Patents by Inventor Yoji Tsukada

Yoji Tsukada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Epimerase
    Patent number: 5994105
    Abstract: A novel polypeptide, acylglucosamine 2-epimerase as shown in FIG. 1 and derivatives thereof, DNA coding for said enzyme, a recombinant vector containing said enzyme, a transformant integrated thereinto said vector, a method for producing said enzyme and a method for producing N-acetylmannosamine and N-acetylneuraminic acid using renin binding protein.
    Type: Grant
    Filed: January 13, 1998
    Date of Patent: November 30, 1999
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiro Ohta, Isafumi Maru
  • Method for assaying sulfate-conjugated bile acid and therefore
    Patent number: 5919644
    Abstract: In a method of quantifying sulfate-conjugated bile acid in a sample with bile acid sulfate sulfatase and a reductive system indicator, an 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt is used as a reductive system indicator. Sulfate-conjugated bile acid can be quantified through a single reaction without complication.
    Type: Grant
    Filed: August 21, 1997
    Date of Patent: July 6, 1999
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Kenichi Adachi, Yasuhiko Tazuke, Yoji Tsukada
  • Epimerase
    Patent number: 5795767
    Abstract: A novel polypeptide, acylglucosamine 2-epimerase as shown in FIG. 1 and derivatives thereof, DNA coding for said enzyme, a recombinant vector containing said enzyme, a transformant integrated thereinto said vector, a method for producing said enzyme and a mrthod for producing N-acetylmannosamine and N-acetylneuraminic acid using renin binding protein.
    Type: Grant
    Filed: November 22, 1995
    Date of Patent: August 18, 1998
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiro Ohta, Isafumi Maru
  • Method for preparing N-acetylneuraminic acid
    Patent number: 5665574
    Abstract: A method for preparing N-acetylneuraminic acid characterized in reacting N-acetylglucosamine with pyruvic acid in an alkaline condition by the action of N-acetylneuraminic acid lyase.
    Type: Grant
    Filed: September 14, 1995
    Date of Patent: September 9, 1997
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiro Ohta
  • Sulfate of n-acetylneuraminic acid homopolymer and processes for making and using the same
    Patent number: 5637690
    Abstract: A sulfate ester of N-acetylneuraminic acid homopolymer represented by the formula (I) or a pharmaceutically acceptable salt thereof: ##STR1## wherein R represents H or SO.sub.3 H, n is 5-1,000, wherein the number of SO.sub.3 H residues per 1 molecule of N-acetylneuraminic acid residues is 0.1 to 3.0, which sulfate ester demonstrates anti-HIV activity.
    Type: Grant
    Filed: December 27, 1994
    Date of Patent: June 10, 1997
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Jang-ho Lee, Yasuhiro Ota, Yoji Tsukada
  • Bile acid sulfate sulfatase gene, plasmid containing said gene and method of producing bile acid sulfate sulfatase
    Patent number: 5585255
    Abstract: The present invention relates to a bile acid sulfate sulfatase gene coding for the amino acid sequence as shown in FIG. 4 and the gene derived from bacteria which belongs to genus Pseudomonas testosteroni, or a bile acid sulfate sulfatase gene as shown in FIG. 3, a plasmid containing the gene, a method for producing a bile acid sulfate sulfatase, and a bile acid sulfate sulfatase.
    Type: Grant
    Filed: October 26, 1993
    Date of Patent: December 17, 1996
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiko Tazuke, Shigenori Okada, Kenichi Adachi
  • Method for preparing N-acetylneuraminic acid by N-acetylneuraminic acid lysase at a pH of 10-12
    Patent number: 5472860
    Abstract: A method for preparing N-acetylneuraminic acid characterized in reacting N-acetylglucosamine with pyruvic acid at an alkaline pH in the presence of N-acetylneuraminic acid lyase.
    Type: Grant
    Filed: September 30, 1993
    Date of Patent: December 5, 1995
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Yoji Tsukada, Yasuhiro Ohta
  • Process for producing ganglioside G.sub.M1
    Patent number: 5296360
    Abstract: The present invention provides a process for preparing ganglioside G.sub.Ml characterized in that neuraminidase isozyme S is allowed to act on gangliosides to produce ganglioside G.sub.Ml.
    Type: Grant
    Filed: June 28, 1991
    Date of Patent: March 22, 1994
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiro Ohta
  • Process for producing asialo GM1
    Patent number: 5275939
    Abstract: The present invention provides a process for preparing asialo G.sub.M1 characterized in that neuraminidase isozyme L is allowed to act on gangliosides to produce asialo G.sub.M1.
    Type: Grant
    Filed: June 28, 1991
    Date of Patent: January 4, 1994
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiro Ohta
  • Process for preparing neuraminidase
    Patent number: 5116752
    Abstract: The present invention provides a process for preparing neuraminidase comprising incubating a mutant of the genus Arthrobacter capable of producing neuraminidase in the absence of any inducing substance, and recovering neuraminidase from the resulting culture.
    Type: Grant
    Filed: January 15, 1991
    Date of Patent: May 26, 1992
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiro Ohta
  • Bile acid sulfate sulfatase, process for its preparation and method for assaying bile acid
    Patent number: 5100795
    Abstract: The invention provides a novel bile acid sulfate sulfatase, a process for its preparation, and a method of assaying bile acid 3.alpha.-sulfates and total bile acids using the bile acid sulfatase.
    Type: Grant
    Filed: April 15, 1991
    Date of Patent: March 31, 1992
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiko Tatsuke
  • Bile acid sulfate sulfatase, process for its preparation and method for assaying bile acid
    Patent number: 5091305
    Abstract: The invention provides a novel bile acid sulfate sulfatase, a process for its preparation, and a method of assaying bile acid 3.alpha.-sulfates and total bile acids using the bile acid sulfatase.
    Type: Grant
    Filed: July 26, 1990
    Date of Patent: February 25, 1992
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yasuhiko Tatsuke
  • Process for producing N-acylneuraminate aldolase
    Patent number: 4699883
    Abstract: This invention provides a process for producing N-acylneuraminate aldolase comprising incubating a mutant of the genus Escherichia capable of producing N-acylneuraminate aldolase even in the absence of any inducing substance, and collecting N-acylneuraminate aldolase from the resulting culture.
    Type: Grant
    Filed: July 23, 1985
    Date of Patent: October 13, 1987
    Assignee: Marukin Shoyu Co., Ltd.
    Inventors: Tsunetake Sugimori, Yoji Tsukada, Yoshihiro Uchida, Yasuhiro Ohta