Abstract: The present disclosure provides a nucleic acid sequence for regulating the transcription of a nucleic acid fragment of interest, wherein the nucleic acid sequence comprises at least 2 copies of TetO-operator sequences capable of binding to a transactivator rtTA regulatable by tetracycline or a derivative thereof, and 1 copy of minimal promoter sequence containing a TATA box sequence, and at least 1 copy of a CuO-operator sequence capable of binding to a transcription repressor CymR regulatable by cumate. The present disclosure also provides a vector and a host cell containing the nucleic acid sequence, and a method for inducing the expression of a nucleic acid fragment of interest in a host cell.

Abstract: The application provides a battery management method and a power supply system. The battery management method includes: providing the power supply system comprising a power supply device and a plurality of battery systems connected in parallel, the power supply device electrically configured to control the charging or discharging of each of the plurality of battery systems connected to each of the plurality of battery systems; detecting an actual voltage of each battery system; controlling the start of battery systems with a low actual voltage for charging or discharging; and controlling the start of battery systems with a high actual voltage after the actual voltage of each battery system is balanced. The battery systems are directly controlled by the power supply device when connected in parallel to the power supply system, such that unlimited capacity expansion may be realized without redesigning the battery systems or adding a BMS.

Abstract: The present disclosure relates to a method for constructing a producer cell and the producer cell obtained by the method, wherein the producer cell is for producing a retroviral vector carrying a nucleic acid fragment of interest.

Abstract: The present disclosure provides a nucleic acid sequence for regulating the transcription of a nucleic acid fragment of interest, wherein the nucleic acid sequence comprises at least 2 copies of TetO-operator sequences capable of binding to a transactivator rtTA regulatable by tetracycline or a derivative thereof, and 1 copy of minimal promoter sequence containing a TATA box sequence, and at least 1 copy of a CuO-operator sequence capable of binding to a transcription repressor CymR regulatable by cumate. The present disclosure also provides a vector and a host cell containing the nucleic acid sequence, and a method for inducing the expression of a nucleic acid fragment of interest in a host cell.