Patents by Inventor Yoichi Uosaki
Yoichi Uosaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7112432Abstract: An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.Type: GrantFiled: June 10, 2004Date of Patent: September 26, 2006Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Publication number: 20040229337Abstract: An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.Type: ApplicationFiled: June 10, 2004Publication date: November 18, 2004Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 6767726Abstract: An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.Type: GrantFiled: May 6, 2002Date of Patent: July 27, 2004Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Publication number: 20030092153Abstract: An isolated gene encodes for a L-proline-3-hydroxylase having the amino acid sequence of SEQ ID NOS: 1, 2, 15, 16 and 17, or for a protein having enzymatic activity to hydroxylate the 3-position of L-proline and to act on free L-proline in the presence of 2-ketoglutaric acid and divalent iron ions to produce cis-3-hydroxy-L-proline. The gene is inserted into a vector and the resulting recombinant DNA is used to create a transformant. L-proline-3-hydroxylase is produced by culturing the transformant in a medium.Type: ApplicationFiled: May 6, 2002Publication date: May 15, 2003Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 6413748Abstract: An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.Type: GrantFiled: December 6, 1999Date of Patent: July 2, 2002Assignee: Kyowa Hakko Kogyo, Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 6010891Abstract: An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant, is also provided.Type: GrantFiled: April 7, 1999Date of Patent: January 4, 2000Assignee: Kyowa Hakko Kogyo, Co, Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 5962292Abstract: An industrially applicable process for producing cis-3-hydroxy-L-proline, which is useful as a raw material for medicines or as an additive to foods. In the process, L-proline is converted into cis-3-hydroxy-L-proline in the presence of an enzyme source which is derived from a microorganism belonging to the genus Streptomyces or Bacillus and which catalyzes hydroxylation of L-proline into cis-3-hydroxy-L-proline, a divalent iron ion and 2-ketoglutaric acid, in an aqueous medium, and the produced cis-3-hydroxy-L-proline is collected from the aqueous medium. A novel enzyme L-proline-3-hydroxylase, a gene of L-proline-3-hydroxylase which is useful for the process, a transformant containing the gene, and a process for producing L-proline-3-hydroxylase using the transformant.Type: GrantFiled: September 9, 1996Date of Patent: October 5, 1999Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Akio Ozaki, Hideo Mori, Takeshi Shibasaki, Katsuhiko Ando, Keiko Ochiai, Shigeru Chiba, Yoichi Uosaki
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Patent number: 5189187Abstract: A clerodane-type diterpene derivative, which has antibacterial and antitumor activities, as well as chemically equivalent tautomers thereof and a process for producing said derivative using a microorganism belonging to the genus Streptomyces.Type: GrantFiled: June 24, 1992Date of Patent: February 23, 1993Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hirofumi Nakano, Shozo Kawada, Yoichi Uosaki, Yutaka Saito, Katsushige Gomi, Toshiaki Iwazaki
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Patent number: 5151352Abstract: A clerodane-type diterpene derivative, which has antibacterial and antitumor activities, as well as chemically equivalent tautomers thereof and a process for producing said derivative using a microorganism belonging to the genus Streptomyces.Type: GrantFiled: September 30, 1991Date of Patent: September 29, 1992Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hirofumi Nakano, Shozo Kawada, Yoichi Uosaki, Yutaka Saito, Katsushige Gomi, Toshiaki Iwazaki
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Patent number: 5117006Abstract: A DC-88A derivative represented by general formula: ##STR1## wherein ##STR2## represents ##STR3## has an excellent antitumor activity and is useful as an anti-tumor agent.Type: GrantFiled: May 15, 1991Date of Patent: May 26, 1992Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hiromitsu Saito, Masaji Kasai, Makoto Morimoto, Eiji Kobayashi, Yoichi Uosaki, Yutaka Kanda, Hiroshi Sano
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Patent number: 5084468Abstract: A novel DC-88A derivative represented by general formula: ##STR1## wherein ##STR2## represents ##STR3## has an excellent antitumor activity and is useful as an antitumor agent.Type: GrantFiled: August 10, 1989Date of Patent: January 28, 1992Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hiromitsu Saito, Masaji Kasai, Makoto Morimoto, Eiji Kobayashi, Yoichi Uosaki, Yutaka Kanda, Hiroshi Sano
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Patent number: 4946957Abstract: The present invention relates to novel derivatives of DC-52 represented by the following formula (I): ##STR1## [wherein X is chlorine, bromine, iodine, hydroxyl, formyl, hydroxyiminomethyl, cyano, nitro, amino or lower alkanoylamino; and Y is hydroxyl and Z is cyano, or Y and Z represent --O-- in the form of --Y--Z--]. These compounds and pharmacologically acceptable salts thereof have high anti-tumor activity.Type: GrantFiled: August 30, 1989Date of Patent: August 7, 1990Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hiromitsu Saito, Yoichi Uosaki, Akira Sato, Tadashi Hirata, Makoto Morimoto, Tadashi Ashizawa
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Patent number: 4879386Abstract: The present invention relates to novel derivatives of DC-52 represented by the following formula (I): ##STR1## ps [wherein X is chlorine, bromine, iodine, hydroxyl, formyl, hydroxyiminomethyl, cyano, nitro, amino or lower alkanoylamino; and Y is hydroxyl and Z is cyano, or Y and Z represent --O-- in the form of --Y--Z--]. These compounds and pharmacologically acceptable salts thereof have high anti-tumor activity.Type: GrantFiled: May 25, 1988Date of Patent: November 7, 1989Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hiromitsu Saito, Yoichi Uosaki, Akira Sato, Tadashi Hirata, Makoto Morimoto, Tadashi Ashizawa
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Patent number: 4822882Abstract: Compounds represented by the following formula or by the corresponding hydroquinone type formula: ##STR1## in which R.sup.1 and R.sup.2 are independently hydrogen, lower alkoxy, azido, amino, lower alkylamino, di-lower alkylamino, cyclic amino, lower alkylthio or unsubstituted or substituted arylthio; and X represents hydroxyl and Y represents cyano or X and Y together represents --O-- in the form of --X--Y--, have an excellent antitumor activity.Type: GrantFiled: September 29, 1987Date of Patent: April 18, 1989Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Hiromitsu Saito, Yoichi Uosaki, Akira Sato, Tadashi Hirata, Makoto Morimoto, Tadashi Ashizawa