Abstract: In a method for biosensor analysis, a sample is tested in multiple stages using a biosensor and a test chip thereof to enable easy and convenient handling of the biosensor and accurate analyte measurement with the biosensor. The test chip of the biosensor has specially designed biochemical reaction zone, in which immobilized enzymes and high-molecular bonding agent are applied, so that even a very small liquid sample may be quickly introduced into and absorbed at the biochemical reaction zone to biochemically react with the enzymes on the test chip. With the multi-stage testing method and electronic circuits of the biosensor for logic determination, the biosensor may have increased accuracy.