Patents by Inventor Yonehiro Kanemura
Yonehiro Kanemura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20200056158Abstract: Disclosed are a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.Type: ApplicationFiled: July 23, 2019Publication date: February 20, 2020Applicants: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa KATO, Yonehiro KANEMURA, Tomoko SHOFUDA, Hayato FUKUSUMI
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Patent number: 10415018Abstract: This invention relates to a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.Type: GrantFiled: August 2, 2018Date of Patent: September 17, 2019Assignees: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa Kato, Yonehiro Kanemura, Tomoko Shofuda, Hayato Fukusumi
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Patent number: 10377984Abstract: It is an object of the present invention to provide a cell culture medium capable of enhancing cell growth efficiency without using feeder cells, in particular which does not comprise serum. The present invention provides a cell culture medium which comprises growth arrest-specific 6 (GAS6) and does not comprise serum.Type: GrantFiled: November 21, 2014Date of Patent: August 13, 2019Assignees: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa Kato, Yonehiro Kanemura, Tomoko Shofuda, Hayato Fukusumi
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Publication number: 20180355324Abstract: This invention relates to a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.Type: ApplicationFiled: August 2, 2018Publication date: December 13, 2018Applicants: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa KATO, Yonehiro KANEMURA, Tomoko SHOFUDA, Hayato FUKUSUMI
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Publication number: 20180320130Abstract: It is an object of the present invention to provide a cell culture medium capable of enhancing cell growth efficiency without using feeder cells, in particular which does not comprise serum. The present invention provides a cell culture medium which comprises growth arrest-specific 6 (GAS6) and does not comprise serum.Type: ApplicationFiled: July 10, 2018Publication date: November 8, 2018Applicants: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa KATO, Yonehiro KANEMURA, Tomoko SHOFUDA, Hayato FUKUSUMI
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Patent number: 10066211Abstract: This invention relates to a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.Type: GrantFiled: June 3, 2014Date of Patent: September 4, 2018Assignees: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa Kato, Yonehiro Kanemura, Tomoko Shofuda, Hayato Fukusumi
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Publication number: 20170226471Abstract: It is an object of the present invention to provide a cell culture medium capable of enhancing cell growth efficiency without using feeder cells, in particular wherein the cell culture medium does not comprise serum. According to the present invention, a cell culture medium comprising fibrin 5 and Zeta polypeptide is provided.Type: ApplicationFiled: August 19, 2015Publication date: August 10, 2017Applicants: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa KATO, Yonehiro KANEMURA, Tomoko SHOFUDA, Hayato FUKUSUMI
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Publication number: 20160376549Abstract: It is an object of the present invention to provide a cell culture medium capable of enhancing cell growth efficiency without using feeder cells, in particular which does not comprise serum. The present invention provides a cell culture medium which comprises growth arrest-specific 6 (GAS6) and does not comprise serum.Type: ApplicationFiled: November 21, 2014Publication date: December 29, 2016Applicants: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa KATO, Yonehiro KANEMURA, Tomoko SHOFUDA, Hayato FUKUSUMI
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Publication number: 20160177272Abstract: This invention relates to a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.Type: ApplicationFiled: June 3, 2014Publication date: June 23, 2016Applicants: KANEKA CORPORATION, NATIONAL HOSPITAL ORGANIZATIONInventors: Tomohisa KATO, Yonehiro KANEMURA, Tomoko SHOFUDA, Hayato FUKUSUMI
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Patent number: 7753054Abstract: A method for creating a monkey model of spinal cord injury, which includes exposing the dura mater of the cervical cord of a monkey and applying a load on the dura mater; the thus-created monkey model of spinal cord injury; and a method for evaluating a therapeutic drug for spinal cord injury by use of this model. According to the present invention, it is possible to create a monkey which is close to the human and thus useful as a model of human spinal cord injury. This model enables proper evaluation of therapeutic effects of various drugs on spinal cord injury. Through use of this model, it has been confirmed for the first time that transplantation therapy of human neural stem cells is efficacious against spinal cord injury.Type: GrantFiled: November 26, 2002Date of Patent: July 13, 2010Assignees: Japan Science and Technology Agency, Keio UniversityInventors: Hideyuki Okano, Yoshiaki Toyama, Masaya Nakamura, Tatsuji Nomura, Yoshikuni Tanioka, Kiyoshi Ando, Yonehiro Kanemura
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Publication number: 20100167398Abstract: Provided are a method of culturing pluripotent stem cells in the presence of a decidua-derived cell or an extracellular matrix derived from the cell, that enables safe and efficient maintenance culture and derivation of pluripotent stem cells; a culture agent for pluripotent stem cells, that comprises a decidua-derived cell or an extracellular matrix derived from the cell; and other means for developing or performing the method.Type: ApplicationFiled: December 23, 2009Publication date: July 1, 2010Applicant: RIKENInventors: Yoshiki SASAI, Tomoko NAGASE, Morio UENO, Yonehiro KANEMURA
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Publication number: 20070031894Abstract: It is an object of the present invention to provide a method for detecting nerve stem cells/progenitor cells among a tissue or a mass of cells as constituted of a plurality of cell species, and a method for screening for a drug functioning as a nerve differentiation factor or the like by using the above-mentioned detection method. It was found in this invention that the expression of the NC1 gene was high in undifferentiated nerve stem cells and said expression decreased with an advance of differentiation. Furthermore in this invention, by using the NC1 gene, the product thereof (NC1 protein) and the base sequences or the amino acid sequences thereof, antibodies or the like, it became possible to detect nerve stem cells/progenitor cells by way of various methods, so that a novel method for screening for a drug functioning as a nerve differentiation factor or the like was provided.Type: ApplicationFiled: September 10, 2004Publication date: February 8, 2007Inventors: Tomoko Syofuda, Yonehiro Kanemura, Jun Miyake, Hideo Niwa, Kenji Yamashita
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Publication number: 20050186545Abstract: A method for creating a monkey model of spinal cord injury, which includes exposing the dura mater of the cervical cord of a monkey and applying a load on the dura mater; the thus-created monkey model of spinal cord injury; and a method for evaluating a therapeutic drug for spinal cord injury by use of this model. According to the present invention, it is possible to create a monkey which is close to the human and thus useful as a model of human spinal cord injury. This model enables proper evaluation of therapeutic effects of various drugs on spinal cord injury. Through use of this model, it has been confirmed for the first time that transplantation therapy of human neural stem cells is efficacious against spinal cord injury.Type: ApplicationFiled: November 26, 2002Publication date: August 25, 2005Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Hideyuki Okano, Yoshiaki Toyama, Masaya Nakamura, Tatsuji Nomura, Yoshikuni Tanioka, Kiyoshi Ando, Yonehiro Kanemura
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Publication number: 20030186335Abstract: The present invention provides monoclonal antibodies useful in selection/isolation/preparation of a cell derived from a nerve tissue or other tissue, and in the examination of site-specific expression of proteins in brain nerve tissue. The present invention also provides hybridomas which produce the monoclonal antibodies, a method for isolation of a cell derived from a nerve tissue or other tissue using the monoclonal antibodies, a cell isolated through the method, and an immunological diagnostic method using the monoclonal antibodies.Type: ApplicationFiled: March 27, 2003Publication date: October 2, 2003Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Masayuki Hara, Yonehiro Kanemura, Jun Miyake, Hideyuki Okano, Mami Yamasaki, Yasuhiro Nakamura, Munehiko Yamamoto, Eriko Oda, Eri Kodama