Abstract: Provided herein are methods of producing lipid membrane bound particles, such as lentiviral vectors, that contain one or more Paramyxovirus envelope proteins in the lipid bilayer. Also provided are lipid membrane bound particles containing one or more Paramyxovirus envelope proteins in the lipid bilayer. In some embodiments, the one or more Paramyxovirus envelope protein is a Nipah virus (NiV) protein G or F or a biologically active portion or retargeted fusion thereof. In some embodiments, the lipid membrane bound particle is a lentiviral vector. Also provided are related compositions, kits and systems in connection with the provided methods.

Abstract: The present disclosure provides methods and reagents for culturing pluripotent stem cells. The method comprises a first step of culturing in a medium comprising inhibitors of GSK-3?, JNK, p38, PKC and Erk1/2, followed by a second step of culturing in a medium comprising inhibitors of GSK-3?, JNK, p38 and PKC, and not comprising an inhibitor of Erk1/2. The medium of the second step may also further comprise dextran sulfate. The pluripotent stem cells produced using the methods and reagents provided may also be differentiated into a cell type of interest. The methods and reagents provided in this disclosure offer robust and scalable technologies for manufacturing the quantities of cells anticipated to be required for widespread patient access.