Patents by Inventor Yoichi Kumada
Yoichi Kumada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220127304Abstract: Provided is a separating agent that does not have a significantly reduced dynamic binding capacity (DBC) to a target substance even when the separating agent is continually CIP-treated under alkaline pH conditions. The separating agent includes a carrier and a protein, wherein the protein is a given protein, and a surface of the carrier and a lysine residue in the protein are bound by a chemical bond.Type: ApplicationFiled: July 1, 2019Publication date: April 28, 2022Applicants: NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY, DAICEL CORPORATIONInventors: Yoichi KUMADA, Hiromichi OKURA, Seiichi UCHIMURA
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Patent number: 11136609Abstract: An object of the present invention is to provide a separation agent for separating a human serum-derived IgG polyclonal antibody. This object is achieved by a separation agent for separating a human serum-derived IgG polyclonal antibody, the separation agent including: a carrier; and a single-chain antibody which has a dissociation constant for a human serum-derived IgG polyclonal antibody of not more than 3.0×10?8 M and which binds to the surface of the carrier via a chemical bond.Type: GrantFiled: November 7, 2016Date of Patent: October 5, 2021Assignees: NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY, DAICEL CORPORATIONInventors: Yoichi Kumada, Yuya Hasegawa, Seiichi Uchimura
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Publication number: 20210269476Abstract: Provided is a separating agent with an improved dynamic binding capacity (DBC) to a target substance. The separating agent includes a carrier and a protein, wherein the protein is a given protein, and a surface of the carrier and added lysine residues in the protein are bound by chemical bonds.Type: ApplicationFiled: July 1, 2019Publication date: September 2, 2021Applicants: NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY, DAICEL CORPORATIONInventors: Yoichi KUMADA, Hiromichi OKURA, Seiichi UCHIMURA
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Patent number: 11078254Abstract: Provided is a highly sensitive and less expensive lectin-immobilized base material (for example, a lectin plate), such as lectin-immobilized base material having stable qualities and being able to be sufficiently washed after a target sugar chain-containing antigen binds thereto. Further provided is a method for immobilizing lectin to a base material therefor. Particularly provided are: a method whereby a lectin-peptide fusion, in which a peptide capable of adsorbing to a base material surface such as a polystyrene (PS) tag is fused with the N-terminal side or C-terminal side of lectin capable of recognizing a target sugar chain, is immobilized on the peptide side to a base material; and a lectin-immobilized base material produced by this method. By using the lectin-immobilized base material, a target sugar chain-containing antigen can be highly sensitively and evenly measured and, moreover, target sugar chain-containing cells, etc. can be separated (concentrated and harvested).Type: GrantFiled: April 11, 2018Date of Patent: August 3, 2021Assignees: National Institute of Advanced Industrial Science and Technology, Nissan Chemical Corporation, National University Corporation Kyoto Institute of TechnologyInventors: Hiroaki Tateno, Junko Katayama, Kazutaka Matoba, Yoichi Kumada
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Publication number: 20200123222Abstract: A purpose of the present invention is to provide a highly sensitive and less expensive lectin-immobilized base material (for example, a lectin plate), said lectin-immobilized base material having stable qualities and being able to be sufficiently washed after a target sugar chain-containing antigen binds thereto. Another purpose of the present invention is to provide a method for immobilizing lectin to a base material therefor. Provided are: a method whereby a lectin-peptide fusion, in which a peptide capable of adsorbing to a base material surface such as a PS tag is fused with the N-terminal side or C-terminal side of lectin capable of recognizing a target sugar chain, is immobilized on the peptide side to a base material; and a lectin-immobilized base material produced by this method. By using the lectin-immobilized base material, a target sugar chain-containing antigen can be highly sensitively and evenly measured and, moreover, target sugar chain-containing cells, etc.Type: ApplicationFiled: April 11, 2018Publication date: April 23, 2020Inventors: Hiroaki Tateno, Junko Katayama, Kazutaka Matoba, Yoichi Kumada
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Patent number: 10520486Abstract: Provided is a novel method of suppressing non-specific protein adsorption. The above-mentioned object can be achieved by treating a solid phase with a solid phase-treating liquid containing a phosphorylcholine group-containing polymer as a main component, and treating a specimen with a specimen-treating liquid containing, as a main component, a polymer having a phosphorylcholine group, a hydroxy group, and a hydrophobic group, followed by addition of the treated specimen to the treated solid phase.Type: GrantFiled: February 18, 2016Date of Patent: December 31, 2019Assignees: National University Corporation Kyoto Institute of Technology, NOF CorporationInventors: Yoichi Kumada, Yasutaka Kawaguchi, Aranna Nemoto, Fumio Nakashima, Nobuyuki Sakamoto
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Publication number: 20180327803Abstract: An object of the present invention is to provide a separation agent for separating a human serum-derived IgG polyclonal antibody. This object is achieved by a separation agent for separating a human serum-derived IgG polyclonal antibody, the separation agent including: a carrier; and a single-chain antibody which has a dissociation constant for a human serum-derived IgG polyclonal antibody of not more than 3.0×10?8 M and which binds to the surface of the carrier via a chemical bond.Type: ApplicationFiled: November 7, 2016Publication date: November 15, 2018Applicants: NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY, DAICEL CORPORATIONInventors: Yoichi KUMADA, Yuya HASEGAWA, Seiichi UCHIMURA
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Publication number: 20180031537Abstract: Provided is a novel method of suppressing non-specific protein adsorption. The above-mentioned object can be achieved by treating a solid phase with a solid phase-treating liquid containing a phosphorylcholine group-containing polymer as a main component, and treating a specimen with a specimen-treating liquid containing, as a main component, a polymer having a phosphorylcholine group, a hydroxy group, and a hydrophobic group, followed by addition of the treated specimen to the treated solid phase.Type: ApplicationFiled: February 18, 2016Publication date: February 1, 2018Applicants: NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY, NOF CORPORATIONInventors: Yoichi Kumada, Yasutaka KAWAGUCHI, Aranna NEMOTO, Fumio NAKASHIMA, Nobuyuki SAKAMOTO
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Publication number: 20180022830Abstract: An object of the present invention is to provide a polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane (PDMS) is bound; a method for immobilizing a target protein on a polydimethylsiloxane substrate; a peptide having an affinity for PDMS; a polynucleotide encoding a peptide having an affinity for PDMS; and a vector using thereof. A polydimethylsiloxane substrate to which a peptide having an affinity for polydimethylsiloxane is bound, the peptide comprising the following peptide (1a) or (1b), or a fragment thereof: (1a) a peptide comprising an amino acid sequence represented by at least one member selected from the group consisting of SEQ ID NOs: 1 to 9; and (1b) a peptide comprising the amino acid sequence of (1a) in which one or more amino acids are deleted, substituted, and/or added, the peptide having an affinity for polydimethylsiloxane.Type: ApplicationFiled: February 12, 2016Publication date: January 25, 2018Inventors: Yoichi KUMADA, Ryoko OTSUKI, Ryota AKAI, Junko KATAYAMA, Kazutaka MATOBA
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Patent number: 9850316Abstract: A method for refolding an antibody, a process for producing a refolded antibody, a refolded antibody, and uses thereof are provided. A method for refolding an antibody in a liquid phase comprises the steps of denaturing an inactive antibody binding directly or through a linker to a peptide, the peptide having an isoelectric point lower than the isoelectric point of the inactive antibody, and dispersing in a liquid phase the peptide-binding inactive antibody denatured in the step above. Also provided is a process for producing a refolded antibody.Type: GrantFiled: February 3, 2014Date of Patent: December 26, 2017Assignee: National University Corporation Kyoto Institute of TechnologyInventors: Yoichi Kumada, Yasuyuki Ishikawa, Yusuke Fujiwara, Michimasa Kishimoto
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Patent number: 9701760Abstract: The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride.Type: GrantFiled: February 12, 2013Date of Patent: July 11, 2017Assignee: National University Corporation Kyoto Institute of TechnologyInventors: Yoichi Kumada, Michimasa Kishimoto, Takeru Otsuka
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Patent number: 9598501Abstract: The present invention provides an antibody-immobilized carrier that can be used in antibody screening, a method of producing the antibody-immobilized carrier, and use of the antibody-immobilized carrier. Efficient antibody screening can be carried out particularly by an antibody-immobilized carrier including two or more antibody immobilized regions onto each of which a heavy-chain low-molecular-weight antibody and a light-chain low-molecular-weight antibody are separately immobilized, the two or more antibody immobilized regions each being included in an independent manner, the heavy-chain low-molecular-weight antibody including a heavy-chain variable region, the light-chain low-molecular-weight antibody including a light-chain variable region, the heavy-chain low-molecular-weight antibody and the light-chain low-molecular-weight antibody each being derived from an antibody recognizing a different antigen.Type: GrantFiled: September 30, 2014Date of Patent: March 21, 2017Assignees: NATIONAL UNIVERSITY CORPORATION KYOTO INSTITUTE OF TECHNOLOGY, DENKA SEIKEN CO., LTDInventors: Yoichi Kumada, Michimasa Kishimoto, Kyoko Hamasaki, Aya Nakagawa
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Publication number: 20150376297Abstract: A method for refolding an antibody, a process for producing a refolded antibody, a refolded antibody, and uses thereof are provided. A method for refolding an antibody in a liquid phase comprises the steps of denaturing an inactive antibody binding directly or through a linker to a peptide, the peptide having an isoelectric point lower than the isoelectric point of the inactive antibody, and dispersing in a liquid phase the peptide-binding inactive antibody denatured in the step above. Also provided is a process for producing a refolded antibody.Type: ApplicationFiled: February 3, 2014Publication date: December 31, 2015Inventors: Yoichi Kumada, Yasuyuki Ishikawa, Yusuke Fujiwara, Michimasa Kishimoto
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Publication number: 20150038675Abstract: The purpose of the present invention is to provide: a peptide having an affinity for silicon nitride; a polynucleotide encoding the peptide; an expression vector for expressing the peptide having an affinity for silicon nitride; an expression vector for expressing a peptide fusion protein that comprises the peptide having an affinity for silicon nitride and a target protein; a transformant obtained by introducing the expression vector into a host cell; a peptide fusion protein obtained from the transformant; a silicon nitride substrate to which a peptide having an affinity for silicon nitride has been bonded; a method for immobilizing a target protein to a silicon nitride substrate; a composition for immobilizing a target protein to a silicon nitride substrate, the composition comprising a peptide having an affinity for silicon nitride; and a linker for immobilizing a target protein to a silicon nitride substrate, the linker comprising a peptide having an affinity for silicon nitride.Type: ApplicationFiled: February 12, 2013Publication date: February 5, 2015Inventors: Yoichi Kumada, Michimasa Kishimoto, Takeru Otsuka
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Publication number: 20150018534Abstract: The present invention provides an antibody-immobilized carrier that can be used in antibody screening, a method of producing the antibody-immobilized carrier, and use of the antibody-immobilized carrier. Efficient antibody screening can be carried out particularly by an antibody-immobilized carrier including two or more antibody immobilized regions onto each of which a heavy-chain low-molecular-weight antibody and a light-chain low-molecular-weight antibody are separately immobilized, the two or more antibody immobilized regions each being included in an independent manner, the heavy-chain low-molecular-weight antibody including a heavy-chain variable region, the light-chain low-molecular-weight antibody including a light-chain variable region, the heavy-chain low-molecular-weight antibody and the light-chain low-molecular-weight antibody each being derived from an antibody recognizing a different antigen.Type: ApplicationFiled: September 30, 2014Publication date: January 15, 2015Inventors: Yoichi Kumada, Michimasa Kishimoto, Kyoko Hamasaki, Aya Nakagawa
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Patent number: 8883985Abstract: The present invention provides an antibody-immobilized carrier that can be used in antibody screening, a method of producing the antibody-immobilized carrier, and use of the antibody-immobilized carrier. Efficient antibody screening can be carried out particularly by an antibody-immobilized carrier including two or more antibody immobilized regions onto each of which a heavy-chain low-molecular-weight antibody and a light-chain low-molecular-weight antibody are separately immobilized, the two or more antibody immobilized regions each being included in an independent manner, the heavy-chain low-molecular-weight antibody including a heavy-chain variable region, the light-chain low-molecular-weight antibody including a light-chain variable region, the heavy-chain low-molecular-weight antibody and the light-chain low-molecular-weight antibody each being derived from an antibody recognizing a different antigen.Type: GrantFiled: February 15, 2011Date of Patent: November 11, 2014Assignees: National University Corporation Kyoto Institute of Technology, Denka Seiken Co., Ltd.Inventors: Yoichi Kumada, Michimasa Kishimoto, Kyoko Hamasaki, Aya Nakagawa
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Publication number: 20140220626Abstract: Provided is a peptide containing a variable region and improved in production efficiency. The peptide contains a variable region to which an antigen-binding site is to be formed and has an amino acid sequence expressing a specific adsorption function to a solid phase at a site closer to the C-terminal than a heavy-chain variable region or at a site closer to the C-terminal than a light-chain variable region.Type: ApplicationFiled: November 22, 2013Publication date: August 7, 2014Applicants: National University Corporation Kyoto Institute of Technology, ENPLAS CORPORATIONInventors: Mitsuyasu Koike, Kyoko Hamasaki, Yuki Shiritani, Michimasa Kishimoto, Yoichi Kumada, Takuhito Ohse
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Patent number: 8618247Abstract: Provided is a peptide containing a variable region and improved in production efficiency. The peptide contains a variable region to which an antigen-binding site is to be formed and has an amino acid sequence expressing a specific adsorption function to a solid phase at a site closer to the C-terminal than a heavy-chain variable region or at a site closer to the C-terminal than a light-chain variable region.Type: GrantFiled: February 12, 2009Date of Patent: December 31, 2013Assignees: Enplas Corporation, National University Corporation Kyoto Institute of TechnologyInventors: Yoichi Kumada, Michimasa Kishimoto, Yuki Shiritani, Kyoko Hamasaki, Takuhito Ohse, Mitsuyasu Koike
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Publication number: 20120309943Abstract: The present invention provides an antibody-immobilized carrier that can be used in antibody screening, a method of producing the antibody-immobilized carrier, and use of the antibody-immobilized carrier. Efficient antibody screening can be carried out particularly by an antibody-immobilized carrier including two or more antibody immobilized regions onto each of which a heavy-chain low-molecular-weight antibody and a light-chain low-molecular-weight antibody are separately immobilized, the two or more antibody immobilized regions each being included in an independent manner, the heavy-chain low-molecular-weight antibody including a heavy-chain variable region, the light-chain low-molecular-weight antibody including a light-chain variable region, the heavy-chain low-molecular-weight antibody and the light-chain low-molecular-weight antibody each being derived from an antibody recognizing a different antigen.Type: ApplicationFiled: February 15, 2011Publication date: December 6, 2012Inventors: Yoichi Kumada, Michimasa Kishimoto, Kyoko Hamasaki, Aya Nakagawa
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Publication number: 20110065149Abstract: Described herein is a polynucleotide encoding a fusion protein comprising two or more polypeptide domains and a polypeptide linker joining the domains, wherein the sequence of the polynucleotide encoding the polypeptide linker is selected such that when the mRNA transcribed from the polynucleotide is translated in a host cell transfected with the polynucleotide, the translation rate of the mRNA region encoding the polypeptide linker is slower than the translation rate of the mRNA region encoding the polypeptide domain immediately upstream thereof. Also provided are a vector transfected with the polynucleotide of the present invention so that the polynucleotide can be expressed in the host cell; a host cell transformed by that vector; and a process for producing a fusion protein comprising culturing the host cell, and recovering the fusion protein thus produced.Type: ApplicationFiled: August 21, 2007Publication date: March 17, 2011Applicants: National University Corporation Kobe University, Chugai Seiyaku Kabushiki KaishaInventors: Shigeo Kato, Yoichi Kumada, Tomomi Kawasaki, Yasufumi Kikuchi