Abstract: The present invention relates to an indicator molecule for the toxigenicity of aflatoxigenic fungi and the use thereof. The amino acid sequence of the indicator molecule ABFT-YJFZ01 for the toxigenicity of the aflatoxigenic fungi is as represented in SEQ ID NO. 1. The indicator molecule is used for identifying the aflatoxin-producing capacity of the Aspergillus aflatoxigenic fungi and identifying whether the aflatoxigenic fungi with high toxigenicity is present in farmland, agricultural products and feed, and is easy to popularize and apply.

Abstract: The present disclosure relates to a microbial agent with functions of preventing and controlling aflatoxin and aflatoxigenic strain thereof and promoting yield increase of crops. The microbial agent is compounded from 5 microbes comprising Bacillus amyloliquefaciens, Brevibacillus laterosporu, Bacillus mucilaginosus Krassilnikov, Enterobacter ludwigii and Myroides odoratimimus by separate fermentative cultivation, concentration and mixing. The microbial agent is applied to a field planting stage of crops such as peanuts, which can effectively reduce the abundance and infection probability of toxin-producing strain such as Aspergillus flavus in soil from the source, reduce the risk of aflatoxin pollution of peanuts after production, improve the quality and safety level of peanuts, and at the same time, promote crop growth, enhance the resistance, and improve the full pod rate and yield, and has significant economic, social and ecological benefits.

Abstract: The present disclosure belongs to the field of microbial technology, and in particular relates to a microbial agent for promoting root nodule number increase and root nodule nitrogenase activity increase in leguminous crops and use thereof. The microbial agent is compounded from 4 microbes including Bacillus amyloliquefaciens, Brevibacillus laterosporu, Bacillus mucilaginosus Krassilnikov, and Enterobacter ludwigii by separate fermentative cultivation, concentration and mixing. The microbial agent can effectively promote root nodule number increase and root nodule nitrogenase activity increase, promote crop growth, and improve crop yield and product quality.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.

Abstract: An immunoadsorbent and a composite affinity column for purifying fumonisin B1, anguidin, T-2 toxin, zearalenone, and vomitoxin. The immunoadsorbent includes a solid phase carrier, and a fumonisin B1 monoclonal antibody, an anguidin monoclonal antibody, a T-2 toxin monoclonal antibody, a zearalenone monoclonal antibody and a vomitoxin monoclonal antibody which are coupled to the solid phase carrier, the anguidin monoclonal antibody is a monoclonal antibody secreted by a hybridoma cell strain DAS5G11E7 having an accession number of CCTCCNO:C201881. The affinity column can be used for high performance liquid chromatography-mass spectrometry detection of the fumonisin B1, the anguidin, the T-2 toxin, the zearalenone and the vomitoxin, and has stable performance. Furthermore, an economical, quick, precise and safe detection method is established of the basis of the affinity column, and can be used for purifying and detecting samples of the five toxins without mutual interference and influence.

Abstract: A time-resolved fluorescence kit for synchronously detecting 4,15-diacetoxyscirpenol, deoxynivalenol and T-2 toxin. The kit includes an immunochromatography time-resolved fluorescence test strip and a sample reaction bottle containing freeze-dried products of europium-labeled monoclonal antibodies of toxins, where the immunochromatography time-resolved fluorescence test strip includes a liner, where a water absorption pad, a detection pad and a sample pad are sequentially attached to one side of the liner from top to bottom, adjacent pads are connected in an overlapping manner at a joint, the detection pad uses a nitrocellulose membrane as a base pad, a transverse quality control line and detection lines are arranged on the nitrocellulose membrane from top to bottom, the quality control line is coated with a rabbit antimouse polyclonal antibody, the three detection lines are located below the quality control line, and the detection lines each are coated with a toxin-protein conjugate.

Abstract: The present invention relates to an aflatoxin contamination risk warning molecule and use thereof. The steps are as follows: weighing a quantitative sample, extracting the aflatoxin contamination risk warning molecule to obtain a sample extract, and detecting and analyzing the sample extract to obtain a quantitative result of the aflatoxin contamination risk warning molecule; performing modeling with a chemometrics method using the content of one or more of the aflatoxin contamination risk warning molecules as a variable to obtain a classification prediction model, and performing risk assessment on aflatoxin contamination risk of the sample based on the classification prediction model, wherein a warning molecule of an aflatoxin toxigenic strain is one or a combination of more than one of versiconol (VOH), versicolorin B (Ver B), and 5-methoxysterigmatocystin (5-MST).

Abstract: A method for identifying and evaluating toxigenic capability of an aflatoxigenic strain. A ratio of the aflatoxin yield to Nor-1 gene transcriptional quantity is determined to have high relative stability. An Aspergillus flavus strain toxigenic capability identification model is established, and thus a regression equation between the Aspergillus flavus toxigenic capability and the ratio AFT/Nor-1 of the aflatoxin yield to the Nor-1 gene transcriptional quantity is obtained.

Abstract: The present invention relates to the microbiological field, and particularly relates to a Leclercia adcarboxglata biocontrol strain efficiently inhibiting production of aflatoxins by Aspergillus flavus. The Leclercia adcarboxglata biocontrol strain Wt16 was deposited at China Center for Type Culture Collection (CCTCC) on Jun. 13, 2017, and the accession number of the strain is CCTCC No. M2017331. The Leclercia adcarboxglata strain Wt16 is isolated from peanut pods for the first time which can significantly inhibit aflatoxins production by Aspergillus flavus, and also has an extremely good effect on inhibiting aflatoxins production by Aspergillus flavus for peanuts from different sources.

Abstract: The present invention relates to the microbiological field, and particularly relates to a Serratia marcescens biocontrol strain efficiently inhibiting production of aflatoxins by Aspergillus flavus and an application thereof. The Serratia marcescens biocontrol strain 3J4SM was deposited at China Center for Type Culture Collection (CCTCC) on Jun. 13, 2017, and the accession number of the strain is CCTCC No. M2017328. The Serratia marcescens biocontrol strain 3J4SM can be used to inhibit production of aflatoxins by Aspergillus flavus and prevent grain crops from aflatoxin pollution.

Abstract: The present invention relates to the microbiological field, and particularly relates to a leclercia adcarboxglata biocontrol strain efficiently inhibiting production of aflatoxins by Aspergillus flavus. The leclercia adcarboxglata biocontrol strain Wt16 was deposited at China Center for Type Culture Collection (CCTCC) on Jun. 13, 2017, and the accession number of the strain is CCTCC No. M2017331. The leclercia adcarboxglata strain Wt16 is isolated from peanut pods for the first time which can significantly inhibit aflatoxins production by Aspergillus flavus, and also has an extremely good effect on inhibiting aflatoxins production by aspergillus flavus for peanuts from different sources.