Abstract: A method and system for strategically solving an innovative design problem are provided. A target analysis tool is determined according to a to-be-solved problem. An initial model is generated based on the target analysis tool. A target model is determined according to an interaction relationship among elements in the initial model and the target analysis tool. A problem characterization result of the target model is generated according to a preset interaction relationship. Retrieval information is generated according to the problem characterization result, based on which the retrieval is performed in a preset database to obtain target data. Different analysis tools can be used to analyze different to-be-solved problems. The retrieval is performed based on the problem characterization result to obtain the solution to the to-be-solved problem.

Abstract: A method and system for strategically solving an innovative design problem are provided. A target analysis tool is determined according to a to-be-solved problem. An initial model is generated based on the target analysis tool. A target model is determined according to an interaction relationship among elements in the initial model and the target analysis tool. A problem characterization result of the target model is generated according to a preset interaction relationship. Retrieval information is generated according to the problem characterization result, based on which the retrieval is performed in a preset database to obtain target data. Different analysis tools can be used to analyze different to-be-solved problems. The retrieval is performed based on the problem characterization result to obtain the solution to the to-be-solved problem.

Abstract: Certain embodiments are directed to detection methods and methods of identifying detection targets for sensitive and specific laboratory tests to diagnose and monitor rickettsial infections during the earliest stages of the disease and to assess the degree of illness.

Abstract: A quick-detachable connecting device with a simplified thread structure includes a top nozzle, a connecting tube sleeve, a locking tube and a locking piece. The top nozzle is provided with a through hole, in which the connecting tube sleeve is assembled. The locking tube is accommodated in the through hole. An outer peripheral surface of the locking tube is provided with a second stopping structure, and an inner peripheral surface of the connecting tube sleeve is provided with a first stopping structure fitting the second stopping structure. The locking piece is movably arranged on the locking tube for circumferentially limiting or releasing the locking tube. A connection method using such connecting device is also provided.

Abstract: A quick-detachable connecting device with a simplified thread structure includes a top nozzle, a connecting tube sleeve, a locking tube and a locking piece. The top nozzle is provided with a through hole, in which the connecting tube sleeve is assembled. The locking tube is accommodated in the through hole. An outer peripheral surface of the locking tube is provided with a second stopping structure, and an inner peripheral surface of the connecting tube sleeve is provided with a first stopping structure fitting the second stopping structure. The locking piece is movably arranged on the locking tube for circumferentially limiting or releasing the locking tube. A connection method using such connecting device is also provided.

Abstract: Certain embodiments are directed to detection methods and methods of identifying detection targets for sensitive and specific laboratory tests to diagnose and monitor rickettsial infections during the earliest stages of the disease and to assess the degree of illness.

Abstract: DNA aptamers are high affinity ligands selected by genetic enrichment techniques to bind to specific protein targets. Because these represent chemically stable and reproducible molecules, they have application as affinity reagents and/or therapeutic drugs to affect the target protein's actions. NF-kB is an important mediator of the innate immune response and mediator of tissue inflammation. Although RNA and double stranded DNA aptamers have been identified to bind to the NF-kB family of proteins, the present invention represents the first identification of single stranded DNA aptamers that recognize NFkB RelA. The aptamers disclosed herein bind to several distinct regions of RelA and may be useful to antagonize the DNA binding of RelA as an inhibitor of cellular inflammation, visualize the location or amount of RelA in tissues from pathological conditions, or to quantitatively measure the activated state of RelA by affinity binding.

Abstract: DNA aptamers are high affinity ligands selected by genetic enrichment techniques to bind to specific protein targets. Because these represent chemically stable and reproducible molecules, they have application as affinity reagents and/or therapeutic drugs to affect the target protein's actions. NF-kB is an important mediator of the innate immune response and mediator of tissue inflammation. Although RNA and double stranded DNA aptamers have been identified to bind to the NF-kB family of proteins, the present invention represents the first identification of single stranded DNA aptamers that recognize NFkB RelA. The aptamers disclosed herein bind to several distinct regions of RelA and may be useful to antagonize the DNA binding of RelA as an inhibitor of cellular inflammation, visualize the location or amount of RelA in tissues from pathological conditions, or to quantitatively measure the activated state of RelA by affinity binding.

Abstract: DNA aptamers are high affinity ligands selected by genetic enrichment techniques to bind to specific protein targets. Because these represent chemically stable and reproducible molecules, they have application as affinity reagents and/or therapeutic drugs to affect the target protein's actions. NF-kB is an important mediator of the innate immune response and mediator of tissue inflammation. Although RNA and double stranded DNA aptamers have been identified to bind to the NF-kB family of proteins, the present invention represents the first identification of single stranded DNA aptamers that recognize NFkB RelA. The aptamers disclosed herein bind to several distinct regions of RelA and may be useful to antagonize the DNA binding of RelA as an inhibitor of cellular inflammation, visualize the location or amount of RelA in tissues from pathological conditions, or to quantitatively measure the activated state of RelA by affinity binding.

Abstract: DNA aptamers are high affinity ligands selected by genetic enrichment techniques to bind to specific protein targets. Because these represent chemically stable and reproducible molecules, they have application as affinity reagents and/or therapeutic drugs to affect the target protein's actions. NF-kB is an important mediator of the innate immune response and mediator of tissue inflammation. Although RNA and double stranded DNA aptamers have been identified to bind to the NF-kB family of proteins, the present invention represents the first identification of single stranded DNA aptamers that recognize NFkB RelA. The aptamers disclosed herein bind to several distinct regions of RelA and may be useful to antagonize the DNA binding of RelA as an inhibitor of cellular inflammation, visualize the location or amount of RelA in tissues from pathological conditions, or to quantitatively measure the activated state of RelA by affinity binding.