Abstract: A multi-scale aggregation pattern analysis method for a complex traffic network is provided, which belongs to the field of the highway traffic network. Firstly, an adjacency matrix, a position attribute matrix, a distance weight matrix, a road grade matrix, and a time-phased traffic congestion degree matrix of a highway traffic network are calculated; secondly, a weight influence factor of the road network is incorporated based on a PageRank algorithm to determine order of critical nodes; finally, a two-dimensional decision diagram is drawn by two indicators: order of critical nodes and a shortest path distance. A new weighting matrix which accords with the actual situation of the road network is obtained by incorporating a position weight matrix, a distance weight matrix, a road grade weight matrix and a dynamic traffic congestion degree weight matrix based on a similarity matrix of the spectral clustering.

Abstract: Disclosed is a fusion protein of a mutated recombinant single-chain human coagulation factor VIII (FVIII), a preparation method therefor, and a use thereof. The fusion protein sequentially comprises, from an N-terminus to a C-terminus, a mutated single-chain human FVIII having a partially deleted B-domain, a flexible peptide linker, at least one rigid unit of a carboxyl-terminal peptide of a human chorionic gonadotropin beta subunit, and a half-life prolonging moiety (preferably an IgG Fc variant). The fusion protein has a similar biological activity to a recombinant FVIII, a prolonged active half life in vivo, and better stability in vitro and in vivo, and thus improves the pharmacokinetics and efficacy of the fusion protein.

Abstract: Provided is a tetravalent homodimeric bispecific antibody molecule simultaneously targeting an immune effector cell antigen CD3 and a tumor-associated antigen, wherein the bispecific antibody molecule contains, in order from N-terminus to C-terminus, a first single chain Fv, a second single chain Fv and a Fc fragment; wherein the first single chain Fv can specifically bind to the tumor-associated antigen, the second single chain Fv can specifically bind to CD3, and the first and the second single chain Fvs are connected by a linker peptide, while the second single chain Fv and the Fc fragment are directly connected or connected by a linker peptide; and the Fc fragment does not have effector functions such as CDC, ADCC and ADCP.