Patents by Inventor Yoichi Fujiyama

Yoichi Fujiyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230313103
    Abstract: A cell culture container includes: a container body; a cell culture insert having a tubular portion and an oxygen-permeable membrane; and a lid member. The container body is provided with an opening that communicates with an interior of the container body. The tubular portion includes an upper end and a lower end, and is inserted in the opening such that the lower end is located inside the container body. The lower end side of the tubular portion is closed by the membrane. The upper end side of the tubular portion is closed by the lid member. The lid member includes a lower surface that faces toward an interior of the tubular portion. The lid member is provided with an electrode insertion port and a culture medium outlet port that each communicate with the interior of the tubular portion at the lower surface.
    Type: Application
    Filed: July 7, 2021
    Publication date: October 5, 2023
    Inventors: Tsunehiro INOUE, Toyoyuki HASHIMOTO, Yasuko YONEDA, Kenji TAKUBO, Yoichi FUJIYAMA, Tomoki OHKUBO, Eiichi OZEKI, Ryogo TAKAI, Hiroomi GOTO, Sadamu TOMITA
  • Patent number: 11549092
    Abstract: A cell evaluation device includes: a porous membrane having a first main face and a second main face; a first passage having a first passage portion facing a first area on which cells are placed in the first main face of the porous membrane; a second passage having a second passage portion facing a second area in the second main face of the porous membrane, the second area being positioned backside of the first area; and a first electrode provided in the first passage portion and a second electrode provided in the second passage portion, the first electrode and the second electrode being positioned across the first area and the second area. In the cell evaluation device, tight junctions are formed among the cells by cell cultivation. With the cell evaluation device, any increase in the electric resistance occurring due to the formation of the tight junctions can be easily measured.
    Type: Grant
    Filed: November 20, 2019
    Date of Patent: January 10, 2023
    Assignees: Shimadzu Corporation, Tokyo Institute of Technology
    Inventors: Yoichi Fujiyama, Chikara Miyake, Yoh-ichi Tagawa
  • Publication number: 20220267705
    Abstract: A co-culture apparatus includes: a first airtight container; a co-culture device disposed outside of the first airtight container; a first culture medium source disposed in the first airtight container and storing a first culture medium; a second culture medium source storing a second culture medium having a lower dissolved oxygen concentration than that of the first culture medium; and a first conduit connected to the co-culture device and the first culture medium source. The co-culture device includes: a membrane having a first main surface, and a second main surface opposite to the first main surface for culturing cells; a first flow path partially defined by the first main surface and disposed such that the first culture medium flows therethrough; and a second flow path partially defined by the second main surface and disposed such that the second culture medium flows therethrough. The first flow path has an inlet connected to the first conduit.
    Type: Application
    Filed: February 24, 2022
    Publication date: August 25, 2022
    Applicants: SHIMADZU CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Yoichi FUJIYAMA, Yoh-ichi TAGAWA
  • Publication number: 20220229001
    Abstract: The cell evaluation device includes a cell housing unit 111, and an adapter 120 configured to be elastically deformed by being fitted into the cell housing unit to close a top opening of the cell housing unit, and the adapter includes a body 121 to be inserted into the cell housing unit, a positioning unit 122 configured to position the body in a vertical direction with respect to the cell housing unit, a culture medium inlet port 124 and a culture medium outlet port 125 penetrating the top end and the lower end of the body, and a flexible sheet member 123 which is provided on a lower end of the body and extends radially outward from an outer edge of the lower end. This makes it possible to prevent leakage of the culture medium in the cell evaluation device.
    Type: Application
    Filed: May 1, 2020
    Publication date: July 21, 2022
    Applicant: SHIMADZU CORPORATION
    Inventor: Yoichi FUJIYAMA
  • Patent number: 11142735
    Abstract: To provide a cell treatment container, including: a first member having a flow path in which a cell suspension including a liquid and cells dispersed in the liquid flows through, the flow path formed on a surface of the first member; a second member arranged to face the surface of the first member; and a damming formed in one or both of the first member and the second member, in which the damming is provided with a protrusion part protruding from the first member into the flow path to form a gap for allowing the liquid in the cell suspension to pass through the gap and for damming up the cells in the cell suspension, and a pillar extending from the protrusion part at a first end and being joined to the second member at a second end.
    Type: Grant
    Filed: December 12, 2019
    Date of Patent: October 12, 2021
    Assignee: Shimadzu Corporation
    Inventors: Chikara Miyake, Masaki Kanai, Yoichi Fujiyama, Satoshi Kuramoto
  • Publication number: 20210147777
    Abstract: A co-culture device includes: a first body including a first membrane having a first main surface for culturing cells and a second main surface opposite to the first main surface, a first flow path partially defined by the first main surface, the first flow path configured for a first culture medium to flow therethrough, and a second flow path partially defined by the second main surface, the second flow path configured for a second culture medium having a higher dissolved oxygen concentration than that of the first culture medium to flow therethrough; and an oxygen concentration adjuster for adjusting the dissolved oxygen concentration in the first culture medium to be supplied to the first flow path.
    Type: Application
    Filed: November 18, 2020
    Publication date: May 20, 2021
    Applicants: SHIMADZU CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Yoichi FUJIYAMA, Hiroomi GOTO, Masaki KANAI, Yoh-ichi TAGAWA
  • Patent number: 10962450
    Abstract: This specimen collection device has a channel through which a specimen can be drawn by capillarity. The channel has two channel sections that extend from the tip side to the base side and are connected at the tip side. One of the channel sections is connected to a base side specimen intake port, and the other terminates at a location prior to reaching the base end. An air hole is provided at this terminating location. The specimen collection device is equipped with an extraction unit for collecting a prescribed amount of the specimen. The extraction unit includes the two channel sections on the tip side of the air hole, and can be severed from the other sections of the device body of the specimen collection device via a severable section.
    Type: Grant
    Filed: June 6, 2016
    Date of Patent: March 30, 2021
    Assignee: SHIMADZU CORPORATION
    Inventors: Ippei Takeuchi, Yoichi Fujiyama, Shinobu Kudoh, Koji Inoue
  • Publication number: 20200224138
    Abstract: To provide a cell treatment container, including: a first member having a flow path in which a cell suspension including a liquid and cells dispersed in the liquid flows through, the flow path formed on a surface of the first member; a second member arranged to face the surface of the first member; and a damming formed in one or both of the first member and the second member, in which the damming is provided with a protrusion part protruding from the first member into the flow path to form a gap for allowing the liquid in the cell suspension to pass through the gap and for damming up the cells in the cell suspension, and a pillar extending from the protrusion part at a first end and being joined to the second member at a second end.
    Type: Application
    Filed: December 12, 2019
    Publication date: July 16, 2020
    Inventors: Chikara MIYAKE, Masaki KANAI, Yoichi FUJIYAMA, Satoshi KURAMOTO
  • Publication number: 20200157485
    Abstract: A cell evaluation device includes: a porous membrane having a first main face and a second main face; a first passage having a first passage portion facing a first area on which cells are placed in the first main face of the porous membrane; a second passage having a second passage portion facing a second area in the second main face of the porous membrane, the second area being positioned backside of the first area; and a first electrode provided in the first passage portion and a second electrode provided in the second passage portion, the first electrode and the second electrode being positioned across the first area and the second area. In the cell evaluation device, tight junctions are formed among the cells by cell cultivation. With the cell evaluation device, any increase in the electric resistance occurring due to the formation of the tight junctions can be easily measured.
    Type: Application
    Filed: November 20, 2019
    Publication date: May 21, 2020
    Inventors: Yoichi FUJIYAMA, Chikara MIYAKE, Yoh-ichi TAGAWA
  • Patent number: 10336987
    Abstract: A device for culturing a liver tissue has a culture chamber to which at least two flow channels, which are at least for introducing and discharging a culture medium, are connected. A gel which serves as a cell scaffold material is housed in the culture chamber. A co-culture system containing an endothelial cell-derived cell, a hepatocyte-derived cell and a mesenchymal cell is co-cultured on the gel in such a way that the co-culture system has a tubular structure.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: July 2, 2019
    Assignees: SHIMADZU CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Yoichi Fujiyama, Yoh-ichi Tagawa
  • Publication number: 20190185802
    Abstract: A cell culturing device has: a first culture chamber; a first introduction flow channel and a first discharge flow channel which are connected to the first culture chamber; a second culture chamber connected to a halfway part of the first introduction flow channel via a first porous membrane; and a second introduction flow channel and a second discharge flow channel which are connected to the second culture chamber. The first discharge flow channel is connected to the first culture chamber via a second porous membrane. The first introduction flow channel has a liquid collecting part between the first culture chamber and the second culture chamber.
    Type: Application
    Filed: February 21, 2019
    Publication date: June 20, 2019
    Applicants: SHIMADZU CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Yoichi Fujiyama, Yoh-ichi Tagawa
  • Patent number: 10301586
    Abstract: A cell culturing device has: a first culture chamber; a first introduction flow channel and a first discharge flow channel which are connected to the first culture chamber; a second culture chamber connected to a halfway part of the first introduction flow channel via a first porous membrane; and a second introduction flow channel and a second discharge flow channel which are connected to the second culture chamber. The first discharge flow channel is connected to the first culture chamber via a second porous membrane. The first introduction flow channel has a liquid collecting part between the first culture chamber and the second culture chamber.
    Type: Grant
    Filed: April 14, 2014
    Date of Patent: May 28, 2019
    Assignees: SHIMADZU CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Yoichi Fujiyama, Yoh-ichi Tagawa
  • Publication number: 20190011334
    Abstract: This specimen collection device has a channel through which a specimen can be drawn by capillarity. The channel has two channel sections that extend from the tip side to the base side and are connected at the tip side. One of the channel sections is connected to a base side specimen intake port, and the other terminates at a location prior to reaching the base end. An air hole is provided at this terminating location. The specimen collection device is equipped with an extraction unit for collecting a prescribed amount of the specimen. The extraction unit includes the two channel sections on the tip side of the air hole, and can be severed from the other sections of the device body of the specimen collection device via a severable section.
    Type: Application
    Filed: June 6, 2016
    Publication date: January 10, 2019
    Applicant: SHIMADZU CORPORATION
    Inventors: Ippei TAKEUCHI, Yoichi FUJIYAMA, Shinobu KUDOH, Koji INOUE
  • Publication number: 20170029759
    Abstract: A cell culturing device has: a first culture chamber; a first introduction flow channel and a first discharge flow channel which are connected to the first culture chamber; a second culture chamber connected to a halfway part of the first introduction flow channel via a first porous membrane; and a second introduction flow channel and a second discharge flow channel which are connected to the second culture chamber. The first discharge flow channel is connected to the first culture chamber via a second porous membrane. The first introduction flow channel has a liquid collecting part between the first culture chamber and the second culture chamber.
    Type: Application
    Filed: April 14, 2014
    Publication date: February 2, 2017
    Applicants: SHIMADZU CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Yoichi Fujiyama, Yoh-ichi Tagawa
  • Publication number: 20160281064
    Abstract: A device for culturing a liver tissue has a culture chamber to which at least two flow channels, which are at least for introducing and discharging a culture medium, are connected. A gel which serves as a cell scaffold material is housed in the culture chamber. A co-culture system containing an endothelial cell-derived cell, a hepatocyte-derived cell and a mesenchymal cell is co-cultured on the gel in such a way that the co-culture system has a tubular structure.
    Type: Application
    Filed: November 8, 2013
    Publication date: September 29, 2016
    Applicants: SHIMADZU CORPORATION, TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Yoichi FUJIYAMA, Yoh-ichi TAGAWA
  • Patent number: 8557597
    Abstract: A total organic carbon measuring instrument including a measuring unit composed of, integrated together, organic substance oxidation part and carbon dioxide separation part and conductivity measuring part, control unit and data processing unit. In order to enhance the accuracy of conductivity measurement, the control unit is constructed so as to stop feeding of a sample water at the time of oxidation of organic substance and carry out feeding of the sample water at the time of sample water moving to the organic substance oxidation decomposition part and carbon dioxide separation part. The data processing unit is constructed so as to measure the total organic carbon concentration on the basis of conductivity at the time of arriving of sample water irradiated with ultraviolet rays of which relative intensity is a given value or higher at the carbon dioxide separation part.
    Type: Grant
    Filed: January 6, 2012
    Date of Patent: October 15, 2013
    Assignee: Shimadzu Corporation
    Inventors: Masakazu Akechi, Hirohisa Abe, Yoichi Fujiyama
  • Patent number: 8361414
    Abstract: [PROBLEMS] To increase the rate of gas transfer in gas exchangers. [MEANS FOR SOLVING PROBLEMS] A gas exchange chip comprises substrates (1,2); two flow channels (3,4) formed in the substrates (1,2) and each having an inlet port and an outlet port; and multiple grooves (9) interlinking the flow channels (3,4). The grooves (9) have the size of their sectional area predetermined and have at least a part of the internal surface thereof rendered hydrophobic so as to permit the transfer of gas component while inhibiting any liquid passage. A sample water containing carbon dioxide is caused to flow through one of the flow channels (3) while pure water is caused to flow through the other flow channel (4) to thereby transfer the carbon dioxide contained in the sample water into the pure water.
    Type: Grant
    Filed: December 28, 2005
    Date of Patent: January 29, 2013
    Assignee: Shimadzu Corporation
    Inventors: Masakazu Akechi, Yoichi Fujiyama, Hirohisa Abe, Masaki Kanai
  • Publication number: 20120107947
    Abstract: A total organic carbon measuring instrument including a measuring unit composed of, integrated together, organic substance oxidation part and carbon dioxide separation part and conductivity measuring part, control unit and data processing unit. In order to enhance the accuracy of conductivity measurement, the control unit is constructed so as to stop feeding of a sample water at the time of oxidation of organic substance and carry out feeding of the sample water at the time of sample water moving to the organic substance oxidation decomposition part and carbon dioxide separation part. The data processing unit is constructed so as to measure the total organic carbon concentration on the basis of conductivity at the time of arriving of sample water irradiated with ultraviolet rays of which relative intensity is a given value or higher at the carbon dioxide separation part.
    Type: Application
    Filed: January 6, 2012
    Publication date: May 3, 2012
    Inventors: Masakazu Akechi, Hirohisa Abe, Yoichi Fujiyama
  • Patent number: 7993586
    Abstract: Disclosed is a total organic carbon (TOC) measurement apparatus capable of facilitating reduction in size of the apparatus and achieving enhanced measurement accuracy. The TOC measurement apparatus includes an organics oxidation unit, a carbon-dioxide separation unit and a conductivity measurement unit, which are integrally formed by laminating a plurality of plates to define therebetween and incorporate therein an oxidizing flow passage, an aqueous-sample flow passage, a gas permeable membrane, a measurement-water flow passage and a conductivity measuring electrode. The TOC measurement apparatus is designed such that carbon oxide in an aqueous sample is transferred from the aqueous-sample flow passage to the measurement-water flow passage through the gas permeable membrane, and a conductivity of measurement water which contains the carbon oxide and flows through a flow passage facing the conductivity measuring electrode is determined and converted to a TOC content.
    Type: Grant
    Filed: June 28, 2007
    Date of Patent: August 9, 2011
    Assignee: Shimadzu Corporation
    Inventors: Yoichi Fujiyama, Masakazu Akechi, Masaki Kanai
  • Patent number: 7931865
    Abstract: An apparatus which comprises: an organic-substance oxidation part in which organic substances contained in a sample water supplied are oxidized into carbon dioxide; a carbon dioxide separation part in which the carbon dioxide contained in the sample water is caused to permeate and come into a measurement water; and a conductivity measurement part in which the conductivity of the measurement water is measured. The carbon dioxide separation part comprises: a sample water channel in which sample water flows; an intermediate water part in which intermediate water having a higher pH value in a neutral region than the sample water is present; and a measurement water channel through which the measurement water comprising deionized water flows. The sample water channel is in contact with the intermediate water part through a gas-permeable membrane, and the intermediate water part is in contact with the measurement water channel through a gas-permeable membrane.
    Type: Grant
    Filed: October 17, 2006
    Date of Patent: April 26, 2011
    Assignee: Shimadzu Corporation
    Inventors: Yoichi Fujiyama, Hirohisa Abe, Masakazu Akechi, Takeaki Inoue, Takeshi Iharada