Abstract: A scalable method for producing DNA/polycation particles having an optimal, defined particle size with multiple virus assembly plasmids for efficient transfection of viral production cells in suspension cultures. The presently disclosed DNA/polycation particles yield superior and reproducible transfection activity and shelf stability in the suspension form and can be used as an off-the-shelf product. The presently disclosed DNA/polycation particle formulation can potentially simplify and streamline the viral manufacturing process and improve production quality and consistency.

Abstract: Methods for preparing nucleic acid/lipid particles of an optimum particle size for efficient transfection of cells in vitro and in vivo local transfection are provided. The method is based on kinetic control of the nucleic acid/lipid nanoparticle assembly to prepare shelf-stable particles with defined sizes between about 50 nm and 1200 nm. The size-dependent characteristics of the nucleic acid/lipid particle-mediated transfection for the size range between 50 nm and 1200 nm also is provided.

Abstract: Disclosed are the optimal composition and size of DNA/polycation particles for efficient transfection of viral production cells in both adherent and suspension cultures. The size-dependent feature of DNA/polycation particle-mediated transfection for particles between 50 nm and 1000 nm also is disclosed. A new scalable method based on kinetic control of DNA/polycation nanoparticle assembly to prepare shelf-stable particles with defined sizes between 50 nm and 1000 nm also is disclosed. The presently disclosed DNA/polycation particles yield superior and reproducible transfection activity and shelf stability and can be used as an off-the-shelf product.

Abstract: A system and method for a multi-party computation (MPC) is provided. In implementations, a method includes identifying a blockchain network and a computing device to perform an MPC based on an index; generating an MPC request including a function to be performed by the blockchain network and the computing device, data required for the function, and a verification policy defining a verification protocol to be performed by the blockchain network and the computing device; sending the MPC request to the blockchain network and the computing device; and receiving responses from a representative computing node of the blockchain network and the computing device, wherein each of the responses includes: an output of an MPC protocol performed by the blockchain network and the computing device to jointly compute the function while keeping the inputs private from one another and private from the computing system; and a proof based on the verification policy.

Abstract: The invention provides a dentition movement track indicator and a tracing method. The device includes an intra-oral fixation assembly and an extra-oral movement indicating assembly, wherein the intra-oral fixation assembly includes a lower denture base, a left side retention steel wire, a right side retention steel wire and a positioning steel wire. The lower denture base is arranged on the lingual side of the lower dentition and respectively extends from a lower incisor to the far center of the last tooth on the left side and the right side and clings to the lingual mucous membrane. The left side retention steel wire and the right side retention steel wire are distributed in a left-right symmetry mode. The extra-oral movement indicating assembly includes a connecting rod, an arc rod, an indicating plate and a plurality of rectangular parallelepiped thin rods. According to the dentition movement track indicator, an intra-oral fixation assembly.

Abstract: The invention provides a dentition movement track indicator and a tracing method. The device includes an intra-oral fixation assembly and an extra-oral movement indicating assembly, wherein the intra-oral fixation assembly includes a lower denture base, a left side retention steel wire, a right side retention steel wire and a positioning steel wire. The lower denture base is arranged on the lingual side of the lower dentition and respectively extends from a lower incisor to the far center of the last tooth on the left side and the right side and clings to the lingual mucous membrane. The left side retention steel wire and the right side retention steel wire are distributed in a left-right symmetry mode. The extra-oral movement indicating assembly includes a connecting rod, an arc rod, an indicating plate and a plurality of rectangular parallelepiped thin rods. According to the dentition movement track indicator, an intra-oral fixation assembly.

Abstract: A method and system for manufacturing a perpendicular magnetic recording transducer by a process that includes plating is described. The method and system include forming a chemical mechanical planarization (CMP) uniformity structure around a perpendicular magnetic recording pole. The CMP uniformity structure has a height substantially equal to a desired pole height. The method and system also include fabricating an insulator on the CMP uniformity structure and performing a CMP to remove a portion of the insulator. The CMP exposes a portion of the perpendicular magnetic recording pole and planarizes an exposed surface of the perpendicular magnetic recording transducer.

Abstract: Methods are provided for fabricating an electrical lapping guide, and for using the electrical lapping guide to fabricate a transducer of a magnetic recording head. Additionally, transducers comprising an electrical lapping guide are also provided. Methods of fabricating the electrical lapping guide comprise forming a resistive element proximate to a shield pedestal. Accordingly, the resistive element is both aligned with the shield pedestal in a transverse direction, and closely spaced from the shield pedestal in a longitudinal direction. The resistance of the resistive element correlates well with a throat height of the shield pedestal, and the correlation is utilized in the methods of fabricating the transducer. When a magnetoresistive stripe is uses as a second electrical lapping guide while fabricating the transducer, the two can be used to control a tilt angle in the transverse direction during lapping.

Abstract: A head including a write element for writing data to a magnetic media, and methods for its production are provided. A write element of the invention includes one or more of a recessed first pole, a heat sink layer, and a shortened yoke length. A method of the invention provides forming an anti-reflective layer before forming a mask layer. During photolithography the anti-reflective layer suppresses undesirable reflections off of features, such as vertical sidewalls, that otherwise limit how closely to such features portions of the mask layer can be formed.

Abstract: A method to fabricate a tunneling magnetoresistive (TMR) read transducer is disclosed. An insulative layer is deposited on a wafer substrate, and a bottom lead is deposited over the insulative layer. A laminated TMR layer, having a plurality of laminates, is deposited over the bottom lead. A TMR sensor having a stripe height is defined in the TMR layer, and a parallel resistor and first and second shunt resistors are also defined in the TMR layer. A top lead is deposited over the TMR sensor. The parallel resistor is electrically connected to the bottom lead and to the top lead. The first shunt resistor is electrically connected to the bottom lead and the wafer substrate, and the second shunt resistor is electrically connected to the top lead and the wafer substrate.

Abstract: A method for fabricating a magnetic recording head writer. The writer includes a bottom magnetic pole and a write gap formed over the bottom pole and a coil trench formed in the bottom pole. A top magnetic pole is provided as two layers with the first layer including front and back tips with spaced apart walls positioned adjacent the trench bottom defining trench sides. A pole cover layer is included that is made up of a thin layer of insulating material deposited to cover sides and bottom of the coil trench. A bottom coil is formed on the pole cover layer in the bottom of the coil trench and coil insulation is provided between coil elements and adjacent trench walls and covemg the coil. A top coil with insulation is formed over the planarized bottom coil insulation and the top pole second layer is formed over the top coil.

Abstract: A head including a write element for writing data to a magnetic media, and methods for its production are provided. A write element of the invention includes one or more of a recessed first pole, a heat sink layer, and a shortened yoke length. A method of the invention provides forming an anti-reflective layer before forming a mask layer. During photolithography the anti-reflective layer suppresses undesirable reflections off of features, such as vertical sidewalls, that otherwise limit how closely to such features portions of the mask layer can be formed.

Abstract: A head including a write element for writing data to a magnetic media, and methods for its production are provided. A write element of the invention includes one or more of a recessed first pole, a heat sink layer, and a shortened yoke length. A method of the invention provides forming an anti-reflective layer before forming a mask layer. During photolithography the anti-reflective layer suppresses undesirable reflections off of features, such as vertical sidewalls, that otherwise limit how closely to such features portions of the mask layer can be formed.

Abstract: A writer for magnetic recording heads. The writer includes a bottom magnetic pole and a write gap formed over the bottom pole and a coil trench formed in the bottom pole. A top magnetic pole is provided as two layers with the first layer including front and back tips with spaced apart walls positioned adjacent the trench bottom defining trench sides. A pole cover layer is included that is made up of a thin layer of insulating material deposited to cover the sides and bottom of the coil trench. A bottom coil is formed on the pole cover layer in the bottom of the coil trench and coil insulation is provided between the coil elements and adjacent the trench walls and covering the coil. A top coil with insulation is formed over the planarized bottom coil insulation and the top pole second layer is formed over the top coil.

Abstract: A writer for magnetic recording heads. The writer includes a bottom magnetic pole and a write gap formed over the bottom pole and a coil trench formed in the bottom pole. A top magnetic pole is provided as two layers with the first layer including front and back tips with spaced apart walls positioned adjacent the trench bottom defining trench sides. A pole cover layer is included that is made up of a thin layer of insulating material deposited to cover the sides and bottom of the coil trench. A bottom coil is formed on the pole cover layer in the bottom of the coil trench and coil insulation is provided between the coil elements and adjacent the trench walls and coverng the coil. A top coil with insulation is formed over the planarized bottom coil insulation and the top pole second layer is formed over the top coil.

Abstract: A method for forming a plated layer. There is first provided a substrate. There is then formed over the substrate a masking frame employed for masking frame plating a masking frame plated layer within the masking frame, where the masking frame is fabricated to provide an overhang of an upper portion of the masking frame spaced further from the substrate with respect to a lower portion of the masking frame spaced closer to the substrate. Finally, there is then plated the masking frame plated layer within the masking frame. The method is useful for forming masking frame plated magnetic pole tip stack layers with enhanced planarity dimensional control within magnetic transducer elements.

Abstract: A method for forming a plated layer. There is first provided a substrate. There is then formed over the substrate a masking frame employed for masking frame plating a masking frame plated layer within the masking frame, where the masking frame is fabricated to provide an overhang of an upper portion of the masking frame spaced further from the substrate with respect to a lower portion of the masking frame spaced closer to the substrate. Finally, there is then plated the masking frame plated layer within the masking frame. The method is useful for forming masking frame plated magnetic pole tip stack layers with enhanced planarity dimensional control within magnetic transducer elements.

Abstract: A method for forming a plated layer. There is first provided a substrate. There is then formed over the substrate a masking frame employed for masking frame plating a masking frame plated layer within the masking frame, where the masking frame is fabricated to provide an overhang of an upper portion of the masking frame spaced further from the substrate with respect to a lower portion of the masking frame spaced closer to the substrate. Finally, there is then plated the masking frame plated layer within the masking frame. The method is useful for forming masking frame plated magnetic pole tip stack layers with enhanced planarity dimensional control within magnetic transducer elements.