Abstract: An assay device and method of use thereof, include a multiwell chip and a microarray slide printed with capture molecules. The chip contains shallow wells for holding liquid samples and, optionally, shoulders for receiving the slide. The slide, when placed on the chip, can contact the top of the liquid sample and draw the liquid sample upwards onto the capture molecules. The microarray slide and multiwell chip are capable of forming a closed incubation chamber preventing outside contamination and liquid evaporation. Patterning of the slide can be carried out by direct write lithography or nanolithography, including DPN printing. Small liquid volumes can be used.