Abstract: The present invention provides a disposable 3-D structured depended sequential lateral flow capillary device comprising: a housing; a 3-D structured capillary flow matrix; and (iii) an absorption portion, and use thereof in a method for determining the presence of an analyte in a sample.

Abstract: A tank apparatus (10) for horizontal electrophoresis that comprises a base member (12) having at least first and second independent receptacles (16, 18) for accommodating buffer solution. Each of the receptacles comprises an inlet (28, 28?) for allowing communication with the external environment. A cover member (14) covers at least the first and second receptacles, thereby forming first and second buffer chambers. The cover member comprising suitable openings (40, 42) for allowing a portion of an electrophoresis gel cassette to be inserted therethrough, such that the electrophoresis gel is in communication with the contents of each of the first and second receptacles. Each of a pair of electrodes (33, 33?) is situated in one of each of the first and second receptacles, wherein the electrodes are connectable to an electrical power supply.

Abstract: Disclosed is a precast polyacrylamide gel for use in gel electrophoresis, comprising polyacrylamide and an aqueous Tris 0.04 M to 0.15 M solution, at least one first ampholyte exhibiting an isoelectric point (pI) of from 5.4 to 6.4 at a total concentration of from 0.01 M to 0.4 M; and at least one second ampholyte exhibiting an isoelectric point (pI) of from 2.5 to 3.5 to adjust the pH to between 5.5 and 7.5 at the temperature of 25° C. The precast gels can be used for electrophoresis of oligopeptides, polypeptides, oligonucleotides, and polynucleotides under denaturating or nondenaturing conditions, and exhibit long shelf-life.

Abstract: Provided is a precast polyacrylamide gel for use in gel electrophoresis, having a shelf-life of at least 12 months. The gel comprises a sulfonated tertiary or secondary amine having a pKa of 6.6-8.0, ampholytes exhibiting pI between 5.4 to 6.4, Tris, and hydrochloric acid to adjust the pH to between 5.5 and 7.5.

Abstract: A tank apparatus (10) for horizontal electrophoresis that comprises a base member (12) having at least first and second independent receptacles (16, 18) for accommodating buffer solution. Each of the receptacles comprises an inlet (28, 28?) for allowing communication with the external environment. A cover member (14) covers at least the first and second receptacles, thereby forming first and second buffer chambers. The cover member comprising suitable openings (40, 42) for allowing a portion of an electrophoresis gel cassette to be inserted therethrough, such that the electrophoresis gel is in communication with the contents of each of the first and second receptacles. Each of a pair of electrodes (33, 33?) is situated in one of each of the first and second receptacles, wherein the electrodes are connectable to an electrical power supply.

Abstract: The present invention is directed to a solidified hybrid gel for use in an electrophoresis process, having a solidified first gel portion juxtaposed with a solidified second gel portion. The first gel portion is capable of accommodating therein one or more samples for electrophoresis after said first gel portion is in solidified form, and the second gel portion is adapted for enabling an electrophoresis process to be applied to such a sample that may be accommodated in said first gel portion. Thus, the hybrid gel may be provided in a precast form to users, ready for use. The invention is also directed to methods for providing such a gel, apparatuses for accommodating such a gel, and methods for carrying out electrophoresis on a sample comprised in such a gel.

Abstract: A method for orientation-directed construction of a construct including at least two nucleic acid segments of interest. Products are provided having phosphorylated blunt ends derived from the nucleic acid segments of interest. Separate ligation reactions are performed wherein in each reaction the phosphorylated blunt-ended products of two different segments obtained in the previous step are ligated to create a combined ligated sequence. PCR amplification reaction is performed using the combined ligated sequence obtained in the previous step as a template and specific enrichment primers direct the PCR amplification towards the desired orientation. Each combined ligated sequence is amplified in a separate reaction creating a combined product having phosphorylated blunt ends. The combined product is isolated and purified to have the enriched desired orientation.

Abstract: An apparatus for electrophoresis having a first gel matrix, adapted for performing an electrophoretic process therein, in communication with a second gel matrix, both being accommodated within a suitable housing. The housing has a first opening adapted to permit ionic communication between the first gel matrix and an external ionic buffer solution, and a second opening adapted to permit ionic communication between the second gel matrix and an external ionic buffer solution. The second gel has at least one suitable absorption material capable of retaining therein at least one target substance capable of migrating thereto from the first gel matrix when an electrophoretic process is performed in the first matrix.

Abstract: The present invention is directed to a solidified hybrid gel for use in an electrophoresis process, having a solidified first gel portion juxtaposed with a solidified second gel portion. The first gel portion is capable of accommodating therein one or more samples for electrophoresis after said first gel portion is in solidified form, and the second gel portion is adapted for enabling an electrophoresis process to be applied to such a sample that may be accommodated in said first gel portion. Thus, the hybrid gel may be provided in a precast form to users, ready for use. The invention is also directed to methods for providing such a gel, apparatuses for accommodating such a gel, and methods for carrying out electrophoresis on a sample comprised in such a gel.

Abstract: A device having a processing chamber, for electroelution and/or dialysis of a substance carried in a sample with respect to an external liquid medium. The chamber has a closed end and an opening at the other end, sufficiently large to permit a sample, particularly a gel contained sample, to be inserted thereto. The chamber has two portals, each laterally disposed with respect to the opening. A tubular membrane covers the portals, and is sealingly fixed onto an outside surface of the housing. The device provides high yield recovery, saves time and allows for easy handling, especially regarding loading an unloading of small volume samples for dialysis, or inserting the gel slice containing the macromolecule sample. The device may be partially immersed in the liquid medium, with the opening above the liquid surface. Optionally, the device may be hermetically sealed via a cap, and fully immersed in the liquid medium.

Abstract: A cis-acting nucleotide sequence which is capable of rendering the removal of introns from a precursor transcript encoded by a gene, which gene harbors at least one such cis-acting nucleotide sequence, occurring during the production of mRNA of a gene, dependent upon activation of a trans-acting factor. The trans-acting factor is an RNA-activated protein kinase which is capable of phosphorylating the ?-subunit of eukaryotic initiation factor 2. The trans-acting factor is preferably, the RNA-activated protein kinase (PKR). The cis-acting nucleotide sequence can be derived from the 3? untranslated region of the human tumor necrosis factor ? gene (TNF-?3?-UTR) and may comprise the nucleotide sequence as denoted by SEQ ID NO:1 or biologically functional fragments, derivatives, mutants and homologues thereof.

Abstract: A method for orientation-directed construction of a construct including at least two nucleic acid segments of interest. Products are provided having phosphorylated blunt ends derived from the nucleic acid segments of interest. Separate ligation reactions are performed wherein in each reaction the phosphorylated blunt-ended products of two different segments obtained in the previous step are ligated to create a combined ligated sequence. PCR amplification reaction is performed using the combined ligated sequence obtained in the previous step as a template and specific enrichment primers direct the PCR amplification towards the desired orientation. Each combined ligated sequence is amplified in a separate reaction creating a combined product having phosphorylated blunt ends. The combined product is isolated and purified to have the enriched desired orientation.

Abstract: An apparatus for electrophoresis having a first gel matrix, adapted for performing an electrophoretic process therein, in communication with a second gel matrix, both being accommodated within a suitable housing. The housing has a first opening adapted to permit ionic communication between the first gel matrix and an external ionic buffer solution, and a second opening adapted to permit ionic communication between the second gel matrix and an external ionic buffer solution. The second gel has at least one suitable absorption material capable of retaining therein at least one target substance capable of migrating thereto from the first gel matrix when an electrophoretic process is performed in the first matrix.

Abstract: A solidified hybrid gel for use in an electrophoresis process that has a solidified first gel portion juxtaposed with a solidified second gel portion. The first gel portion is capable of accommodating therein one or more samples for electrophoresis after said first gel portion is in solidified form, and the second gel portion is adapted for enabling an electrophoresis process to be applied to such a sample that may be accommodated in said first gel portion. Thus, the hybrid gel may be provided in a precast form to users, ready for use. The invention is also directed to methods for providing such a gel, apparatuses for accommodating such a gel, and methods for carrying out electrophoresis on a sample comprised in such a gel.

Abstract: A device having a processing chamber, particularly for electroelution and/or dialysis of a substance carried in a sample with respect to an external liquid medium. The chamber is closed at one end and has an opening al the other longitudinal end sufficiently large to permit a sample, and in particular a gel contained sample, to be inserted thereinto. The chamber has a pair of portals laterally disposed with respect to the opening. The portals are covered with a typically tubular membrane, which is sealingly fixed onto an outside surface of the housing defining the chamber, typically by means of a tubular sleeve having portals that align with the portals of the chamber, via an annular sealing arrangement. The device provides high yield recovery, saves time, and allows for relatively easy handling specially regarding loading and unloading of small volume of samples to be dialyzed or inserting the slice of gel containing the macromolecule sample.

Abstract: A cis-acting nucleotide sequence which is capable of rendering the removal of introit/s from a precursor transcript encoded by a gene, which gene harbors at least one such cis-acting nucleotide sequence, occurring during the production of mRNA of said gene, dependent upon activation of a trans-acting factor. The trans-acting factor is an RNA-activated protein kinase which is capable of phosphorylated the &agr;-subunit of eucaryotic initiation factor 2, or the RNA-activated protein kinase (PAR). The cis-acting nucleotide sequence can be derived from the 3′ untranslated region of the human tumor necrosis factor &agr; gene (TNF-&agr; 3′-UTR).