Abstract: This disclosure provides a method for stabilizing both lipase and protease in liquid enzymatic laundry detergent, comprising steps of: first, self-assembling conjugated linoleic acid with protease and lipase, respectively, to form vesicles encapsulated protease and vesicles encapsulated lipase at a low Ca2+ concentration; then, mixing the solution of vesicles encapsulated protease and the solution of vesicles encapsulated lipase, and then partially cross-linking conjugated linoleic acid molecules on the vesicles' surface. The obtained enzyme vesicles, after being concentrated, can be used directly in liquid laundry detergent. The lipase in the liquid laundry detergent will not be degraded by protease, and the enzymes in vesicles are able to resist against surfactant inhibition. Thus, the enzyme activities can be maintained in the liquid laundry detergent, and the enzymatic vesicles will be broken to release enzymes, when the liquid laundry detergent is used at a higher Ca2+ concentration such as in tap water.

Abstract: This disclosure provides a method for stabilizing both lipase and protease in liquid enzymatic laundry detergent, comprising steps of: first, self-assembling conjugated linoleic acid with protease and lipase, respectively, to form vesicles encapsulated protease and vesicles encapsulated lipase at a low Ca2+ concentration; then, mixing the solution of vesicles encapsulated protease and the solution of vesicles encapsulated lipase, and then partially cross-linking conjugated linoleic acid molecules on the vesicles' surface. The obtained enzyme vesicles, after being concentrated, can be used directly in liquid laundry detergent. The lipase in the liquid laundry detergent will not be degraded by protease, and the enzymes in vesicles are able to resist against surfactant inhibition. Thus, the enzyme activities can be maintained in the liquid laundry detergent, and the enzymatic vesicles will be broken to release enzymes, when the liquid laundry detergent is used at a higher Ca2+ concentration such as in tap water.