Abstract: Disclosed herein are compounds, compositions, and methods for identifying substrates of E3 ubiquitin ligases. The proteins, vectors, compositions, and may be utilized in methods for orthogonal ubiquitin transfer (OUT) between variant components of the ubiquitin cascade such as a variant ubiquitin (UB), a variant ubiquitin activating enzyme (E1), a variant ubiquitin-conjugating enzyme (E2), and a variant ubiquitin ligase (E3), which may include a variant HECT type, U-box type, RBR type, and/or Ring type E3 ligase. The variant components of the ubiquitin cascade typically include one or more mutations relative to their wild-type counterparts, such as amino acid substitutions, such that the variant components interact specifically and orthogonally with each of their variant components and do not interact with the wild-type counterpart of their variant components.

Abstract: Methods and systems for detecting alignment of a substrate during a printing process are described. In an embodiment, a printing apparatus may comprise an apparatus polarization area. A substrate may be configured with a substrate polarization area. The substrate may be arranged on the printing apparatus during printing such that the substrate polarization area overlaps the apparatus polarization area to form an alignment area. During printing, light may be radiated onto the alignment area to generate polarized light. The polarized light may be received by a polarized light receiving device. Characteristics of the light received by the polarized light receiving device may be monitored for variations. The variations may indicate that the substrate is not properly aligned for printing on the printing apparatus.

Abstract: Technologies are provided for filtering user's actions based on user's mood. User's actions may include online actions. To prevent “in the heat of the moment” actions, which a user may regret later, a mood based filter may identify a user action such as posting a message to a social network, a professional network, an email network, a blog, or an instant message network. An automatic system action may then be taken based on the detected user's mood. In some examples, the user's mood may be assigned to a numeric or alphanumeric category. Various system actions such as blocking or delaying the user action may be tied or associated to the user's mood category through one or more predefined, customizable rules. In other examples, a user confirmation of the user action may also be included in addition to the automatic system action.

Abstract: Printing apparatuses for detection of substrate alignment, methods for manufacture and use thereof, and substrates are disclosed. One printing apparatus may include a printing assembly having at least one conductive alignment sensor with a plurality of inductive coils configured to generate an electromagnetic field. The printing assembly may receive at least one substrate having at least one conductive portion and align the substrate such that the plurality of inductive coils and the conductive portion substantially overlap with each other to form an electromagnetic field having a direction from the at least one conductive alignment sensor to the at least one conductive portion. The direction of the electromagnetic field with respect to the conductive alignment sensor may form an angle. The conductive alignment sensor may be configured to detect a movement of the conductive portion when a change to the angle causes a current change through the plurality of inductive coils.

Abstract: Methods and systems for detecting alignment of a substrate during a printing process are described. In an embodiment, a printing apparatus may comprise an apparatus polarization area. A substrate may be configured with a substrate polarization area. The substrate may be arranged on the printing apparatus during printing such that the substrate polarization area overlaps the apparatus polarization area to form an alignment area. During printing, light may be radiated onto the alignment area to generate polarized light. The polarized light may be received by a polarized light receiving device. Characteristics of the light received by the polarized light receiving device may be monitored for variations. The variations may indicate that the substrate is not properly aligned for printing on the printing apparatus.

Abstract: Technologies are provided for filtering user's actions based on user's mood. User's actions may include online actions. To prevent “in the heat of the moment” actions, which a user may regret later, a mood based filter may identify a user action such as posting a message to a social network, a professional network, an email network, a blog, or an instant message network. An automatic system action may then be taken based on the detected user's mood. In some examples, the user's mood may be assigned to a numeric or alphanumeric category. Various system actions such as blocking or delaying the user action may be tied or associated to the user's mood category through one or more predefined, customizable rules. In other examples, a user confirmation of the user action may also be included in addition to the automatic system action.