Patents by Inventor Yinhua Zhang
Yinhua Zhang has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11008629Abstract: Kits and methods are provided for performing multiplex LAMP reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting the viral nucleic acid target.Type: GrantFiled: December 15, 2020Date of Patent: May 18, 2021Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Gregory Patton, Guoping Ren, Zhiru Li, Nicole Nichols
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Patent number: 10968493Abstract: Kits and methods are provided for performing multiplex LAMP reactions. These kits and methods are directed to specific and sensitive methods of target nucleic acid detection and more specifically pathogen diagnostics such as detection of Coronavirus. The kits and methods utilize a plurality of sets of oligonucleotide primers for targeting different template sequences in a single nucleic acid target. The kits and methods also include guanidium salts that enhance the sensitivity of the assay.Type: GrantFiled: July 24, 2020Date of Patent: April 6, 2021Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Gregory Patton, Guoping Ren, Zhiru Li, Nicole Nichols
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Patent number: 10934533Abstract: Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a reduction of bias. Methods of using the DNA polymerase variant are also described herein.Type: GrantFiled: October 10, 2019Date of Patent: March 2, 2021Assignee: New England Biolabs, Inc.Inventors: Jennifer Ong, Nathan Tanner, Yinhua Zhang, Yanxia Bei, Vladimir Potapov
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Publication number: 20190169683Abstract: Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and Tris buffer in the range of 1.5 mM Tris to 5 mM Tris or equivalent.Type: ApplicationFiled: May 12, 2017Publication date: June 6, 2019Applicant: New England Biolabs, Inc.Inventors: Yinhua Zhang, Nathan Tanner, Thomas C. Evans, Jr.
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Patent number: 9920305Abstract: Compositions and methods are provided for improved reverse transcriptases and their uses in reverse transcription where the improvement may include increased temperature, increased salt, increased activity and/or increased dUTP tolerance.Type: GrantFiled: October 16, 2014Date of Patent: March 20, 2018Assignee: New England Biolabs, Inc.Inventors: Yinhua Zhang, Thomas C. Evans, Jr.
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Patent number: 9580748Abstract: Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer.Type: GrantFiled: April 7, 2015Date of Patent: February 28, 2017Assignee: New England BioLabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Thomas C. Evans
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Publication number: 20150240293Abstract: Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer.Type: ApplicationFiled: April 7, 2015Publication date: August 27, 2015Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Nathan Tanner, Yinhua Zhang, Thomas C. Evans
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Patent number: 9074249Abstract: Compositions and methods are provided for quantitative detection of amplification products, the methods being suitable for multiplexing. A first oligonucleotide that includes a primer sequence for priming an amplification reaction and also is labeled with a fluorescent label or quencher is mixed with a second oligonucleotide which has a sequence suitable for hybridizing to a portion of the first oligonucleotide and has a fluorescent label if the first oligonucleotide has a quencher or a quencher if the first oligonucleotide has a fluorescent label; and a third nucleotide which includes some or all the primer sequence contained in the first oligonucleotide but is not labeled, the first and third oligonucleotide being combined in a molar ratio of 2.8 to 8.2.Type: GrantFiled: March 15, 2013Date of Patent: July 7, 2015Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Thomas C. Evans
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Patent number: 9074243Abstract: Compositions and methods are provided for quantitative detection of amplification products, the methods being suitable for multiplexing. A first oligonucleotide that includes a primer sequence for priming an amplification reaction and also is labeled with a fluorescent label or quencher is mixed with a second oligonucleotide which has a sequence suitable for hybridizing to a portion of the first oligonucleotide and has a fluorescent label if the first oligonucleotide has a quencher or a quencher if the first oligonucleotide has a fluorescent label; and a third nucleotide which includes some or all the primer sequence contained in the first oligonucleotide but is not labeled, the first and third oligonucleotide being combined in a molar ratio of 2.8 to 8.2.Type: GrantFiled: March 14, 2013Date of Patent: July 7, 2015Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Thomas C. Evans
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Patent number: 9034606Abstract: Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer.Type: GrantFiled: March 13, 2013Date of Patent: May 19, 2015Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Thomas C. Evans
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Publication number: 20150104790Abstract: Compositions and methods are provided for improved reverse transcriptases and their uses in reverse transcription where the improvement may include increased temperature, increased salt, increased activity and/or increased dUTP tolerance.Type: ApplicationFiled: October 16, 2014Publication date: April 16, 2015Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Yinhua Zhang, Thomas C. Evans, JR.
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Publication number: 20140057268Abstract: Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and a weak buffer of less than 1 mM Tris or equivalent or no buffer.Type: ApplicationFiled: March 13, 2013Publication date: February 27, 2014Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Nathan Tanner, Yinhua Zhang, Thomas C. Evans
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Publication number: 20140031248Abstract: Compositions and methods are provided for quantitative detection of amplification products, the methods being suitable for multiplexing. A first oligonucleotide that includes a primer sequence for priming an amplification reaction and also is labeled with a fluorescent label or quencher is mixed with a second oligonucleotide which has a sequence suitable for hybridizing to a portion of the first oligonucleotide and has a fluorescent label if the first oligonucleotide has a quencher or a quencher if the first oligonucleotide has a fluorescent label; and a third nucleotide which includes some or all the primer sequence contained in the first oligonucleotide but is not labeled, the first and third oligonucleotide being combined in a molar ratio of 2.8 to 8.2.Type: ApplicationFiled: March 14, 2013Publication date: January 30, 2014Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Nathan Tanner, Yinhua Zhang, Thomas C. Evans
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Publication number: 20130323738Abstract: Compositions and methods are provided for quantitative detection of amplification products, the methods being suitable for multiplexing. A first oligonucleotide that includes a primer sequence for priming an amplification reaction and also is labeled with a fluorescent label or quencher is mixed with a second oligonucleotide which has a sequence suitable for hybridizing to a portion of the first oligonucleotide and has a fluorescent label if the first oligonucleotide has a quencher or a quencher if the first oligonucleotide has a fluorescent label; and a third nucleotide which includes some or all the primer sequence contained in the first oligonucleotide but is not labeled, the first and third oligonucleotide being combined in a molar ratio of 2.8 to 8.2.Type: ApplicationFiled: March 15, 2013Publication date: December 5, 2013Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Nathan Tanner, Yinhua Zhang, Thomas C. Evans
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Patent number: 7700758Abstract: A method for obtaining a mixture of heterogenous short double-stranded RNA molecules suitable for use in gene silencing (hsiRNA) by subjecting large double-stranded RNA to enzymatic cleavage under specified conditions. The resulting mixture consistently includes enhanced representation of fragments having a size of 21-22 nucleotides absent any fractionation step. The fragments contain sequences that collectively span the entire length of the large double-stranded RNA from which they are derived. Double-stranded RNA with sequences that individually represent segments of a target mRNA may be analyzed using the methods described herein to identify the most active subset of hsiRNA fragments or individual siRNA fragments for achieving gene silencing for any gene or transcribed sequences. A method is additionally provided for preparing and cloning DNA encoding selected siRNA, hsiRNA mixtures or hairpin sequences to provide a continuous supply of a gene silencing reagent derived from any long double-stranded RNA.Type: GrantFiled: July 18, 2003Date of Patent: April 20, 2010Assignee: New England Biolabs, Inc.Inventors: George Tzertzinis, George Feehery, Christopher Noren, Corinna Tuckey, Larry McReynolds, Yinhua Zhang
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Publication number: 20080206835Abstract: A method for obtaining a mixture of heterogenous short double-stranded RNA molecules suitable for use in gene silencing (hsiRNA) by subjecting large double-stranded RNA to enzymatic cleavage under specified conditions. The resulting mixture consistently includes enhanced representation of fragments having a size of 21-22 nucleotides absent any fractionation step. The fragments contain sequences that collectively span the entire length of the large double-stranded RNA from which they are derived. Double-stranded RNA with sequences that individually represent segments of a target mRNA may be analyzed using the methods described herein to identify the most active subset of hsiRNA fragments or individual siRNA fragments for achieving gene silencing for any gene or transcribed sequences. A method is additionally provided for preparing and cloning DNA encoding selected siRNA, hsiRNA mixtures or hairpin sequences to provide a continuous supply of a gene silencing reagent derived from any long double-stranded RNA.Type: ApplicationFiled: February 11, 2008Publication date: August 28, 2008Applicant: New England Biolabs, Inc.Inventors: George Tzertzinis, George Feehery, Corinna Tuckey, Christopher Noren, Larry McReynolds, Yinhua Zhang
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Publication number: 20070099234Abstract: Methods and kits are provided for detecting chitin in biological samples using chitin-binding domain (CBD).Type: ApplicationFiled: June 7, 2004Publication date: May 3, 2007Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Yinhua Zhang, Larry McReynolds
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Publication number: 20060111848Abstract: Present embodiments of the invention describe computational methods for performing a systematic, genome-wide search for novel drug targets in pathogenic organisms for example, the human filarial parasites. Cofactor independent phosphoglycerate mutase (iPGM) was identified by this search as a candidate target for identifying therapeutic agents for use in treating animal or plant subjects infected with parasitic nematodes, microbial pathogens including microsporidia, fungi etc. A consensus amino acid or nucleotide sequence that characterizes iPGM is further provided.Type: ApplicationFiled: December 22, 2005Publication date: May 25, 2006Applicant: New England Biolabs, Inc.Inventors: Clotilde Carlow, Yinhua Zhang, Jeremy Foster, Sanjay Kumar