Mg2+-free Dulbecco's phosphate buffered saline (DPBS), wherein said formulation has an osmolarity of about 100 mOsmol/liter to about 350 mOsmol/liter. The formulation can be used for serial passaging and dislodging of pluripotent stem cells attached to 2D tissue culture vessels or grown in 3D suspension culture (small scale and large scale bioreactors) or any other application where passaging in the form of single cell population of stem cells is needed.

Abstract: Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and are able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyotype after passaged with the formulations for extended period of time.

Abstract: Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and arc able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyotype after passaged with the formulations for extended period of time.

Abstract: Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and are able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyo-type after passaged with the formulations for extended period of time.

Abstract: Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and are able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyo-type after passaged with the formulations for extended period of time.

Abstract: The invention relates to a method for promoting the adhesion of cells to a substrate to which these cells usually have no or only low affinity, wherein the adhesion of the cells to the substrate is promoted by supplying the cells with the non-muscle myosin II inhibitor Blebbistatin so as to enable the cells to attach to surfaces to which they otherwise would not have sufficient affinity. Surprisingly, supplying the cells with the inhibitor enhances the capability of these cells to attach to surfaces to which they usually have no or only low affinity, for example, PTFE (Teflon®). The invention further concerns uses of the non-muscle myosin II inhibitor Blebbistatin and devices having at least one surface which is coated with cells that have no or only low affinity to said surface.

Abstract: A protectant mixture for use in preserving biological materials comprising at least one polyhydroxy compound, and phosphate ions; a preservation medium comprising a biological material, at least one polyhydroxy compound, and phosphate ions; methods of preserving the preservation medium; and the resulting preserved biological material composition.

Abstract: A protectant mixture for use in preserving biological materials comprising at least one polyhydroxy compound, and phosphate ions; a preservation medium comprising a biological material, at least one polyhydroxy compound, and phosphate ions; methods of preserving the preservation medium; and the resulting preserved biological material composition.