Abstract: A method for generating a three-dimensional luminescence image includes setting a focal interval between two-dimensional images in accordance with localization of luminescence in a three-dimensional sample. The three-dimensional sample contains a plurality of cells prepared to be luminescent and has a three-dimensional shape. The two-dimensional images have mutually different focal planes and are acquired at the focal interval. The method further includes acquiring a two-dimensional image set including two-dimensional images at the focal interval that is set by imaging the three-dimensional sample under an unirradiated condition; and generating a three-dimensional luminescence image by combining the two-dimensional images included in the two-dimensional image set together.

Abstract: A method for monitoring differentiation into cardiac muscle cells includes keeping, in an alive state, cells into which a reporter gene of luminescent protein configured to vary in luminescence intensity according to an expression of myocardial differentiation marker gene is introduced. The method includes acquiring a luminescence image as a still image by imaging light emitted from the cells in a light shielding state. The method includes acquiring sequential images with illuminating the cells. The method includes associating biological information obtained from the sequential images with biological information obtained from the still image.

Abstract: A method for generating a three-dimensional luminescence image includes setting a focal interval between two-dimensional images in accordance with localization of luminescence in a three-dimensional sample. The three-dimensional sample contains a plurality of cells prepared to be luminescent and has a three-dimensional shape. The two-dimensional images have mutually different focal planes and are acquired at the focal interval. The method further includes acquiring a two-dimensional image set including two-dimensional images at the focal interval that is set by imaging the three-dimensional sample under an unirradiated condition; and generating a three-dimensional luminescence image by combining the two-dimensional images included in the two-dimensional image set together.

Abstract: A method for monitoring differentiation into cardiac muscle cells includes keeping, in an alive state, cells into which a reporter gene of luminescent protein configured to vary in luminescence intensity according to an expression of myocardial differentiation marker gene is introduced. The method includes acquiring a luminescence image as a still image by imaging light emitted from the cells in a light shielding state. The method includes acquiring sequential images with illuminating the cells. The method includes associating biological information obtained from the sequential images with biological information obtained from the still image.

Abstract: A method for monitoring the differentiation state of a stem cell, includes a step (A-1) of culturing a stem cell into which a fusion gene of a promoter region of a differentiation state detection marker gene and a luminescent protein-coding gene is introduced, a step (A-2) of culturing the stem cell under a differentiation-inducing condition after the step (A-1), and a step (A-3) of capturing images of luminescence emitted by expression of the luminescent protein-coding gene in the stem cell, over at least a given period of the steps (A-1) to (A-2).

Abstract: A potentiometric titration method including calculating a titration curve and a differential curve from measured values; computing a straight line (D) connecting the two measured values on the titration curve which are present adjacent to a tentative terminal point (P); computing a first estimation line (B1) and a second estimation line (B2) by a least squares method based on a set of the measured values (d1), (d2) and (d3) and a set of the measured values (d4), (d5) and (d6), respectively, on the titration curve, which are respectively spaced by a predetermined level apart from the tentative terminal point (P) by using the number of decision points (n) as an index where the number of decision points (n) represents the number of measured values on the previously set titration curve; computing an intermediate line (C) between the first estimation line (B1) and the second estimation line (B2); and computing an intersection point (A) of the intermediate line (C) and the straight line (D) to read out a value of t

Abstract: An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples.

Abstract: An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples.

Abstract: An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples.

Abstract: There is provided a novel luminescence measuring apparatus that enables measuring a luminescence amount or a luminescence intensity from plural living biological samples, such as tissues, cells, and biological individuals, into which a gene expressing a luminescent protein has been introduced, individually for the respective biological samples. The inventive luminescence measuring apparatus comprises an image acquisition portion that acquires a luminescence image of biological samples; a measurement region determination portion that determines at least one measurement region in the luminescence image; and a luminescence measurement portion that measures the luminescence amount or luminescence intensity in the determined measurement region; thereby measuring the luminescence amount or luminescence intensity individually by the biological sample(s) included in the measurement region. Irrespective of an efficiency of transfer of a luminescent protein gene into a cell, etc.

Abstract: The present invention relates to a potentiometric titration method comprising assuming a titration curve and a differential curve from measured values; computing a straight line (D) connecting the two measured values on the titration curve which are present adjacent to a tentative terminal point (P); computing a first estimation line (B1) and a second estimation line (B2) by a least squares method based on a set of the measured values (d1), (d2) and (d3) and a set of the measured values (d4), (d5) and (d6), respectively, on the titration curve, which are respectively spaced by a predetermined level apart from the tentative terminal point (P) by using the number of decision points (n) as an index wherein the number of decision points (n) represents the number of measured values on the previously set titration curve; computing an intermediate line (C) between the first estimation line (B1) and the second estimation line (B2); and computing an intersection point (A) of the intermediate line (C) and the straight

Abstract: An object of the present invention is to provide a predetermined site luminescence measuring method and a predetermined site luminescence measuring apparatus, which allow for determining whether, when the luminescence from the predetermined site in live samples is measured, a photoprotein is localized at the predetermined site in the same ones as the samples.

Abstract: A fluorescence reader of the present invention detects fluorescence from a sample present on a carrier or in a solution, and the fluorescence reader includes a light source which radiates parallel light, a projection lens which converges the light from the light source, an objective lens which irradiates the sample with the light converged in a rear-side focal position, an image forming lens which forms fluorescence emitted from the sample and passed through the objective lens into an image, a light receiving pinhole disposed in an image forming position of the image forming lens, and detector which detects the fluorescence passed through the light receiving pinhole.

Abstract: A fluorescence reader of the present invention detects fluorescence from a sample present on a carrier or in a solution, and the fluorescence reader includes a light source which radiates parallel light, a projection lens which converges the light from the light source, an objective lens which irradiates the sample with the light converged in a rear-side focal position, an image forming lens which forms fluorescence emitted from the sample and passed through the objective lens into an image, a light receiving pinhole disposed in an image forming position of the image forming lens, and detector which detects the fluorescence passed through the light receiving pinhole.

Abstract: The present invention discloses a probe array for detection comprising at least one stereo-substrate and plural kinds of probes immobilized on the surface of the stereo-substrate, wherein the probes are respectively and specifically bonded with target materials which differ every kind.