Patents by Inventor Yong Ho Ahn
Yong Ho Ahn has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11912924Abstract: The present invention provides a composition for antifreezing including a gold (Au) nanostructure in which at least a portion thereof is concave, thereby it is possible to increase a survival rate of cells due to having excellent effect of inhibiting ice recrystallization when cryopreservation of the cells, and maintain a texture of food even when using in the freezing of food.Type: GrantFiled: December 3, 2019Date of Patent: February 27, 2024Assignee: Korea University Research and Business FoundationInventors: Dong June Ahn, Sang Yup Lee, Yong Ho Cho
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Patent number: 11046975Abstract: The present invention relates to a bicistronic expression vector for antibody expression, an animal cell transfected with the expression vector, and a method for producing an antibody including culturing the animal cell, in which the expression vector includes a first expression cassette including ‘promoter-UTR-intron-antibody light chain gene-polyA’ and a second expression cassette including ‘promoter-UTR-intron-antibody heavy chain gene-internal ribosome entry site (IRES)-amplification gene-polyA’. An expression vector capable of expressing a desired antibody with high efficiency can be constructed using the bicistronic expression vector including an intron for antibody expression according to the present invention, and the expression vector can produce the antibody by culturing the transfected animal cell with stability and high efficiency.Type: GrantFiled: October 7, 2014Date of Patent: June 29, 2021Assignee: PRESTIGE BIOPHARMA PTE. LTD.Inventors: Soo Kwang Kim, Young Min Kim, Yong Gyu Son, Yong Ho Ahn, Dong Heon Lee, Yang Soon Lee, Hee Jung Jun, Yu Bin Choi, Eun A Kim
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Patent number: 10808272Abstract: Provided are a method of preparing an antibody with a regulated, sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content, a method of preparing a high-quality population of antibodies by regulating the sugar content of the antibody to a desired content, and a population of antibodies prepared by the method. Further, provided is a method of regulating the antibody sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content. Furthermore, provided is a medium composition, for regulating the antibody sugar chain content, the medium composition including glycerol as an additive for regulating the antibody sugar chain content.Type: GrantFiled: April 2, 2015Date of Patent: October 20, 2020Assignee: Prestige Biopharma PTE. LTD.Inventors: Jae Young Chang, Eun Ho Hwang, Yong Jin Kim, Won Kyum Kim, Sang Kyung Park, Jun Yong Park, Kyo Eun Ahn, Yong Ho Ahn, Ji Yong Yoon, Jung Woo Lee
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Patent number: 10053489Abstract: The present invention relates to a method of purifying an antibody with high purity and high quality by removing impurities by sequential use of a cation-exchange column, a culture supernatant multilayer filter and an anion-exchange column without using a protein-A column that is an affinity chromatography column which is generally used for antibody purification.Type: GrantFiled: June 3, 2014Date of Patent: August 21, 2018Assignee: Prestige Biopharma PTE, LTDInventors: Soo Kwang Kim, Yong Ho Ahn, Young Min Kim, Dae Hae Song
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Publication number: 20170211057Abstract: The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particulary, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformat comprising the vector, and a method for preparing a target protein using the transformat.Type: ApplicationFiled: January 5, 2017Publication date: July 27, 2017Inventors: Hyun Sook Jang, Dong Heon Lee, Sun Kyu Kim, Yong Ho Ahn, Sang Kyung Park
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Patent number: 9700595Abstract: The present invention relates to a liquid formulation comprising a TNFR-Fc fusion protein and a stabilizer, in which the fusion protein comprises TNFR (tumor necrosis factor receptor) or a fragment thereof and an immunoglobulin Fc region, and the stabilizer comprises one or more amino acids selected from the group consisting of proline and histidine, a buffer solution, and an isotonic agent containing sodium chloride (NaCl) and sucrose, and a preparation method of the liquid formulation. The liquid formulation according to the present invention provides excellent storage stability because long-term storage of TNFR-Fc fusion protein (etanercept) is possible and particular storage conditions are not needed.Type: GrantFiled: November 28, 2014Date of Patent: July 11, 2017Assignee: ARES TRADING SAInventors: Jung Tae Lee, In Hyuk Kim, Jae Keun Yu, Jung Yim Yim, Myeong Hyeon Jeong, Yong Ho Ahn
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Publication number: 20170107551Abstract: Provided are a method of preparing an antibody with a regulated, sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content, a method of preparing a high-quality population of antibodies by regulating the sugar content of the antibody to a desired content, and a population of antibodies prepared by the method. Further, provided is a method of regulating the antibody sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content. Furthermore, provided is a medium composition, for regulating the antibody sugar chain content, the medium composition including glycerol as an additive for regulating the antibody sugar chain content.Type: ApplicationFiled: April 2, 2015Publication date: April 20, 2017Applicant: Prestige Biopharma PTE. LTD.Inventors: Jae Young Chang, Eun Ho Hwang, Yong Jin Kim, Won Kyum Kim, Sang Kyung Park, Jun Yong Park, Kyo Eun Ahn, Yong Ho Ahn, Ji Yong Yoon, Jung Woo Lee
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Patent number: 9567577Abstract: The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particularly, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformant comprising the vector, and a method for preparing a target protein using the transformant.Type: GrantFiled: March 5, 2013Date of Patent: February 14, 2017Assignee: ARES TRADING S.A.Inventors: Hyun Sook Jang, Dong Heon Lee, Sun Kyu Kim, Yong Ho Ahn, Sang Kyung Park
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Publication number: 20170028020Abstract: The present invention relates to a liquid formulation comprising a TNFR-Fc fusion protein and a stabilizer, in which the fusion protein comprises TNFR (tumor necrosis factor receptor) or a fragment thereof and an immunoglobulin Fc region, and the stabilizer comprises one or more amino acids selected from the group consisting of proline and histidine, a buffer solution, and an isotonic agent containing sodium chloride (NaCl) and sucrose, and a preparation method of the liquid formulation. The liquid formulation according to the present invention provides excellent storage stability because long-term storage of TNFR-Fc fusion protein (etanercept) is possible and particular storage conditions are not needed.Type: ApplicationFiled: November 28, 2014Publication date: February 2, 2017Inventors: Jung Tae Lee, In Hyuk Kim, Jae Keun Yu, Jung Yim Yim, Myeong Hyeon Jeong, Yong Ho Ahn
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Publication number: 20160281106Abstract: The present invention relates to a bicistronic expression vector for antibody expression, an animal cell transfected with the expression vector, and a method for producing an antibody including culturing the animal cell, in which the expression vector includes a first expression cassette including ‘promoter-UTR-intron-antibody light chain gene-polyA’ and a second expression cassette including ‘promoter-UTR-intron-antibody heavy chain gene-internal ribosome entry site (IRES)-amplification gene-polyA’. An expression vector capable of expressing a desired antibody with high efficiency can be constructed using the bicistronic expression vector including an intron for antibody expression according to the present invention, and the expression vector can produce the antibody by culturing the transfected animal cell with stability and high efficiency.Type: ApplicationFiled: October 7, 2014Publication date: September 29, 2016Inventors: Soo Kwang Kim, Young Min Kim, Yong Gyu Son, Yong Ho Ahn, Dong Heon Lee, Yang Soon Lee, Hee Jung Jun, Yu Bin Choi, Eun A Kim
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Publication number: 20160122384Abstract: The present invention relates to a method of purifying an antibody with high purity and high quality by removing impurities by sequential use of a cation-exchange column, a culture supernatant multilayer filter and an anion-exchange column without using a protein-A column that is an affinity chromatography column which is generally used for antibody purification.Type: ApplicationFiled: June 3, 2014Publication date: May 5, 2016Inventors: Soo Kwang KIM, Yong Ho AHN, Young Min KIM, Dae Hae SONG
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Patent number: 9279014Abstract: The present invention relates to a method for separating and preparing a TNFR-Fc fusion protein using hydrophobic interaction chromatography (HIC). More particularly, the present invention relates to a method for separating and preparing a highly pure active protein from clipped proteins due to displacement effect by adjusting the conductivity of a protein sample using a high concentration of salt solution and by adjusting a loading amount thereof, and a TNFR-Fc fusion protein prepared by the method.Type: GrantFiled: August 17, 2012Date of Patent: March 8, 2016Assignee: ARES TRADING S.A.Inventors: Hye Soon Won, Byung Je Sung, Yong Ho Ahn, Sang Kyung Park
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Publication number: 20150044753Abstract: The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particularly, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformant comprising the vector, and a method for preparing a target protein using the transformant.Type: ApplicationFiled: March 5, 2013Publication date: February 12, 2015Inventors: Hyun Sook Jang, Dong Heon Lee, Sun Kyu Kim, Yong Ho Ahn, Sang Kyung Park
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Publication number: 20140316114Abstract: The present invention relates to a method for separating and preparing a TNFR-Fc fusion protein using hydrophobic interaction chromatography (HIC). More particularly, the present invention relates to a method for separating and preparing a highly pure active protein from clipped proteins due to displacement effect by adjusting the conductivity of a protein sample using a high concentration of salt solution and by adjusting a loading amount thereof, and a TNFR-Fc fusion protein prepared by the method.Type: ApplicationFiled: August 17, 2012Publication date: October 23, 2014Applicant: HANWHA CHEMICAL CORPORATIONInventors: Hye Soon Won, Byung Je Sung, Yong Ho Ahn, Sang Kyung Park
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Publication number: 20110159193Abstract: Disclosed herein is a method of forming a flexible moisture and oxygen barrier thin film substrate for a flexible display and food packaging, the thin film being able to increase the life-spans of organic devices. The method includes the steps of: a) uniformly dispersing plate-shape nanometer-size or micrometer-size particles in polymer solution; b) casting the polymer solution dispersed with the nanometer-size or micrometer-size particles using the solution casting method and then removing the solvent from the cast polymer solution to form a plastic film; c) stretching the formed plastic film between the glass transition temperature and melting point to exfoliate the plate-shape nanometer-size or micrometer-size particles and to orient the exfoliated nanometer-size or micrometer-size particles; d) coating the stretched plastic film with an organic film to smooth the surface of the flexible substrate; and e) heat-treating the flexible substrate to cure the organic film.Type: ApplicationFiled: December 27, 2010Publication date: June 30, 2011Inventors: Choon Sup Yoon, Jin Hae Chang, Yong Ho Ahn
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Patent number: 7655155Abstract: A liquid crystal thermoset (LCT) monomer or oligomer having both ends capped with maleimide having at least one methyl group, represented by Formula 1: wherein R1 and R2 are each independently CH3 or H, and at least one of R1 and R2 is a methyl group, and Ar1 is a divalent organic group containing one or more structural units selected from the group consisting of ester, amide, ester amide, ester imide and ether imide units, and Ar1 has a molecular weight not greater than 5,000.Type: GrantFiled: July 15, 2008Date of Patent: February 2, 2010Assignees: Samsung Electronics Co., Ltd., Samsung Electro-Mechanics Co., Ltd., Samsung Fine Chemicals Co., Ltd.Inventors: Myung Sup Jung, Chung Kun Cho, Yong Ho Ahn, Seo Won Jang, Jin Hae Chang, Mahn Jong Kim, Chung Won Park
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Publication number: 20090224203Abstract: A liquid crystal thermoset (LCT) monomer or oligomer having both ends capped with maleimide having at least one methyl group, represented by Formula 1: wherein R1 and R2 are each independently CH3 or H, and at least one of R1 and R2 is a methyl group, and Ar1 is a divalent organic group containing one or more structural units selected from the group consisting of ester, amide, ester amide, ester imide and ether imide units, and Ar1 has a molecular weight not greater than 5,000.Type: ApplicationFiled: July 15, 2008Publication date: September 10, 2009Applicants: SAMSUNG ELECTRONICS CO., LTD., SAMSUNG ELECTRO-MECHANICS CO., LTD., SAMSUNG FINE CHEMICALS CO., LTD.Inventors: Myung Sup Jung, Chung Kun Cho, Mahn Jong Kim, Chung Won Park, Yong HO Ahn, Seo Won Jang, Jin Hae Chang