Abstract: Disclosed is a method for non-rigid image registration, comprising: dividing a subject image and a target image into a plurality of subject sub-images and a plurality of target sub-images, respectively; registering, in parallel, each of the subject sub-images and the corresponding one of the target sub-images to obtain a transformation matrix of the each of the subject sub-images relative to the corresponding one of the target sub-images; transforming, based on by the obtained transformation matrices, the subject sub-images into images corresponding to the target sub-images; and merging the transformed images to form a final registered image having the resolution of the target image. A system and a device for non-rigid image registration are also enclosed within the disclosure.

Abstract: The present invention relates to a method for labeling a membrane-localized protein by introducing a biotin target sequence tag into at least one loop domain of a membrane-localized protein. The method of the invention is useful for labeling an ion channel protein such as CFTR or mutants thereof. A method for identifying an agent which corrects protein misfolding of a membrane-localized protein is also provided.