Abstract: In various aspects and embodiments, the present disclosure provides methods for making glycosylated products, as well as bacterial cells and uridine diphosphate (UDP)-dependent glycosyltransferase (UGT) enzymes useful for the same. In other aspects and embodiments, the disclosure provides methods for high yield and/or high purity recovery of such glycoside products from microbial cultures or cell free reactions. In various aspects and embodiments, the disclosure provides for whole cell bioconversion processes involving the glycosylation of a desired substrate, and/or the recovery of the glycosylated product at high yield and/or high purity.

Abstract: The present disclosure provides methods and compositions for producing artemisinic acid, dihydroartemisinic acid or artemisinin. In various aspects, the present disclosure provides enzymes, polynucleotides encoding said enzymes, and recombinant microbial host cells (or microbial host strains) for the production of artemisinic acid, dihydroartemisinic acid or artemisinin. The present disclosure further provides methods of making pharmaceutical products containing artemisinic acid, dihydroartemisinic acid or artemisinin.